Protoplast plasmalemma fluidity of hardened wheats correlates with frost resistance

Vı́gh, László; Horváth, László; Horváth, Ibolya; Dudits, Dénes; Farkas, Tibor

doi:10.1016/0014-5793(79)80393-2

Cited by 48 publications

(15 citation statements)

References 16 publications

(3 reference statements)

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“…Taking into account this criterion and using protoplasts or plasma membrane-purified fractions, some reports emphasize the potential role of the plasma membrane in cold tolerance (23,30,33). Other data suggest that thylakoidal membranes are very sensitive to freezing (18).…”

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confidence: 99%

Lateral and Rotational Mobilities of Lipids in Specific Cellular Membranes of Eucalyptus gunnii Cultivars Exhibiting Different Freezing Tolerance

Leborgne¹,

Dupou-Cézanne²,

Teulières³

et al. 1992

Plant Physiol.

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Two cell lines of Eucalyptus gunnii have been shown to keep their differential frost tolerance at the cellular level after longterm culture. They have been used to investigate the fluidity of specific cell membranes in relation with frost tolerance. Protoplasts and isolated vacuoles were obtained from both cell lines. In addition, purified plasma membrane and tonoplast (the vacuolar membrane) were separated from a crude microsomal fraction through free-flow electrophoresis. The lateral and rotational mobilities of lipids in these different membranes were studied by two biophysical techniques: fluorescence recovery after photobleaching (FRAP) and fluorescence polarization. After labeling the vacuoles isolated from the frost-sensitive cells with 1-oleoyl-2-(7-nitro-2,1,3-benzoxadiazol-4-yl)aminocaproyl phosphatidylcholine, a single mobile component was observed with a diffusion coefficient of 2.4 x 10-9 cm2 s-i and a mobile fraction close to 100% at a temperature of 23°C. When using isolated vacuoles from the frost tolerant line, a higher lateral diffusion of tonoplast lipids was found with a diffusion coefficient of 3.2 x 10'9 cm2 s-1, still with a mobile fraction close to 100%. No convincing data were obtained when performing fluorescence recovery after photobleaching experiments on protoplasts. Fluorescence polarization experiments confirmed the differential behavior of the two cell lines for tonoplast and also for plasma membrane. In addition, they showed that intrinsically tonoplast exhibited a higher fluidity than plasma membrane. Our results provide the first information on the fluidity of tonoplast and on the compared properties of two important plant membranestonoplast and plasma membrane-through the use of two complementary biophysical approaches. In addition, they suggest there is a correlation between membrane fluidity and cold tolerance. The potential interest of plant vacuole as a natural model system in membrane studies is emphasized.

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confidence: 99%

Lateral and Rotational Mobilities of Lipids in Specific Cellular Membranes of Eucalyptus gunnii Cultivars Exhibiting Different Freezing Tolerance

Leborgne¹,

Dupou-Cézanne²,

Teulières³

et al. 1992

Plant Physiol.

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“…It was shown decades ago, that fluidity, organisation and phase behaviour of membranes are key and strictly controlled factors in the processes of thermally induced adaptation and lethality, in both prokaryotic [29][30][31] and eukaryotic cells [32][33][34][35][36]. Our early findings, achieved with prokaryotic models firstly revealed that membranes can act as thermosensors, and there exists a feed-back membrane fluidity control of certain stress defending genes, such as fatty acid desaturases in the cold [37].…”

