Proton transfer reaction mass spectrometry: A green alternative for food volatilome profiling

Mazzucotelli, Maria; Farneti, Brian; Khomenko, Iuliia; Gonzalez-Estanol, Karina; Pedrotti, Michele; Fragasso, Mariagiovanna; Capozzi, Vittorio; Biasioli, Franco

doi:10.1016/j.greeac.2022.100041

Cited by 10 publications

(7 citation statements)

References 117 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results agree with the studies of Aprea et al (2009) ; Cappellin et al (2013) , in which the VOC profile of some raspberry cultivars was evaluated on intact berries with PTR-MS equipped with a quadrupole mass detector. The main advantages of using PTR-MS equipped with a ToF analyzer are the enhanced mass resolution, allowing the separation of many isobaric compounds and the simultaneous monitoring of multiple peaks at the same nominal mass, and the enhanced speed of analysis since the whole VOC spectrum is acquired in a fraction of a second ( Mazzucotelli et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, several VOCs are considered key target molecules to improve the flavour perception of fruit and vegetables and, consequently, their marketability. In addition, the end of the “flavour life”, mainly due to changes in VOC composition, often precedes the end of shelf life as determined by external modifications ( Mazzucotelli et al, 2022 ). Thus, VOCs should be considered a central trait to determine the optimal cultivation and storage strategies, especially for products for which repeated purchasing behavior and willingness to pay are associated with positive eating experience.…”

Section: Introductionmentioning

confidence: 99%

“…However, the VOC assessment of a very high number of samples, which is necessary to overcome the usually massive biological and genetic variability among fruit samples, may be laborious and time consuming. The so-called “phenotyping bottleneck”, caused by the absence of high-throughput and non-invasive methodologies, hampers an effective VOC assessment of broad plant collections ( Mazzucotelli et al, 2022 )). In fact, the use of conventional gas chromatographic (GC) techniques for VOC analysis presents many analytical limitations.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Volatilomics of raspberry fruit germplasm by combining chromatographic and direct-injection mass spectrometric techniques

Farneti

Khomenko

Ajelli

et al. 2023

Front. Mol. Biosci.

Self Cite

View full text Add to dashboard Cite

The application of direct-injection mass spectrometric (DI-MS) techniques, like Proton Transfer Reaction Time of Flight Mass Spectrometry (PTR-ToF-MS) has been suggested as a reliable phenotyping tool for fruit volatilome assessment in both genetic and quality-related studies. In this study the complexity of raspberry aroma was investigated by a comprehensive untargeted VOC analysis, done by combining SPME-GC-MS and PTR-ToF-MS assessments with multi-block discriminant analysis using the DIABLO mixOmics framework. The aim was to acquire an exhaustive characterization of the raspberry volatilome according to different fruit ripening stages (pink, ripe, and overripe) and genetic variances (50 accessions), as well as to investigate the potential of PTR-ToF-MS as a rapid and high throughput VOC phenotyping tool to address issues related to raspberry fruit quality. Results of this study demonstrated the complementarity between SPME-GC-MS and PTR-ToF-MS techniques to evaluate the raspberry aroma composition. PTR-ToF-MS generates reliable raspberry VOC fingerprints mainly due to a reduced compound fragmentation and precise content estimation. In addition, the high collinearity between isomers of monoterpenes and norisoprenoids, discovered by GC analysis, reduces the main analytic limitation of PTR-ToF-MS of not being able to separate isomeric molecules. The high similarity between the VOC matrices obtained by applying PTR-ToF-MS and SPME-GC-MS confirmed the possibility of using PTR-ToF-MS as a reliable high throughput phenotyping tool for raspberry volatiolome assessment. In addition, results provided by the germplasm collection investigation enabled to distinguish the best performing accessions, based on VOCs composition, to be used as superior parental lines for future breeding programs.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Volatilomics of raspberry fruit germplasm by combining chromatographic and direct-injection mass spectrometric techniques

