Protoplasts of Eukaryotic Algae

Berliner, Martha D.

doi:10.1016/s0074-7696(08)61284-x

Cited by 23 publications

(9 citation statements)

References 32 publications

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“…has been generated by protoplast production procedures (Berliner, 1981). Ohiwa (1978) measured the internal osmotic pressure and determined 0.27 M sucrose-equivalent in Zygnema and 0.38 M sucrose-equivalent in Spirogyra , when attempting to generate fusion products of Spirogyra and Zygnema protoplasts.…”

Section: Introductionmentioning

confidence: 99%

Osmotic stress in Arctic and Antarctic strains of the green alga Zygnema (Zygnematales, Streptophyta): Effects on photosynthesis and ultrastructure

Kaplan

Lewis

Herburger

et al. 2013

Micron

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Highlights► We investigate the osmotic potential of four polar green algal strains. ► We examine effects of osmotic stress on ultrastructure and photosynthesis. ► One investigated strain belongs to a different phylogenetic lineage. ► Arctic strains show higher osmotic values (more negative osmotic potentials). ► Physiological performance and ultrastructure are affected by osmotic stress.

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Section: Introductionmentioning

confidence: 99%

Osmotic stress in Arctic and Antarctic strains of the green alga Zygnema (Zygnematales, Streptophyta): Effects on photosynthesis and ultrastructure

Kaplan

Lewis

Herburger

et al. 2013

Micron

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“…The chemical composition of algal cell walls differs so much from that of vascular plants that wall-digesting enzymes used for obtaining protoplasts of vascular plants do not work on algal tissues. Berliner (1981Berliner ( , 1983, Cheney (1986), Polne-Fuller et al (1986) and Saga et al (1986) have summarized much of the work to date on protoplast production and regeneration in eukaryotic algae. Only a small number of seaweeds have yielded protoplasts which could regenerate multicellular calli or thalli (e.g.…”

Section: Introductionmentioning

confidence: 99%

Protoplast isolation and regeneration in the marine red alga Porphyra nereocystis

Waaland

Dickson

Watson

1990

Planta

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We have developed a method for isolating viable protoplasts from the blade phase of the epiphytic marine red alga Porphyra nereocystis Anderson, using a two-step enzymatic digestion with commercially available enzymes. The first step uses papain, the second step uses abalone acetone powder. The method is rapid and gives a high yield of viable protoplasts. In liquid culture in enriched seawater medium, the protoplasts can undergo regeneration along three pathways: they directly form filaments resembling the conchocelis phase of Porphyra; they form calli with relatively thick-walled, pigmented cells; and they indirectly form blades from the edges of these calli. Porphyra nereocystis protoplasts also may serve as an alternative propagation method in aquaculture and be useful for studies of cell-wall formation, cell division, and thallus differentiation. They may also be used in somatic selection, somatic hybridization and gene-transfection experiments.

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“…Previous reports of single cell isolation from seaweeds have cited the necessity of using axenic tissue as bacteria thrive on the enzyme-osmoticum solution (Berliner, 1981;Zhang, 1983;Polne-Fuller & Gibor, 1984Polne-Fuller et al, 1984;Polne-Fuller et al, 1986;Chen, 1986;Wue-wu & Gordon, 1987). However, successful techniques have been developed for isolation of single cells from unialgal (but not bacteria free) tissue which are more rapid and still result in high levels of viability (Sage, 1984;Saga et al, 1987).…”

Section: Resultsmentioning

confidence: 99%

Strain selection and cell isolation of Ulvaria oxysperma (Kuetz.) Bliding (Chlorophyta) for net cultivation

Kapraun

Sherman

1989

Hydrobiologia

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North Carolina and Bermuda isolates of the green seaweed Ulvaria oxyspenna (Kuetz.) Bliding were grown in cross-gradient light-temperature culture to determine the effects of varied photon fluence rates, temperature and photoperiod on their growth and reproduction. Results indicated significant geneticbased variation between the two populations.Spheroplast were obtained from the vegetative thallus of the North Carolina isolate following enzymatic digestion with abalone gut extract and maceration. Single cell isolates developed new cell walls in 2-3 days and attached to nylon net substrate. Cultured isolates regenerated into juvenile plants with normal development and morphology.Results are discussed in terms of the suitability of this economically important species for land-based mariculture.

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Protoplasts of Eukaryotic Algae

Cited by 23 publications

References 32 publications

Osmotic stress in Arctic and Antarctic strains of the green alga Zygnema (Zygnematales, Streptophyta): Effects on photosynthesis and ultrastructure

Osmotic stress in Arctic and Antarctic strains of the green alga Zygnema (Zygnematales, Streptophyta): Effects on photosynthesis and ultrastructure

Protoplast isolation and regeneration in the marine red alga Porphyra nereocystis

Strain selection and cell isolation of Ulvaria oxysperma (Kuetz.) Bliding (Chlorophyta) for net cultivation

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