Proximate body composition analysis implies percent composition of moisture, lipids and protein. The aim of the study of to evaluate the effect of the various levels of dietary protein on the body composition of Labeo calbasu. Fish were fed with 15%, 20% and 25% crude protein in diet in treatment 1 (T1), treatment 2 (T2) and treatment 3 (T3), respectively. At the end of feeding trial, a total of 120 samples of Labeo calbasu, 40 belonging to each of the three dietary protein treatments were evaluated for proximate body composition. Mean water, ash, fat and protein in wet weigh of L. calbasu were ranged 76.59-77.19%, 3.63-4.68%, 4.08-4.86% and 13.87-15.66%, respectively, in different treatments. Ash and fat percentages were found highest in T1, water parentage was found highest in T2, while mean protein content percentage value was found highest in T3 (25% crude protein). All the body constituents were found to be highly correlated (P<0.001) with the fish size (length and weight) in T1, T2 and T3, except for ash contents in T2 (r = 0.472) and T3 (r = 0.412) showing correlation at P<0.01. Slope (b value) indicated positive correlations in all the relationships between fish size and different constituents. No studies on L. calbasu have yet attempted to evaluate the effect of dietary protein levels on body composition and to relate the size of the fish with the parameters of whole body composition. Thus, this study will provide the first reference from Pakistan.