PRP of T2DM Patient Immobilized on PCL Nanofibers Stimulate Endothelial Cells Proliferation

Abstract

Diabetic foot ulcers (DFU) are a common complication of Type 2 Diabetes Mellitus (T2DM). Development of bioactive wound healing covers is an important task in medicine. The use of autologous platelet-rich plasma (PRP) consisting of growth factors, cytokines and components of extracellular matrix is a perspective approach for DFU treatment, but we previously found that some T2DM PRP samples have a toxic effect on mesenchymal stem cells (MSCs) in vitro. Here, we covalently immobilized T2DM PRP proteins on polycaprolactone (PCL) nanofibers, and the growth of endothelial cells on the PCL-COOH-PRP was investigated. Additionally, the level of NO reflecting the cytotoxic effects of PRP, angiogenin, and VEGF levels were measured in T2DM PRP samples. The results showed that the application of PCL-COOH-PRP nanofibers allows to remove the cytotoxicity of T2DM PRP and to improve endothelial cell adhesion and proliferative activity. We showed that the origin of T2DM PRP (the level of PRP toxicity or presence/absence of DFU) does not influence the efficiency of cell growth on PCL-COOH-PRP, and on the level of angiogenin, vascular epidermal growth factor (VEGF) in PRP itself.

3D-printed PCL scaffolds with anatomy-inspired bionic stratified structures for the treatment of growth plate injuries

“…Traditional modification methods for PCL materials include RGD surface modification [ 43 ], platelet-rich plasma coating [ 44 ], Polydopamine coating [ 45 ]. However, these methods often involve expensive reagents and complex procedures, making it challenging to translate them to clinical applications [ 46 ].…”

Recent Developments in the use of Plasma for Therapeutic Applications

“…A plasma-deposited layer of acrylic acid enhanced the adhesion of platelet-rich plasma onto polycaprolactone fibers. The inclusion of the acid groups enhanced cell adhesion and proliferation [49]. In addition to the materials described above, plasma-deposited allylamine enhanced the adhesion of functional proteins onto ceramic dental implants [50], thereby demonstrating the wide range of materials that can be treated with such an approach.…”

“…A plasma-deposited layer of acrylic acid enhanced the adhesion of platelet-rich plasma onto polycaprolactone fibers. The inclusion of the acid groups enhanced cell adhesion and proliferation [49].…”

“…Plasma-deposited polymer coatings have been widely studied over many years and are now finding widespread use as binding layers that can be used to attach highly functional biologics onto various scaffolds, dressings and medical implants [47][48][49][50][51]. Even simple plasma coatings can have dramatic effects on medical outcomes.…”