Protuboxepins A and B and Protubonines A and B from the Marine-Derived Fungus Aspergillus sp. SF-5044

Abstract

Two new oxepin-containing (1 and 2) and two diketopiperazine-type alkaloids (3 and 4) have been isolated from an EtOAc extract of the marine-derived fungus Aspergillus sp. SF-5044. The structures of these metabolites were determined through analysis of NMR and MS data, along with Marfey's method. Compound 1 showed weak growth inhibitory activity against a small panel of cell lines.

Measurement of Lipid Droplet Accumulation Kinetics in Chlamydomonas reinhardtii Using Seoul-Fluor

“…To determine cell viability, we used EZ-Cytox that enhanced sensitivity of MTT assays and has been widely used in various organisms [29,31,32]. SF44 staining condition was evaluated with EZ-Cytox assays over 2 days (Figure 2).…”

Cristazine, a New Cytotoxic Dioxopiperazine Alkaloid from the Mudflat-Sediment-Derived Fungus <i>Chaetomium cristatum</i>

“…1,2) Among the marine fungi, those associated with marine sediments are a particularly promising source of novel natural products because they inhabit a special ecological niche. 2,3) As part of a program to explore the bioactive metabolites produced by fungi isolated from marine habitats, we directed our efforts towards the microorganisms associated with the sediments of marine mudflats, 4) and isolated the fungal strain Chaetomium cristatum. This paper describes the isolation and structural characterization of a new dioxopiperazine alkaloid, cristazine (1), and the known chetomin (2), 5,6) neoechinulin A (3), 7,8) and golmaenone (4) 7) from C. cristatum.…”

Introduction to the Analysis of Bioactive Compounds in Marine Samples

“…These metabolites have been subdivided by Kumar et al [49] into seven subclass: pyridoacrine alkaloids (tetracyclic, pentacyclic, hexacyclic, heptacyclic, and octacyclic alkaloids), indole alkaloid (bisindole, indolocarbazole, ergoline, peptidoindole, b-carboline, and trisindole alkaloids), pyrrole alkaloid (bromopyrroles, pyrroloquinones, pryrrodoquinoline alkaloids, pyrrodoacridines), isoquinoline alkaloids, gadinine alkaloids, aminoimidazole alkaloids, and sterol alkaloids. Alkaloids produced by endophytic fungi and algae were reviewed by Zhang et al [50] and Güven et al [51] and have several bioactivities such as antiviral, antibacterial, anti-inflammatory, antimalarial, antioxidant, and anticancer. The caerulomycin F (Table 1.2) has been extracted by Fu et al [38] from the bacteria Actinoalloteichus cyanogriseus, and it has shown cytotoxic activity.…”