2021
DOI: 10.1007/s11033-021-06995-6
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Protoplast isolation and transcriptome analysis of developing xylem in Pinus massoniana (Pinaceae)

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“…Because of the thick cell wall and abundant secondary metabolism, woody plant tissues are hard to digest to release protoplasts (Barros et al ., 2015 ; Butt, 1985 ; Shen et al ., 2022 ). Moreover, the relatively long growth cycles and lack of effective transgenic technologies to validate the spatial and temporal expression profiles of genes have resulted in few cell type‐specific marker genes being detected in the non‐model woody plants.…”
Section: Introductionmentioning
confidence: 99%
“…Because of the thick cell wall and abundant secondary metabolism, woody plant tissues are hard to digest to release protoplasts (Barros et al ., 2015 ; Butt, 1985 ; Shen et al ., 2022 ). Moreover, the relatively long growth cycles and lack of effective transgenic technologies to validate the spatial and temporal expression profiles of genes have resulted in few cell type‐specific marker genes being detected in the non‐model woody plants.…”
Section: Introductionmentioning
confidence: 99%