Protoporphyrin IX (PpIX) loaded PLGA nanoparticles for topical Photodynamic Therapy of melanoma cells

Silva, Darlan Barbosa da; Silva, Carolina Loureiro da; Davanzo, Nathalia Nossi; Souza, Rodrigo da Silva; Correa, Rodrigo; Tedesco, Antônio Cláudio; Pierre, Maria Bernadete Riemma

doi:10.1016/j.pdpdt.2021.102317

Cited by 13 publications

(13 citation statements)

References 52 publications

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“…This cell line is a stable malignant melanoma tumour cell line that can be studied in vivo, the tumour cells being injected into organism, thus developing melanoma in a animal model [ 118 ]. Thus, Da Silva et al showed in [ 119 ] that PpIX ( Table 2 ) encapsulation in poly (D, L lactic-co-glycolic acid) (PLGA) NPs improved the PDT effect on the melanoma B16-F10 cells at a light dose (1500 mJ/cm 2 at 630 nm) with only 21.7% viable cells after exposure. Very good phototoxicity values were observed compared with PpIX, but no cytotoxicity was observed in the dark.…”

Section: Porphyrinsmentioning

confidence: 99%

Photosensitizers-Loaded Nanocarriers for Enhancement of Photodynamic Therapy in Melanoma Treatment

et al. 2023

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Malignant melanoma poses a significant global health burden. It is the most aggressive and lethal form of skin cancer, attributed to various risk factors such as UV radiation exposure, genetic modifications, chemical carcinogens, immunosuppression, and fair complexion. Photodynamic therapy is a promising minimally invasive treatment that uses light to activate a photosensitizer, resulting in the formation of reactive oxygen species, which ultimately promote cell death. When selecting photosensitizers for melanoma photodynamic therapy, the presence of melanin should be considered. Melanin absorbs visible radiation similar to most photosensitizers and has antioxidant properties, which undermines the reactive species generated in photodynamic therapy processes. These characteristics have led to further research for new photosensitizing platforms to ensure better treatment results. The development of photosensitizers has advanced with the use of nanotechnology, which plays a crucial role in enhancing solubility, optical absorption, and tumour targeting. This paper reviews the current approaches (that use the synergistic effect of different photosensitizers, nanocarriers, chemotherapeutic agents) in the photodynamic therapy of melanoma.

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Section: Porphyrinsmentioning

confidence: 99%

Photosensitizers-Loaded Nanocarriers for Enhancement of Photodynamic Therapy in Melanoma Treatment

et al. 2023

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“…A schematic diagram of the mechanism is shown in Figure 6. At present, PDT is mainly used to treat infections [32] and tumors [33,34]. In orthopedic surgery, PDT has been used in the treatment of osteosarcoma [35] and prosthetic joint infections [36], and its effect on the treatment of soft tissue injury has been studied in animal experiments [37].…”

Section: Quaternary Ammonium Compoundsmentioning

confidence: 99%

Antibacterial and Anti-Inflammatory Coating Materials for Orthopedic Implants: A Review

Tan

Chirume

et al. 2021

Coatings

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Orthopedic implant failure is the most common complication of orthopedic surgery, causing serious trauma and resulting in a tremendous economic burden for patients. There are many reasons for implant failure, among which peri-implant infection (or implant-related infection) and aseptic loosening are the most important. At present, orthopedic doctors have many methods to treat these complications, such as revision surgery, which have shown good results. However, if peri-implant infection can be prevented, this will bring about significant social benefits. Many studies have focused on adding antibacterial substances to the implant coating, and with a deeper understanding of the mechanism of implant failure, adding such substances by different modification methods has become a research hot spot. This review aims to summarize the antibacterial and anti-inflammatory substances that can be used as coating materials in orthopedic implants and to provide a reference for the prevention and treatment of implant failure caused by implant-related infection and excessive inflammation.

