; +2348064398527 1.1 INTRODUCTION Catfishes (Family: Clariidae) are a large group of predominantly freshwater fishes of high economic importance in many countries of the world especially African and Asian continents (Olaniyi and Omitogun, 2014); and also serve mainly as food in many homes and hotels (Omitogun et al., 2012). They also occur in the large rivers such as the Amazon and Zaire (Bruton, 1988). The family Clariidae is divided into two genuses, namely Clarias and Heterobranchus. Madu et al. (1999) reported that two species of Clarias are most popular for fish farming in Nigeria. These are C. anguillaris and C. gariepinus similarly, only two species of Heterobranchus (H. bidorsalis and H. longifilis) are commonly available (Madu et al., 1999). Recently, there has been an increase in the farming of Catfishes especially Clarias and Heterobranchus species and their hybrids in Nigeria based on their growth performance, short generation interval, and consumer preference or demand, due to their good taste, good flavour, among others (Agbo, 2015; Olaniyi et al., 2017). Consequently, there is a high demand for these catfish seeds by fish farmers for stocking (Olaniyi et al., 2017). Clarias has a single rayed dorsal fin extending almost to the tail and four pairs of barbels on their flattened, strongly depressed head (Reed et al., 1967). The rayed dorsal fin is continuous, extending to the base of the caudal peduncle. C. gariepinus (Fig. 1) has vomer teeth located at the upper jaw which are mostly conical or granular-sub-conical forming a ABSTRACT Catfishes are freshwater fishes of high economic importance in many countries of the world due to their rich source of lipids, protein and other vital nutrients. The present investigation characterizes lipids from two species; Heterobranchus longifillis and Clarias gariepinus of catfishes from Otuocha River, Anambra State, Nigeria. The oils were extracted using soxhlet apparatus and the oil were qualitatively characterized using the standard methods of AOAC and AOCS. The fatty acid profile of the oils were determined using gas chromatography. The results showed that H. longifillis had the highest oil composition of 15.33±0.28 % per 100 g DW followed by C. gariepinus (14.83±1.61 % per 100 g DW). The peroxide values of oil extracted from H. longifilis was 5.33±1.51 meq/kg and C. gariepinus, 3.34±1.51 meq/kg; the acid values for oil extracted from H. longifilis was 2.03 ± 0.23 mgKOH/g and C. gariepinus, 1.89±0.13 mgKOH/g. p-anisidine values of 15.38±0.20 and 13.57±0.0.28 were obtained for H. longifilis and C. gariepinus oils respectively while the TOTOX values are 6.12 ± 0.51 and 20.99±1.08 respectively. Fatty acid profile analysis showed that lauric (12:0), miristric (14:0), palmitic (16:0), stearic (18:0), oleic (18:1), linoleic (18:9,12), and linolenic acids (18:6,9,12) were dominant in the oils of these fishes. This study has revealed that the qualitative parameters of the extracted oils are within the recommended range by FAO. By having the lowest n-6 PUFA and best qual...