“…Bilateral tissue punches of the SCN located approximately from −0.92 mm from bregma (12 gauge), prefrontal cortex (PFC) located approximately 4.2 mm from bregma (16 gauge), VTA located approximately −5.2 mm from bregma (16 gauge) and NAc located approximately 2.2 mm from bregma (16 gauge for two punches) were then taken. Tissue punches were homogenized (10-15 × 3, 5 seconds interval) with an electrical disperser (Wiggenhauser, Sdn Bhd) after 30 minutes in radioimmunoprecipitation assay (RIPA) lysis buffer [Beyotime Biotechnology, Wuhan, China (Wei et al 2011); 20 mM Tris (pH 7.5), 150 mM NaCl, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM ethylenediaminetetraacetic acid, 1% Na3VO4, 0.5 μg/ml leupeptin and 1 mM phenylmethanesulfonyl fluoride]. Tissue homogenates GSK-3β in diurnal variation of cocaine-induced CPP 997…”