Proximity Ligation Measurement of the Complex between Prostate Specific Antigen and α1-Protease Inhibitor

Zhu, Lei; Koistinen, Hannu; Landegren, Ulf; Stenman, Ulf‐Håkan

doi:10.1373/clinchem.2009.127779

Cited by 20 publications

(10 citation statements)

References 25 publications

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“…Later, an improved assay for PSA-API was developed based on the proximity-ligation principle. While this assay was sensitive enough and eliminated non-specific background enabling accurate measurement of PSA-API in serum specimens with moderately increased PSA concentrations, the complicated assay design prohibited its clinical use 170 .…”

Section: Various Forms Of Prostate-specific Antigen In Prostate Cancermentioning

confidence: 99%

Biomarker development, from bench to bedside

Stenman

2015

Critical Reviews in Clinical Laboratory Sciences

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Application of laser & photonics in dentistry and medicine has a long history, however, it is still emerging as a new field enabling the application of advanced technology at precise size and levels and the exploitation of novel properties. Many areas of dental/medical device are expected to benefit from photonics including sensors for use in the laboratory , the clinic, and within the human body. For example, the potential uses of laser include the early detection and treatment of disease. These new innovative biomedical pho-tonics include "advanced bioimaging" to push photon-based imaging tools to new limits of resolution and utility for "molecular and cellular imaging" to develop and apply advanced optical diagnostic probes to important problems in molecular and cellular biology, and "biomedical photon-ics" to translate photonics to clinical dentistry/medicine for disease screening, diagnosis and therapy. Launched in April 2015, the Japan Agency for Medical Research and Development （AMED） pursues its assigned mission to fast-track medical research and development in support of humanity. AMED is currently tackling the challenge of reforming Japan＇s funding allocation and evaluation systems. Other significant tasks in fast-tracking the social implementation of research outcomes include rapid diagnosis of undiagnosed diseases, and novel therapeutic interventions for patients. AMED engages in research and development in the field of medicine, establishing and maintaining an environment for this R&D, and providing funding, in order to promote integrated medical R&D from basic research to practical applications, to smoothly achieve application of outcomes, and to achieve comprehensive and effective establishment / maintenance of an environment for medical R&D. Providing a one-stop service for research expenses, AMED consolidates budgets for research expenses, which had previously been allocated from different sources-the Ministry of Education, Culture, Sports, Science and Technology , the Ministry of Health, Labour and Welfare, and the Ministry of Economy, Trade and Industry. AMED aims to achieve the world＇s highest level of medical care / service and to form a society in which people live long, healthy lives by promoting integrated research and development, from basic research to practical application, and by establishing and maintaining an environment therefor, and linking this to various forms of growth in medical R&D. In this special lecture I would introduce a birds-eye view not only AMED supporting R&D program but also FMDIPA activity to develop dental/medical laser device industry, currently being undertaken to overcome the problems facing dental/medical diseases. He has made extensive contributions to the development of the bioengineering profession worldwide through his extensive professional activities and service with the government. He has also extensive experience in working with industry partners. In recognition of his contribution and leadership, he was elected for the initiative member to start up t...

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Section: Various Forms Of Prostate-specific Antigen In Prostate Cancermentioning

confidence: 99%

Biomarker development, from bench to bedside

Stenman

2015

Critical Reviews in Clinical Laboratory Sciences

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“…The PLA technique enables accurate visualisation, subcellular localisation, and quantitative evaluation of protein–protein interactions, as, for example, was shown for complexes of prostate specific antigen with α1-protease inhibitor (Zhu et al , 2009), and for EGF receptor–HER2 complex formation in vitro (Leuchowius et al , 2009). …”

Section: Discussionmentioning

confidence: 99%

In situ quantification of HER2–protein tyrosine kinase 6 (PTK6) protein–protein complexes in paraffin sections from breast cancer tissues

et al. 2010

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Background:Protein tyrosine kinase 6 (PTK6; breast tumour kinase) is overexpressed in up to 86% of the invasive breast cancers, and its association with the oncoprotein human epidermal growth factor receptor 2 (HER2) was shown in vitro by co-precipitation. Furthermore, expression of PTK6 in tumours is linked with the expression of HER2.Method and results:In this study, we used the proximity ligation assay (PLA) technique on formalin-fixed paraffin sections from eighty invasive breast carcinoma tissue specimens to locate PTK6–HER2 protein–protein complexes. Proximity ligation assay signals from protein complexes were assessed quantitatively, and expression levels showed a statistically significant association with tumour size (P=0.015) and course of the cancer disease (P=0.012).Conclusion:Protein tyrosine kinase 6 forms protein complexes with HER2 in primary breast cancer tissues, which can be visualised by use of the PLA technique. Human epidermal growth factor receptor 2–PTK6 complexes are of prognostic relevance.

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“…2PLA has also been modified with a proximity extension assay (PEA), where ligation is replaced with extension 33. Various formats of PLA have been used to detect proteins,1, 34 protein complexes,35 and single virus particles;36 to screen for inhibitors of protein–protein interactions;37 and to determine the binding specificity of DNA‐binding proteins (such as p53, HNF‐4α) to different dsDNA sequences 38…”

Section: Proximity Ligation Assaymentioning

confidence: 99%

Assembling DNA through Affinity Binding to Achieve Ultrasensitive Protein Detection

Zhang¹,

Li²,

Dever³

et al. 2013

Angew Chem Int Ed

159

123

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Recent advances in DNA assembly and affinity binding have enabled exciting developments of nanosensors and ultrasensitive assays for specific proteins. These sensors and assays share three main attractive features: 1) the detection of proteins can be accomplished by the detection of amplifiable DNA, thereby dramatically enhancing the sensitivity; 2) assembly of DNA is triggered by affinity binding of two or more probes to a single target molecule, thereby resulting in increased specificity; and 3) the assay is conducted in solution with no need for separation, thus making the assay attractive for potential point-of-care applications. We illustrate here the principle of assembling DNA through affinity binding, and we highlight novel applications to the detection of proteins.

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Proximity Ligation Measurement of the Complex between Prostate Specific Antigen and α1-Protease Inhibitor

Cited by 20 publications

References 25 publications

Biomarker development, from bench to bedside

Biomarker development, from bench to bedside

In situ quantification of HER2–protein tyrosine kinase 6 (PTK6) protein–protein complexes in paraffin sections from breast cancer tissues

Assembling DNA through Affinity Binding to Achieve Ultrasensitive Protein Detection

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