Farneti

Khomenko

Ajelli

et al. 2023

Front. Mol. Biosci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Mazzucotelli et al additionally highlight the compliance of PTR-MS (and others) with the guidelines of green analytical chemistry. They highlight recent applications in food consumption and sensory, bioprocess monitoring, traceability, quality control, and high-throughput food volatilome phenotyping [ 124 ].…”

Section: Fast/screening Methods For On-site/mobile Analysis Of Vocsmentioning

confidence: 99%

On-Site Detection of Volatile Organic Compounds (VOCs)

Epping

Koch

2023

Molecules

View full text Add to dashboard Cite

Volatile organic compounds (VOCs) are of interest in many different fields. Among them are food and fragrance analysis, environmental and atmospheric research, industrial applications, security or medical and life science. In the past, the characterization of these compounds was mostly performed via sample collection and off-site analysis with gas chromatography coupled to mass spectrometry (GC-MS) as the gold standard. While powerful, this method also has several drawbacks such as being slow, expensive, and demanding on the user. For decades, intense research has been dedicated to find methods for fast VOC analysis on-site with time and spatial resolution. We present the working principles of the most important, utilized, and researched technologies for this purpose and highlight important publications from the last five years. In this overview, non-selective gas sensors, electronic noses, spectroscopic methods, miniaturized gas chromatography, ion mobility spectrometry and direct injection mass spectrometry are covered. The advantages and limitations of the different methods are compared. Finally, we give our outlook into the future progression of this field of research.

show abstract

“…DIMS instruments equipped with time-of-flight (PTR-TOF, VOCUS-CI-MS) or orbitrap mass analysers (SESI-MS) provide data with especially high informational content and reduce the need for the development of specific methods for different classes of target compounds. Based on these elements, and as recently highlighted by a review article [10], DIMS techniques show good compliance with green analytical chemistry principles [11], which advocate for analytical instruments and methods that require fewer harmful chemicals, minimise waste and guarantee improved operator safety.…”

Section: Discharge Reagent Ion Sourcementioning

confidence: 96%

Advances in the Application of Direct Injection Mass Spectrometry Techniques to the Analysis of Grape, Wine and Other Alcoholic Beverages

Romano,

Capozzi,

Khomenko

et al. 2023

Molecules

Self Cite

View full text Add to dashboard Cite

Direct injection mass spectrometry (DIMS) entails the direct introduction of a gaseous sample into a mass analyser without prior treatment or separation. DIMS techniques offer the opportunity to monitor processes in time, with limits of detection as low as 0.5 parts per trillion in volume (for a 1 s measurement time) while providing results with high informational content. This review provides insight into current and promising future developments of DIMS in the analysis of grape, wine and other alcoholic beverages. Thanks to its unique characteristics, DIMS allows the online monitoring of volatile organic compounds (VOCs) released by grapes during fermentative bioprocesses or by wine directly from the glass headspace or during drinking. A DIMS-based approach can also be adopted to perform quality control and high-throughput analysis, allowing us to characterise the volatile profile of large sample sets rapidly and in a comprehensive fashion. Furthermore, DIMS presents several characteristic elements of green analytical chemistry approaches, catalysing an interest linked to the development of sustainable paths in research and development activities in the field of viticulture and oenology.

show abstract

Proton transfer reaction mass spectrometry: A green alternative for food volatilome profiling

Cited by 10 publications

References 117 publications

Volatilomics of raspberry fruit germplasm by combining chromatographic and direct-injection mass spectrometric techniques

Volatilomics of raspberry fruit germplasm by combining chromatographic and direct-injection mass spectrometric techniques

On-Site Detection of Volatile Organic Compounds (VOCs)

Advances in the Application of Direct Injection Mass Spectrometry Techniques to the Analysis of Grape, Wine and Other Alcoholic Beverages

Contact Info

Product

Resources

About