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“…Photodynamic therapy (PDT) and photothermal therapy (PTT) are two kinds of highly effective and precise tumor treatment. , PDT has attracted tremendous attention for its high localized efficiency and the little damage it does to surrounding normal tissue . PDT includes three essential components: excitation light, photosensitizers (PSs), and reactive oxygen species (ROS). , Studies showed that during the PDT process, the PS could generate high ROS under reasonable excitation light, which could kill tumor cells. − Among the commonly used PSs, PpIX is favorable for its strong photosensitive activity, low toxicity, and fast metabolism. , Chudal et al designed and synthesized a nanoplatform (PpIX-Lipo-MnO 2 ) based on PpIX. The PDT efficiency of the PpIX-Lipo-MnO 2 nanoparticles (NPs) could be enhanced greatly by converting common H 2 O 2 into oxygen.…”

Section: Introductionmentioning

confidence: 99%

“…12−14 Among the commonly used PSs, PpIX is favorable for its strong photosensitive activity, low toxicity, and fast metabolism. 15,16 Chudal et al 17 Another ideal tumor treatment is PTT, which is noninvasive, repeatable, and safe. 18,19 The photothermal agent could convert light energy into hyperthermia under laser irradiation and then induce the ablation of tumor cells.…”

Section: Introductionmentioning

confidence: 99%

“… 12 − 14 Among the commonly used PSs, PpIX is favorable for its strong photosensitive activity, low toxicity, and fast metabolism. 15 , 16 Chudal et al 17 designed and synthesized a nanoplatform (PpIX-Lipo-MnO 2 ) based on PpIX. The PDT efficiency of the PpIX-Lipo-MnO 2 nanoparticles (NPs) could be enhanced greatly by converting common H 2 O 2 into oxygen.…”

Section: Introductionmentioning

confidence: 99%

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PpIX/IR-820 Dual-Modal Therapeutic Agents for Enhanced PDT/PTT Synergistic Therapy in Cervical Cancer

et al. 2022

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High treatment accuracy is the key to efficient cancer treatment. Photodynamic therapy (PDT) and photothermal therapy (PTT) are two kinds of popular, precise treatment methods. The combination of photodynamic and photothermal therapy (PDT/PTT) can greatly enhance the precise therapeutic efficacy. In this work, protoporphyrin IX (PpIX) was selected as the PDT agent (photosensitizer), and new indocyanine green (IR-820) was selected as the PTT agent. Further, the two kinds of theranostic agents were encapsulated by biological-membrane-compatible liposomes to form PpIX-IR-820@Lipo nanoparticles (NPs), a new kind of PDT/PTT agent. The PpIX-IR-820@Lipo NPs exhibited good water solubility, a spherical shape, and high fluorescence peak emission in the near-infrared spectral region (700–900 nm, NIR). The cellular toxicity of PpIX-IR-820@Lipo NPs for human cervical cancer cells (HeLa) and human cervical epithelial cells (H8) was detected by the CCK-8 method, and low cytotoxicity was observed for the PpIX-IR-820@Lipo NPs. Then, the excellent cellular uptake of PpIX-IR-820@Lipo NPs was confirmed by laser scanning confocal microscopy. Moreover, the PDT/PTT property of PpIX-IR-820@Lipo NPs was illustrated via 2′,7′-dichlorofluorescin diacetate (DCFH-DA) and annexin V-fluorescein isothiocyanate (annexin V-FITC), as indicator probes. The PDT/PTT synergistic efficiency of PpIX-IR-820@Lipo NPs on HeLa cells was verified, exhibiting a high efficiency of 70.5%. Thus, the novel theranostic PpIX-IR-820@Lipo NPs can be used as a promising PDT/PTT synergistic theranostic nanoplatform in future cervical cancer treatment.

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Protoporphyrin IX (PpIX) loaded PLGA nanoparticles for topical Photodynamic Therapy of melanoma cells

Cited by 13 publications

References 52 publications

Photosensitizers-Loaded Nanocarriers for Enhancement of Photodynamic Therapy in Melanoma Treatment

Photosensitizers-Loaded Nanocarriers for Enhancement of Photodynamic Therapy in Melanoma Treatment

Antibacterial and Anti-Inflammatory Coating Materials for Orthopedic Implants: A Review

PpIX/IR-820 Dual-Modal Therapeutic Agents for Enhanced PDT/PTT Synergistic Therapy in Cervical Cancer

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