Protoplast Isolation in the Agar-Producing Seaweed Gracilaria Tikvahiae.

Cheney, Donald P.; Mar, Emily; Meer, John van der

doi:10.1016/b978-0-12-221480-6.50041-4

Cited by 4 publications

(2 citation statements)

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“…Seaweed grazers and pathogens have been used extensively as sources for seaweed cell wall degrading enzymes (Cheney et al ., 1984 ;Liu et al, 1984 ;Polne-Fuller & Gibor, 1984 ;Fujita & Migita, 1985) . These available enzymes were limited in their ability to degrade different algae and different tissue types .…”

Section: Introductionmentioning

confidence: 99%

Digestion of seaweeds by the marine amoeba Trichosphaerium

Polne‐Fuller

Rogerson²,

Amano

et al. 1990

Hydrobiologia

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A crude enzyme preparation from the marine amoeba Trichosphaerium was used to produce protoplasts from Sargassum muticum, Macrocystis pyrifera, Porphyra perforata, and other red and brown marcroalgae . Cortical and medullary protoplasts of Sargassum, which were impossible to obtain using mixtures of previously available enzymes, have now been prepared . Intact inner cortical and medullary protoplasts of Macrocystis, which were not observed in past isolations, were obtained . Improved protoplast yields of as much as 500 fold resulted from feeding the amoebae on specific seaweeds . Cuticles of live Sargassum and Macrocystis were digested easily by the amoebae . However, cuticles of autoclaved Macrocystis and those of Porphyra (fresh or autoclaved) were eaten last . In spite of the absence of identifiable extracellular enzymatic activity in the medium, the amoebae were able to `cut' and consume live fronds and blocks of gelled agars, carrageenans, and alginates .

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Section: Introductionmentioning

confidence: 99%

Digestion of seaweeds by the marine amoeba Trichosphaerium

Polne‐Fuller

Rogerson²,

Amano

et al. 1990

Hydrobiologia

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“…Few species tested produce agar suitable for biomedical purposes . Introduction (Cheney, et al 1983) for agar . If the world's needs for quality agar are to be met in part by Gracilaria, then the species must meet initial criteria before production or mariculture experiments can be considered .…”

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confidence: 99%