Protoporphyrin IX production and its photodynamic effects on glioma cells, neuroblastoma cells and normal cerebellar granule cells in vitro with 5-aminolevulinic acid and its hexylester

Wu, Siqi; Ren, Qiuyue; Zhou, Miou; Peng, Qian; Chen, J.Y.

doi:10.1016/s0304-3835(03)00271-4

Cited by 45 publications

(28 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This intermediate of porphyrin biosynthesis is converted to protoporphyrin IX (PP IX) in most cell types. Quite selective accumulation of 5-ALA-induced PP IX has been demonstrated for malignant glioma cells in vitro (23,24) and in vivo (25,26) as well as for many other tumour cells. In addition, due to the photosensitizing properties of PP IX, photodynamic treatment of malignant glioma might be considered as a promising additional therapeutic option, which is currently investigated in vitro (27,28) and in vivo (29)(30)(31)(32).…”

Section: Introductionmentioning

confidence: 98%

Photodynamic therapy of malignant glioma with hypericin: Comprehensive in vitro study in human glioblastoma cell lines

Ritz¹,

Wein²,

Dietz³

et al. 2007

Int J Oncol

View full text Add to dashboard Cite

Abstract. The poor prognosis of patients suffering from malignant glioma requires further efforts. Photodynamic therapy (PDT) might be a therapeutic option to increase surgical radicality. Hypericin (HY) exhibit high phototoxicity to malignant cells and accumulates to a higher extent in glioblastoma cells as compared to neurons. Therefore, the impact of various experimental parameters on cytotoxicity, intracellular accumulation and phototoxicity of HY was quantitatively assessed in the three human glioblastoma cell lines U373 MG, LN229 and T98G. Additionally, intracellular location of HY was studied with fluorescence microscopic techniques. For all three cell lines, no cytotoxicity was found for incubation concentrations up to 5 μM. For short-time incubation (2 h), maximum HY fluorescence was achieved at an incubation concentration of about 5 μM. However, uptake kinetics of HY was dependent on its incubation concentration. Moreover, increase in HY fluorescence was negligible at 4˚C, which strongly indicates that the compound is taken up by an energy-dependent process. HY exhibited high phototoxicity (at 595 nm) in all three cell lines with ID 50 -values ranging from 0.15 J/cm 2 to 0.22 J/cm 2 , but sensitivity decreased in the order U373 MG > LN229 > T98G. However, assessment of phototoxicity at different wavelengths revealed that highest cell inactivation was achieved at 600 nm. Fluorescence microscopy showed that HY fluorescence arose predominantly from the perinuclear region and the nuclear membrane. Fluorescence pattern of HY was significantly different from those observed for organelle markers staining lysosomes or mitochondria. Location of HY in the plasma membrane was proven by total internal reflection fluorescence microscopy. Thus, the present study demonstrates that glioblastoma cells can be effectively inactivated by HY-PDT after short-time incubation and exposure to low light doses. These results obtained in cell culture are encouraging and justify further evaluation HY-PDT for the treatment of malignant glioma in animal experiments.

show abstract

Section: Introductionmentioning

confidence: 98%

Photodynamic therapy of malignant glioma with hypericin: Comprehensive in vitro study in human glioblastoma cell lines

Ritz¹,

Wein²,

Dietz³

et al. 2007

Int J Oncol

View full text Add to dashboard Cite

show abstract

“…Following the exogenous administration of ALA, tumor cells selectively accumulate photosensitizer protoporphyrin IX (PPIX), which, under suitable irradiation, generates cytotoxic species leading to cellular damage. Therefore, ALA is widely used as a topical drug in PDT [5,6].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy

Zhang¹,

Wei²,

Yin³

2009

ABBS

View full text Add to dashboard Cite

Mangostin (MAG), a kind of xanthone widely used in diet and medicine, has antioxidant, anti-inflammatory, antimicrobial, and anticancer activities. On account of its antioxidant activity, MAG might protect cancer cells from free radical damage in photodynamic therapy (PDT) during which reactive oxygen species production was stimulated leading to irreversible tumor cell injury. In this study, the antioxidant activity of MAG was investigated and the influence of MAG on K562 cells in 5-aminolevulinic acid (ALA)-based PDT is demonstrated. The results showed that MAG could scavenge hydroxyl radical, superoxide anion, and hydrogen peroxide and inhibit the formation of malondialdehyde (MDA), but increase the amounts of singlet oxygen in cell-free systems. MAG inhibits cell proliferation and enhances cell apoptosis, lipid peroxidation, and DNA damage in ALA-PDT on K562 cells. NaN 3 , a singlet oxygen quencher, suppresses the MAG-induced cell apoptosis, lipid peroxidation, and DNA damage. In conclusion, MAG enhances the PDT-induced cytotoxicity in K562 cells and singlet oxygen was involved in this process. These results implied that the effect of antioxidants on PDT might be determined by its sensitization ability to singlet oxygen.

show abstract

“…Nevertheless, the RG2 glioma cells are much less used in research than the C6 glioma cells. In particular, whereas several papers described reactions of the C6 cells in vivo to ALA-mediated PDT [2,7,17] and SDT [8,9,14], we were unable to find any report on experiments with the RG2 cells and either SDT or PDT.…”

Section: Discussionmentioning

confidence: 61%

5-Aminolevulinic acid-mediated sonosensitization of rat RG2 glioma cells in vitro

Bilmin¹,

Kujawska²,

Secomski³

et al. 2016

View full text Add to dashboard Cite

show abstract

Protoporphyrin IX production and its photodynamic effects on glioma cells, neuroblastoma cells and normal cerebellar granule cells in vitro with 5-aminolevulinic acid and its hexylester

Cited by 45 publications

References 21 publications

Photodynamic therapy of malignant glioma with hypericin: Comprehensive in vitro study in human glioblastoma cell lines

Photodynamic therapy of malignant glioma with hypericin: Comprehensive in vitro study in human glioblastoma cell lines

Antioxidant activity of mangostin in cell-free system and its effect on K562 leukemia cell line in photodynamic therapy

5-Aminolevulinic acid-mediated sonosensitization of rat RG2 glioma cells in vitro

Contact Info

Product

Resources

About