PRP8A and PRP8B spliceosome subunits act coordinately to control pollen tube attraction in <i>Arabidopsis thaliana</i>

Kulichova, Katarina; Kumar, Vinod; Steinbachová, Lenka; Klodová, Božena; Timofejeva, Ljudmilla; Juříček, Miloslav; Honys, David; Hafidh, Said

doi:10.1242/dev.186742

Cited by 13 publications

(13 citation statements)

References 92 publications

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“…Monitoring of pollen tube guidance in the presence of galvestine-1 was conducted through semi in vivo assays on plates containing 1% DMSO for mock treatment or 5 or 10 µM of galvestine-1 with a final concentration of 1% DMSO. For all pollen tube guidance assays, in vivo aniline blue staining and semi in vivo as well as in vivo blue dot assays were performed according to (Kulichová et al, 2020).…”

Section: Pollen Phenotypingmentioning

confidence: 99%

LARP6C orchestrates posttranscriptional reprogramming of gene expression during hydration to promote pollen tube guidance

et al. 2021

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Increasing evidence suggests that post-transcriptional regulation is a key player in the transition between mature pollen and the progamic phase (from pollination to fertilization). Nonetheless, the actors in this mRNA-based gene expression reprogramming are poorly understood. We demonstrate that the evolutionarily conserved RNA-binding protein (RBP) LARP6C is necessary for the transition from dry pollen to pollen tubes and the guided growth of pollen tubes towards the ovule in Arabidopsis thaliana. In dry pollen, LARP6C binds to transcripts encoding proteins that function in lipid synthesis and homeostasis, vesicular trafficking, and polarized cell growth. LARP6C also forms cytoplasmic granules that contain the poly(A) binding protein and possibly represent storage sites for translationally silent mRNAs. In pollen tubes, loss of LARP6C negatively affects the quantities and distribution of storage lipids, as well as vesicular trafficking. In Nicotiana benthamiana leaf cells and in planta, analysis of reporter mRNAs designed from the LARP6C target MGD2 provided evidence that LARP6C can shift from a repressor to an activator of translation when the pollen grain enters the progamic phase. We propose that LARP6C orchestrates the timely post-transcriptional regulation of a subset of mRNAs in pollen during the transition from the quiescent to active state and along the progamic phase to promote male fertilization in plants.

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Section: Pollen Phenotypingmentioning

confidence: 99%

LARP6C orchestrates posttranscriptional reprogramming of gene expression during hydration to promote pollen tube guidance

et al. 2021

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“…Recent studies indicated that misregulation of the spliceosome complex leads to defective development of the male and female gametophytes resulting in embryo lethality in Arabidopsis (Kulichová et al ., 2020; Slane et al ., 2020). Both high temperatures and altered ABA levels in seeds may regulate the levels of the spliceosome components (Cruz et al , 2014; Jiang et al , 2017).…”

Section: Resultsmentioning

confidence: 99%

Effects of long-term high-temperature stress on reproductive growth and seed development in development in Brassica napus

Mácová

Prabhullachandran

Štefková

et al. 2021

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Brassica napus is the second most important oilseed crop worldwide. Increasing average temperatures and extreme weather have a severe impact on rapeseed yield. We determined the response of three cultivars to different temperature regimes (21/18 °C, 28/18 °C and 34/18 °C), focusing on the plant appearance, seed yield, seed quality, seed viability, and embryo development. Our microscopic observations identified that embryo development is affected by high temperatures. We noticed an acceleration of its development, in addition to pattern defects. Reduced fertilization rate, increased abortion rate, and preharvest sprouting would be responsible for the low seed yield at the high-temperature regime. Hormone profiling indicates that reduced auxin levels in young seeds may cause the observed embryo pattern defects. Moreover, reduced seed dormancy may result from low ABA and IAA levels in mature seeds. Glucosinolates and oil composition measurements suggest reduced seed quality. These identified cues help understand seed thermomorphogenesis and pave the way to the development of thermoresilient rapeseed plants.

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“…PRP8A is more highly expressed than PRP8B , and prp8a null mutations are lethal ( Schwartz et al, 1994 ; Marquardt et al, 2014 ). In contrast, insertional alleles of the less-expressed PRP8B are viable but display female gametophytic defects in combination with prp8a mutants ( Kulichová et al, 2020 ). Several viable prp8a missense alleles displaying reduced splicing efficiency have been characterized, including prp8a-6 , prp8a-7 , prp8a-10 , and prp8a-11 ( Marquardt et al, 2014 ; Sasaki et al, 2015 ; Kanno et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

An Arabidopsispre-RNA processing8a (prp8a)missense allele restores splicing of a subset of mis-spliced mRNAs

et al. 2022

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Eukaryotic precursor mRNAs often harbor noncoding introns that must be removed prior to translation. Accurate splicing of precursor messenger RNA depends on placement and assembly of small nuclear ribonucleoprotein (snRNP) sub-complexes of the spliceosome. Yeast (Saccharomyces cerevisiae) studies established a role in splice-site selection for PRE-RNA PROCESSING8 (PRP8), a conserved spliceosome scaffolding protein of the U5 snRNP. However, analogous splice-site selection studies in multicellular eukaryotes are lacking. Such studies are crucial for a comprehensive understanding of alternative splicing, which is extensive in plants and animals but limited in yeast. In this work, we describe an Arabidopsis (Arabidopsis thaliana) prp8a mutant that modulates splice-site selection. We isolated prp8a-14 from a screen for suppressors of pex14-6, which carries a splice-site mutation in the PEROXIN14 (PEX14) peroxisome biogenesis gene. To elucidate Arabidopsis PRP8A function in spliceosome fidelity, we combined prp8a-14 with various pex14 splice-site mutations and monitored the double mutants for physiological and molecular consequences of dysfunctional and functional peroxisomes that correspond to impaired and recovered splicing, respectively. prp8a-14 restored splicing and PEX14 function to alleles with mutations in the exonic guanine of the 5′-splice site but did not restore splicing or function to alleles with mutations in the intronic guanine of 5′- or 3′-splice sites. We used RNA-seq to reveal the systemic impact of prp8a-14 and found hundreds of differentially spliced transcripts and thousands of transcripts with significantly altered levels. Among differentially spliced transcripts, prp8a-14 significantly altered 5′- and 3′-splice-site utilization to favor sites resulting in shorter introns. This study provides a genetic platform for probing splicing in plants and hints at a role for plant PRP8 in splice-site selection.

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PRP8A and PRP8B spliceosome subunits act coordinately to control pollen tube attraction in Arabidopsis thaliana

Cited by 13 publications

References 92 publications

LARP6C orchestrates posttranscriptional reprogramming of gene expression during hydration to promote pollen tube guidance

LARP6C orchestrates posttranscriptional reprogramming of gene expression during hydration to promote pollen tube guidance

Effects of long-term high-temperature stress on reproductive growth and seed development in development in Brassica napus

An Arabidopsispre-RNA processing8a (prp8a)missense allele restores splicing of a subset of mis-spliced mRNAs

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