Prototype of an in vitro model of the microcirculation

Shevkoplyas, Sergey S.; Gifford, Sean C.; Yoshida, Tatsuro; Bitensky, Mark W.

doi:10.1016/s0026-2862(02)00034-1

Cited by 73 publications

(75 citation statements)

References 12 publications

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“…The UTS and E of the blood vessels has values from 0.4 MPa to 1.4 MPa (Holzapfel et al 2005) and ∼2.5 MPa (Steiger et al 1989) respectively. The UTS and E is PDMS formulation dependent, by adjusting the ratio of the crosslinker (curing agent) and also the thickness of the wall, PDMS may be able to mimic more closely the geometric and structural properties of in vivo microvessels; 3. easy to replicate complex microvascular networks with submicron fidelity (McDonald et al 2002, Shevkoplyas et al 2003, Shin et al 2004, Lima 2007); 4. due to its spontaneous adhesion onto glass substrates, PDMS sealing does not need any complex bonding technique. Hence, PDMS microchannels besides offering optimum optical access for confocal systems, they can be reused several times because of its reversible bonding.…”

Section: Pdms Versus Glass Microchannelsmentioning

confidence: 99%

“…In addition to providing the ability to investigate a variety of phenomena in the microcirculation (Takayama et al 1999, Fujii 2002, Shevkoplyas et al 2003, this kind of microfluidic device may be useful as a "lab-on-a-chip," a clinical diagnostic instrument. However, until now, precise measurements of blood flow behavior through this kind of microchannel has not been performed.…”

Section: Ongoing Developmentmentioning

confidence: 99%

“…By using a soft lithography technique it is possible to generate extremely precise, reproducible and versatile rectangular microchannels. Although, rectangular microchannels may not be the best models to simulate in vivo microvessels geometry, many phenomena of blood flow behavior through this kind of microchannels exhibits certain features characteristic with those investigated in living microvessels (Beebe et al 2002, Shevkoplyas et al 2003, ,Toner and Irimia 2005, Faivre et al 2006. Furthermore, experiments with this kind of microchannels enable not only precise control of experimental parameters but also obtain more accurate measurements.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system

Lima

Wada

Tanaka

et al. 2007

Biomed Microdevices

173

132

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Section: Pdms Versus Glass Microchannelsmentioning

confidence: 99%

Section: Ongoing Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system

Lima

Wada

Tanaka

et al. 2007

Biomed Microdevices

173

132

View full text Add to dashboard Cite

show abstract

“…[285][286][287] Bitensky's group 285,286 used an in vitro model of the blood microcirculatory system consisting of a network of PDMS microchannels patterned after the dimensions and architecture of the mammalian microcirculation. The device was cast in PDMS, and the channels were rendered hydrophilic to facilitate flow by immobilizing a PEG silane.…”

Section: Vh Blood Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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“…For blood rheology, many researchers have attempted to study the rheological properties of RBCs with silicon microchannels. Shevkoplyas et al [6] designed and fabricated a microfluidic chip with a network of microchannels, and the flow of red and white blood cells in this network was visualized by high-speed camera. Parachute and bullet shapes, as well as rouleaux formation, of red blood cells were observed in their experiment.…”

Section: Introductionmentioning

confidence: 99%

Visualization study of motion and deformation of red blood cells in a microchannel with straight, divergent and convergent sections

2011

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The size of red blood cells (RBC) is on the same order as the diameter of microvascular vessels. Therefore, blood should be regarded as a two-phase flow system of RBCs suspended in plasma rather than a continuous medium of microcirculation. It is of great physiological and pathological significance to investigate the effects of deformation and aggregation of RBCs on microcirculation. In this study, a visualization experiment was conducted to study the microcirculatory behavior of RBCs in suspension. Motion and deformation of RBCs in a microfluidic chip with straight, divergent, and convergent microchannel sections have been captured by microscope and high-speed camera. Meanwhile, deformation and movement of RBCs were investigated under different viscosity, hematocrit, and flow rate in this system. For low velocity and viscosity, RBCs behaved in their normal biconcave disc shape and their motion was found as a flipping motion: they not only deformed their shapes along the flow direction, but also rolled and rotated themselves. RBCs were also found to aggregate, forming rouleaux at very low flow rate and viscosity. However, for high velocity and viscosity, RBCs deformed obviously under the shear stress. They elongated along the flow direction and performed a tank-treading motion.

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Prototype of an in vitro model of the microcirculation

Cited by 73 publications

References 12 publications

In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system

In vitro blood flow in a rectangular PDMS microchannel: experimental observations using a confocal micro-PIV system

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Visualization study of motion and deformation of red blood cells in a microchannel with straight, divergent and convergent sections

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