Protoplast Fusion for the Generation of Unique Plants

Davey, M. R.; Lowe, K. C.; Power, J. B.; Anthony, Paul

doi:10.1002/0471250570.spi096

Cited by 2 publications

(2 citation statements)

References 47 publications

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“…Electrofusion, chem i cal treat ment of proto plasts with PEG or a com bi na tion of these treat ments, re main the most com monly used pro ce dures. Hy brid cells that re sult may regen er ate into plants with bal anced or asym met ric nu clear genomes (Davey et al 2000b). Cell-cell inter ac tions fol low ing fu sion may re sult in chloroplast seg re ga tion, or more rarely, the re tention of a mixed plastid pop u la tion.…”

Section: So Matic and Gametosomatic Hy Bridi Sa Tionmentioning

confidence: 99%

Plant protoplast technology: Current status

et al. 2005

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115 9516334; phone +44 115 9513057 Key words: Proto plasts, ge netic ma nip u la tion, nuclear and organelle trans for ma tion, phys i o log i cal in ves ti ga tions, protoplast-to-plant sys tems, so matic hy bridi sa tion ACTA PHYSIOLOGIAE PLANTARUM Vol. 27. No. 1. 2005: 117-129re view liv ing cell con tents into a hypertonic so lu tion. Several years later, what may now be con sid ered as early 'bio tech nol ogy' ex per i ments em a nated from in ves ti ga tions of Kuster (1909) andMichel (1937). These work ers dem on strated that the plasma membranes of iso lated proto plasts (wall-less 'na ked cells') could be in duced to fuse, per mit ting co alescence of their cytoplasms. How ever, such in ves tiga tions passed vir tu ally un no ticed for many years un til the com mer cial avail abil ity, dur ing the 1960s, of wall de grad ing pectinases and cellulases. Such en zymes, when used ei ther se quen tially or in combi na tion, fa cil i tated the re pro duc ible iso la tion of large, work able pop u la tions (>1 x 10 6 ) of viable protoplasts from primary tissues of a range of plant species.

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Section: So Matic and Gametosomatic Hy Bridi Sa Tionmentioning

confidence: 99%

Plant protoplast technology: Current status

et al. 2005

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“…Procedures for protoplast fusion generally involve exposure of isolated protoplasts to chemical fusion agents, such as polyethylene glycol (PEG), exposure of mixtures of parental protoplasts to an alternating current, followed by high voltage direct current pulses (electrofusion), or a combination of these procedures (Davey et al 2000b(Davey et al , 2005a. Generally, electrofusion is the preferred procedure to fuse banana protoplasts (Matsumoto, Vilarinhos, & Oka 2002) and is the most efficient procedure to generate somatic hybrid plants (Assani et al 2005).…”

Section: Somatic Hybridizationmentioning

confidence: 99%

Genetic Improvement of Banana Using Conventional andIn VitroTechnologies

Sipen

Chubo

King

et al. 2011

Journal of Crop Improvement

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Banana ( Musa spp.) is an important nutrient-rich fruit crop cultivated in the tropics and sub-tropics for local consumption and export. Targets for genetic improvement of banana range from improved fruit quality, yield, disease resistance, tolerance to biotic and abiotic stresses, and the biosynthesis of pharmaceutical compounds. Sterility has limited the success of generating new cultivars by conventional breeding. Tissue culture-based technologies that involve embryo rescue, the generation of somaclonal variation, and gene-transfer procedures are a useful adjunct to sexual hybridization, although considerable effort is required to establish robust protoplast-to-plant systems for somatic hybridization. Transformation involving Agrobacterium and biolisticsmediated gene transfer is feasible, underpinned by shoot regeneration from cultured cells and tissues. Molecular characterization of germplasm will facilitate the selection of material most relevant for incorporation into sexual and somatic genetic-improvement programs.

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Protoplast Fusion for the Generation of Unique Plants

Cited by 2 publications

References 47 publications

Plant protoplast technology: Current status

Plant protoplast technology: Current status

Genetic Improvement of Banana Using Conventional andIn VitroTechnologies

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