Section: Membranes Are Key Determinants Of Cellular Stress Adaptationmentioning

confidence: 90%

Membrane fluidity matters: Hyperthermia from the aspects of lipids and membranes

Csoboz

Balogh

Kúsz

et al. 2013

International Journal of Hyperthermia

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Hyperthermia is a promising treatment modality for cancer in combination both with radio-and chemotherapy. In spite of its great therapeutic potential, the underlying molecular mechanisms still remain to be clarified. Due to lipid imbalances and 'membrane defects' most of the tumour cells possess elevated membrane fluidity. However, further increasing membrane fluidity to sensitise to chemo-or radiotherapy could have some other effects. In fact, hyperfluidisation of cell membrane induced by membrane fluidiser initiates a stress response as the heat shock protein response, which may modulate positively or negatively apoptotic cell death. Overviewing some recent findings based on a technology allowing direct imaging of lipid rafts in live cells and lipidomics, novel aspects of the intimate relationship between the 'membrane stress' of tumour cells and the cellular heat shock response will be highlighted. Our findings lend support to both the importance of membrane remodelling and the release of lipid signals initiating stress protein response, which can operate in tandem to control the extent of the ultimate cellular thermosensitivity. Overall, we suggest that the fluidity variable of membranes should be used as an independent factor for predicting the efficacy of combinational cancer therapies.

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“…9, 13). In addition, increased membrane fluidity has also been implicated in the stimulation of ion fluxes and water flow in roots pretreated at low temperatures (4,6,13,17). However, there is little definitive information available regarding the biochemical basis of this adjustment.…”

Section: Discussionmentioning

confidence: 99%

Regulation of K⁺ Influx in Barley

Siddiqi

Memon²,

Glass³

1984

Plant Physiol.

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Influx and accumulation of K' in barley (Hordeum vulgare L. cv Fergus) roots were measured at two temperatures (10°C and 20C) in plants which had been grown with roots and shoots at 20°C (HT plants), with roots and shoots at 10°C (LT plants), and with roots at 10°C and shoots at 20°C (DT plants example, compared to control plants grown at 200C continuously, plants whose roots had been maintained at 8°C demonstrated increased rates of nutrient absorption, accumulation, translocation, and xylem exudation when these processes were measured at 20C.Clarkson and his co-workers (4, 5, 7) have emphasized that the observed responses represent a strategy which compensates for the lower root activity and higher shoot activity associated with the temperature differential. However, similar increases in ion fluxes in response to pretreatment at low temperatures have been demonstrated in a giant alga where differential temperatures were not involved (12,13). It is therefore important to resolve, in higher plants, whether the stimulation of ion fluxes is due to differential (root/shoot) temperature or due to exposure of roots to low temperature per se irrespective ofshoot temperature. This may have important implications as to the source(s) of the signal(s) responsible for the observed adjustments of ion fluxes.Moreover, the extent of the accommodation of ion fluxes may have been considerably underestimated in previous studies. At face value it would appear that roots, maintained at 10°C below shoot temperatures, achieve higher levels of ion accumulation and ion fluxes which are equal to or even slightly higher (on a per unit weight basis) than those of roots where roots and shoots are maintained at the higher temperature. However, it is well documented that ion uptake is negatively correlated with tissue ion concentration (8,10,14

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Protoplast plasmalemma fluidity of hardened wheats correlates with frost resistance

Cited by 48 publications

References 16 publications

Lateral and Rotational Mobilities of Lipids in Specific Cellular Membranes of Eucalyptus gunnii Cultivars Exhibiting Different Freezing Tolerance

Lateral and Rotational Mobilities of Lipids in Specific Cellular Membranes of Eucalyptus gunnii Cultivars Exhibiting Different Freezing Tolerance

Membrane fluidity matters: Hyperthermia from the aspects of lipids and membranes

Regulation of K⁺ Influx in Barley

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Protoplast plasmalemma fluidity of hardened wheats correlates with frost resistance

Cited by 48 publications

References 16 publications

Lateral and Rotational Mobilities of Lipids in Specific Cellular Membranes of Eucalyptus gunnii Cultivars Exhibiting Different Freezing Tolerance

Lateral and Rotational Mobilities of Lipids in Specific Cellular Membranes of Eucalyptus gunnii Cultivars Exhibiting Different Freezing Tolerance

Membrane fluidity matters: Hyperthermia from the aspects of lipids and membranes

Regulation of K+ Influx in Barley

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Regulation of K⁺ Influx in Barley