Prp19 Arrests Cell Cycle via Cdc5L in Hepatocellular Carcinoma Cells

Huang, Ruoyu; Xue, Ruyi; Qu, Di; Yin, Jie; Shen, Xizhong

doi:10.3390/ijms18040778

Cited by 19 publications

(18 citation statements)

References 27 publications

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“…CDC5L was involved in cell cycle regulation, and CDC5L inhibition in cells arrested the cell cycle at G2/M phase [25]. In bladder cancer, CDC5L expression was obviously increased and CDC5L expression was significantly related to pathology grade and Ki67 expression in bladder cancer.…”

Section: Discussionmentioning

confidence: 97%

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

Liu

Guo

Sha

et al. 2020

Aging

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This study aimed to investigate whether annexin A7 (ANXA7) could promote the cell cycle, proliferation and cell adhesion-mediated drug resistance (CAM-DR) of multiple myeloma (MM) cells by up-regulating cell division cycle 5-like (CDC5L). As a result, ANXA7 expression was increased in the serum of MM patients and the expression of ANXA7 and CDC5L was also increased in MM cell lines. ANXA7 overexpression promoted the proliferation and cycle of U266 and RPMI8226 cells. The expression of proliferation cell nuclear antigen (PCNA), KI67, cyclin dependent kinase 1 (CDK1) and cyclinB1 in transfected cells was consistent with the changes of proliferation and cell cycle. In co-culture system of BMSC cells and MM cells, expression of CD44, ICAM1 and VCAM1 in MM cells was increased, which was further increased by ANXA7 overexpression. Bortezomib could increase the apoptosis of U266 and RPMI8226 cells. In co-culture system of BMSC cells and MM cells, the promotion effects of bortezomib on apoptosis of MM cells was decreased, which was further suppressed by ANXA7 overexpression. The above effects exerted by ANXA7 overexpression could be reversed by ANXA7 interference. Moreover, ANXA7 was proved to be combined with CDC5L. CDC5L interference could inhibit the promotion effects of ANXA7 overexpression on proliferation and cell cycle and inhibition effects of ANXA7 overexpression on apoptosis of MM cells treated with bortezomib in co-culture system. In conclusion, ANXA7 could promote the cell cycle, proliferation and CAM-DR of MM cells by upregulating CDC5L.

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Section: Discussionmentioning

confidence: 97%

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

Liu

Guo

Sha

et al. 2020

Aging

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“…[87] (Figure 1). Depletion of CDC5L causes dramatic mitotic arrest, chromosome 220 misalignments and sustained activation of spindle assembly checkpoint [85, [88][89][90]. The mechanism can be inferred from data showing that CDC5L modulates the pre-mRNA splicing 222 of a set of genes involved in mitosis [89].…”

Section: Phase 178mentioning

confidence: 99%

Splicing to Keep Cycling: The Importance of Pre-mRNA Splicing during the Cell Cycle

et al. 2021

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Pre-mRNA splicing is a fundamental process in mammalian gene expression and alternative splicing plays an extensive role in generating protein diversity. Since the majority of genes undergo pre-mRNA splicing, most cellular processes depend on proper spliceosome function.Here we focus on the cell cycle and describe its dependence on pre-mRNA splicing and accurate alternative splicing. We outline the key cell cycle factors and their known alternative splicing isoforms. We discuss different levels of pre-mRNA splicing regulation such as posttranslational modifications and changes in expression of splicing factors. We describe the effect of chromatin dynamics on pre-mRNA splicing during the cell cycle. In addition, we focus on the spliceosome component SF3B1, which is mutated in many types of cancer, and describe the link of SF3B1 and its inhibitors to cell cycle.

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“…Cell division cycle 5-like (Cdc5L) is a core component protein in the pre-messenger RNA (mRNA) processing factor 19 associated complex, which is a putative E3 ubiquitin ligase complex and is functional in pre-mRNA processing and DNA damage response ( Zhang et al, 2009 ; Huang et al, 2017 ). Previous studies have shown that Cdc5L contributed to the progression of various cancers, such as colorectal cancer ( Li et al, 2017 ), prostate cancer ( Li et al, 2018 ), gliomas ( Chen et al, 2016 ), bladder cancer ( Zhang et al, 2020 ), and hepatocellular carcinoma ( Qiu et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Cell Division Cycle 5-Like Regulates Metaphase-to-Anaphase Transition in Meiotic Oocyte

Zhang

Ouyang

et al. 2021

Front. Cell Dev. Biol.

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The quality of oocytes is a vital factor for embryo development. Meiotic progression through metaphase I usually takes a relatively long time to ensure correct chromosome separation, a process that is critical for determining oocyte quality. Here, we report that cell division cycle 5-like (Cdc5L) plays a critical role in regulating metaphase-to-anaphase I transition during mouse oocyte meiotic maturation. Knockdown of Cdc5L by small interfering RNA injection did not affect spindle assembly but caused metaphase I arrest and subsequent reduced first polar body extrusion due to insufficient anaphase-promoting complex/cyclosome activity. We further showed that Cdc5L could also directly interact with securin, and Cdc5L knockdown led to a continuous high expression level of securin, causing severely compromised meiotic progression. The metaphase-to-anaphase I arrest caused by Cdc5L knockdown could be rescued by knockdown of endogenous securin. In summary, we reveal a novel role for Cdc5L in regulating mouse oocyte meiotic progression by interacting with securin.

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Prp19 Arrests Cell Cycle via Cdc5L in Hepatocellular Carcinoma Cells

Cited by 19 publications

References 27 publications

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

Splicing to Keep Cycling: The Importance of Pre-mRNA Splicing during the Cell Cycle

Cell Division Cycle 5-Like Regulates Metaphase-to-Anaphase Transition in Meiotic Oocyte

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