Protopine Production by Fumaria Cell Suspension Cultures: Effect of Light

Abstract

Protopine biosynthesis in Fumaria rostellata and Fumaria officinalis cell suspensions was investigated. For the first time, we reported for calli and cell suspensions obtained from F. rostellata and F. officinalis. Callus induction was initiated on a Murashige and Skoog medium, supplemented with sucrose and various concentrations of plant growth regulators: 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The best morphological characteristics, growth behavior, and protopine biosynthesis were observed for two callus lines (5FRL14 and 12FOL1) cultivated under submerged conditions, at low concentration of 2,4-D (0.2 and 0.5 mg/L) and higher concentration of BAP (2.0 and 3.0 mg/L). The maximal yield of protopine was accumulated from cell suspension of F. rostellata (line 5FRL14) cultivated under illumination-49.6 mg/L. Time courses of utilization of sucrose, ammonium, nitrate, and phosphate ions in cultural liquid and acetylcholinesterase inhibitory activity of alkaloid extracts of studied suspensions are also presented.

Production of fumaric acid from Fumaria sp. plant in vitro systems

“…The determined contents were similar (0.26% w/w and 0.88% w/w for F. officinalis and F. rostellata, respectively) ( Table 1). These results provoked our interest to investigate biosynthesis of fumaric acid by previously obtained and selected cell suspensions of F. officinalis and F. rostellata (Georgieva et al 2015) at different culture conditions. Further calli and shoots of both species were also investigated for fumaric acid accumulation.…”

“…A protocol for obtaining calli and suspension cultures of Fumaria rostellata Knaf. and Fumaria officinalis L. was described previously by Georgieva et al (2015). The obtained stable suspension cultures were cultivated in flasks (volume 200 mL) on Murashige and Skoog (MS) medium, supplemented with 30.0 g.L -1 sucrose and 0.2 mg.L -1 and 0.5 mg.L -1 of 2,4dichlorophenoxyacetic acid (2,4-D Sigma, USA), respectively, and 2 mg.L -1 6-benzylamynopurine (BAP, Duchefa) with subculturing period of 10 days.…”

Biotechnological approaches for production of bioactive secondary metabolites in Nigella sativa: an up-to-date review

“…Light in Plant tissue culture can play a very major part, it is a type of physical elicitation, fluctuation of intensity and color of light can produce some sort of stress in plant which may lead to initiate culture and also stimulate the production of phytochemicals. Light has stimulated the production of protopine in suspension cultures of Fumaria, [94] phenolics and flavonoids in callus cultures of Stevia rebaudiana, [95] caffeic acid derivatives in hairy root cultures of Echinacea purpurea, [93] phenolic acids in in vitro cultured Ruta graveolens and Ruta graveolens divaricata [96]. Several reports are available on light stimulated cultures of N. sativa.…”

Mass Propagation of Plant Cells – An Emerging Technology Platform for Sustainable Production of Biopharmaceuticals

“…PCCT is a powerful technology platform for production of bioactive phytochemicals, active cosmetic additives, food supplements and nutraceuticals, which in general utilizes non-genetically modified plant cells, grown under controlled microenvironment in a form of suspended single cells and/or small aggregates. Several pharmaceutically active compounds, including: tropane alkaloids atropine, hyoscyamine and scopolamine [4]; benzylisoquinoline alkaloid protopine [5]; diterpenoids as paclitaxel [6]; triterpenoids as ursolic and oieanolic acids [7,8]; pentacyclic triterpenoid saponins as centellosides [9]; stilbenoids as resveratrol [10]; aryl tretralin lactone podophyllotoxin [11]; phenylethanoid glycosides as verbascoside and leucosceptoside A [12]; etc. have been found to accumulate in plant cell suspensions.…”