Proximate composition and biological activity of food plants gathered by chilean Amerindians

Schmeda‐Hirschmann, Guillermo; Razmilic, Iván; Gutiérrez, Margarita; Loyola, J. I.

doi:10.1007/bf02866496

Cited by 48 publications

(36 citation statements)

References 24 publications

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“…Spectrophotometric batch experiments DPPH solutions in pure methanol and ethanol exhibited maximum absorption at 517 nm which is in good accordance with earlier data [5,6,7,8,9,10]. For practical (solubility) reasons the lyophilised plant extracts are usually recovered in aqueous 50% ethanol; a shift of the absorption maximum to 525 nm was observed in this solvent and the stability of 0.5 mM DPPH in the absence of antioxidants was even better (~1% decrease of absorbance over 1 h) than in commonly used pure methanol (~2% over 1 h).…”

Section: Resultssupporting

confidence: 77%

“…[5]) that are suitable for assessing radical-inactivating effect of antioxidants present in relatively complex samples such as herbal extracts. For example, the quenching of coloured diphenylpicrylhydrazyl radical (DPPH) caused by its interaction with antioxidants in organic or aqueous-organic solutions can be easily followed by spectrophotometry [6,7,8]. Batch version of the DPPH quenching method does not facilitate screening of large series of plant samples since it is rather time consuming, the standard reaction time being 16-30 min [7,8,9].…”

Section: Introductionmentioning

confidence: 99%

“…For example, the quenching of coloured diphenylpicrylhydrazyl radical (DPPH) caused by its interaction with antioxidants in organic or aqueous-organic solutions can be easily followed by spectrophotometry [6,7,8]. Batch version of the DPPH quenching method does not facilitate screening of large series of plant samples since it is rather time consuming, the standard reaction time being 16-30 min [7,8,9]. Some recent papers show the feasibility of combining HPLC separation with on-line post-column detection of antioxidants in complex mixtures with DPPH as reagent [5,10]; the analysis of a single plant extract takes about 30 min [5] so its use for processing numerous samples in routine screening tests is not practical.…”

Section: Introductionmentioning

confidence: 99%

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Rapid automated assay of anti-oxidation/radical-scavenging activity of natural substances by sequential injection technique (SIA) using spectrophotometric detection

et al. 2004

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A PC-controlled sequential injection analysis (SIA) system equipped with a spectrophotometric diode-array detector is used for rapid monitoring and evaluation of antioxidation/radical scavenging activity of biological samples. The automated method is based on the known reaction of stable 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH) with antioxidants in organic or aqueous-organic media resulting in bleaching of DPPH due to its "quenching" by the interaction with the analytes. The decrease of the absorbance of DPPH (compared to blank experiment carried out with water-ethanol 1:1 instead of the test solution) measured at 525 nm is related to concentration of an antioxidant in the test solution. With the optimised SIA procedure it is possible to detect down to micromolar concentrations of model antioxidants such as ascorbic acid, caffeic acid, (+)-catechin, (-)-epicatechin and rutin and to evaluate the concentration of these antioxidants in the micromolar to millimolar range. The sample throughput is 45 h(-1). Thanks to its rapidity and sensitivity, the proposed SIA method is suitable for performing routine screening tests for the presence of various antioxidants in large series of lyophilised herbal or mushroom extracts (the amount of sample needed for the analysis is several milligrams).

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Section: Resultssupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rapid automated assay of anti-oxidation/radical-scavenging activity of natural substances by sequential injection technique (SIA) using spectrophotometric detection

et al. 2004

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“…The radical-scavenging activity of the extracts was determined using diphenyl picryl hydrazyl radical (DPPH) (Aldrich, Milwakee, WI, USA) according to Schmeda-Hirschmann et al 17 Ethanolic solution of peanut skins extract (5 µl; 300 µg ml −1 ) was added to a 1.5 ml ethanolic solution of DPPH radical (20 µg ml −1 ) to give a final extract concentration of 1 µg ml −1 . The mixture was shaken vigorously and left for 5 min.…”

Section: Radical-scavenging Activitymentioning

confidence: 99%

Optimization of extraction of phenolic antioxidants from peanut skins

2004

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This work was conducted to optimize the extraction conditions for the best recovery of antioxidant compounds from peanut skins. The extracts from the peanut skins were obtained by different extraction methods. The extraction conditions were: different ethanol proportions as the solvent (0, 30, 50, 70 and 96% v/v in distilled water), different peanut skin particle sizes (0-1, 1-2 and 2-10 mm and noncrushed skins), different proportions of solvent/skins (20, 30, 40, 50 and 60 ml g −1 ), different extraction times (by maceration and shaking) and different numbers of extractions. The different extracts obtained under different extraction conditions were compared with special regard to yield, total phenolic compounds and radical scavenging activity. The results showed that the best delivery of phenolic compounds was reached using 70% ethanol, non-crushed peanut skins, ratio of solvent/solid of 20 ml g −1 , at 10 min shaking and three extractions. The maximum yield of 0.118 g g −1 was recorded for phenolic compounds when extracted at the optimum conditions.

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“…Samples of G. gargal basidiomes were analyzed in respect to the nutritional content and biological activity [1]. This study showed a moderate effect in the DNA binding assay and also a strong hypotensive response in rats caused by G. gargal basidiomes collected in Valdivia (Chile).…”

Section: Commentarymentioning

confidence: 95%

Grifola gargal and G. sordulenta, Two Wild Edible Mushrooms with Perspectives as Biotech Products

Postemsky¹

2015

Fermentol Techno

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Grifola gargal Singer and Grifola sordulenta Mont. (Singer) are two edible and medicinal mushroom species which occur in the AndinoPatagonian forests of Argentina and Chile. These woods that mainly consist of Nothofagus spp. are under increasing pressure with the consequent need of finding alternative sources for these mushrooms rather than gathering them. The main interest in these species is their culinary properties, which include a characteristic almond flavor present in the basidiomes, mycelium from submerged culture and solid-state fermented substrates, as well as the closely related Grifola frondosa, a well-known medicinal mushroom.In recent years G. gargal and G. sordulenta have been studied for both their medicinal properties and culture conditions by researchers from Argentina, Chile, Japan and USA. However, neither industrial application nor commercial production has been undertaken. In this situation, a relevant role for fermentation technologies to develop possible future products from these wild-grown mushrooms is emphasized. Indeed, biotransformed wheat grains obtained through solid-state fermentation showed antioxidant and antigenotoxic activities which were comparable with those recorded from mycelium and basidiomes. The article comments on the up-to-date findings and state of research of these novel species, emphasizing some future research topics.

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Proximate composition and biological activity of food plants gathered by chilean Amerindians

Cited by 48 publications

References 24 publications

Rapid automated assay of anti-oxidation/radical-scavenging activity of natural substances by sequential injection technique (SIA) using spectrophotometric detection

Rapid automated assay of anti-oxidation/radical-scavenging activity of natural substances by sequential injection technique (SIA) using spectrophotometric detection

Optimization of extraction of phenolic antioxidants from peanut skins

Grifola gargal and G. sordulenta, Two Wild Edible Mushrooms with Perspectives as Biotech Products

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