Proton release by tea plant (Camellia sinensis L.) roots as affected by nutrient solution concentration and pH

Wan, Qianbing; Xu, Runsheng; Li, X. H.

doi:10.17221/326/2012-pse

Cited by 39 publications

(16 citation statements)

References 11 publications

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“…Second, the balance of H + , NH 4 + and NO 3 − could be used to explain this result. Tea roots took up a large amount of NH 4 + during growth and later released H + to maintain the charge balance in the plant body38. According to our current understanding of NO 3 − transportation, NO 3 − influx occurs with one H + symport, and two possible H + ions promote the inward transportation of one NO 3 − ion, while the efflux of H + is meant to balance the influx of NH 4 + .…”

Section: Discussionmentioning

confidence: 99%

Characteristics of NH4+ and NO3− fluxes in tea (Camellia sinensis) roots measured by scanning ion-selective electrode technique

Ruan

Wei

Wang

et al. 2016

Sci Rep

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As a vital beverage crop, tea has been extensively planted in tropical and subtropical regions. Nitrogen (N) levels and forms are closely related to tea quality. Based on different N levels and forms, we studied changes in NO3− and NH4+ fluxes in tea roots utilizing scanning ion-selective electrode technique. Our results showed that under both single and mixed N forms, influx rates of NO3− were much lower than those of NH4+, suggesting a preference for NH4+ in tea. With the increase in N concentration, the influx rate of NO3− increased more than that of NH4+. The NH4+ influx rates in a solution without NO3− were much higher than those in a solution with NO3−, while the NO3− influx rates in a solution without NH4+ were much lower than those in a solution with NH4+. We concluded that (1) tea roots showed a preference for NH4+, (2) presence of NO3− had a negative effect on NH4+ influx, and (3) NH4+ had a positive effect on NO3− influx. Our findings not only may help advance hydroponic tea experiments but also may be used to develop efficient fertilization protocols for soil-grown tea in the future.

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Section: Discussionmentioning

confidence: 99%

Characteristics of NH4+ and NO3− fluxes in tea (Camellia sinensis) roots measured by scanning ion-selective electrode technique

Ruan

Wei

Wang

et al. 2016

Sci Rep

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“…Yingshuang' (a cultivar of C. sinensis with good stress tolerance) were pre-incubated in control nutrient solution (Wan et al 2012) at 25 ± 1°C for 2 weeks in an artificial climate chamber under a 16-h light (240 lmol m -2 s -1 )/8-h dark cycle. Then, some of the pre-incubated plants were transferred to another artificial climate chamber and maintained at 4°C for low-temperature treatment.…”

Section: Plant Materials Growth Conditions and Abiotic Stress Treatmentioning

confidence: 99%

Overexpression of Camellia sinensis H1 histone gene confers abiotic stress tolerance in transgenic tobacco

Wang

et al. 2014

Plant Cell Rep

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Overexpression of CsHis in tobacco promoted chromatin condensation, but did not affect the phenotype. It also conferred tolerance to low-temperature, high-salinity, ABA, drought and oxidative stress in transgenic tobacco. H1 histone, as a major structural protein of higher-order chromatin, is associated with stress responses in plants. Here, we describe the functions of the Camellia sinensis H1 Histone gene (CsHis) to illustrate its roles in plant responses to stresses. Subcellular localization and prokaryotic expression assays showed that the CsHis protein is localized in the nucleus, and its molecular size is approximately 22.5 kD. The expression levels of CsHis in C. sinensis leaves under various conditions were investigated by qRT-PCR, and the results indicated that CsHis was strongly induced by various abiotic stresses such as low-temperature, high-salinity, ABA, drought and oxidative stress. Overexpression of CsHis in tobacco (Nicotiana tabacum) promoted chromatin condensation, while there were almost no changes in the growth and development of transgenic tobacco plants. Phylogenetic analysis showed that CsHis belongs to the H1C and H1D variants of H1 histones, which are stress-induced variants and not the key variants required for growth and development. Stress tolerance analysis indicated that the transgenic tobacco plants exhibited higher tolerance than the WT plants upon exposure to various abiotic stresses; the transgenic plants displayed reduced wilting and senescence and exhibited greater net photosynthetic rate (Pn), stomatal conductance (Gs) and maximal photochemical efficiency (Fv/Fm) values. All the above results suggest that CsHis is a stress-induced gene and that its overexpression improves the tolerance to various abiotic stresses in the transgenic tobacco plants, possibly through the maintenance of photosynthetic efficiency.

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“…The tea plants were grown under a 12-h light (28 °C)/12-h dark (22 °C) photoperiod regime during the first cultivation stage. Following this, the C. sinensis plants were grown for 1 month in a nutrient solution described by Wan et al 44,. and then treated with 0.5 (control) or 250 μM MnSO 4 (excess exposure) for 14 d. The leaves and roots were separately harvested, washed with deionized water, weighed, and stored at −80 °C for further analysis.…”

Section: Methodsmentioning

confidence: 99%

Metal transport protein 8 in Camellia sinensis confers superior manganese tolerance when expressed in yeast and Arabidopsis thaliana

et al. 2017

Sci Rep

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Manganese (Mn) is an important micronutrient element required for plant growth and development, playing catalytic roles in enzymes, membranes and DNA replication. The tea plant (Camellia sinensis) is able to accumulate high concentration of Mn without showing signs of toxicity, but the molecular mechanisms underlying this remain largely unknown. In this study, the C. sinensis cultivar ‘LJCY’ had higher Mn tolerance than cultivar ‘YS’, because chlorophyll content reduction was lower under the high Mn treatment. Proteomic analysis of the leaves revealed that C. sinensis Metal Tolerance Protein 8 (CsMTP8) accumulated in response to Mn toxicity in cultivar ‘LJCY’. The gene encoding CsMTP8, designated as CsMTP8 was also isolated, and its expression enhanced Mn tolerance in Saccharomyces cerevisiae. Similarly, the overexpression of CsMTP8 in Arabidopsis thaliana increased plant tolerance and reduced Mn accumulation in plant tissues under excess Mn conditions. Subcellular localization analysis of green florescence fused protein indicated that CsMTP8 was localized to the plasma membranes. Taken together, the results suggest that CsMTP8 is a Mn-specific transporter, which is localized in the plasma membrane, and transports excess Mn out of plant cells. The results also suggest that it is needed for Mn tolerance in shoots.

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Proton release by tea plant (Camellia sinensis L.) roots as affected by nutrient solution concentration and pH

Cited by 39 publications

References 11 publications

Characteristics of NH4+ and NO3− fluxes in tea (Camellia sinensis) roots measured by scanning ion-selective electrode technique

Characteristics of NH4+ and NO3− fluxes in tea (Camellia sinensis) roots measured by scanning ion-selective electrode technique

Overexpression of Camellia sinensis H1 histone gene confers abiotic stress tolerance in transgenic tobacco

Metal transport protein 8 in Camellia sinensis confers superior manganese tolerance when expressed in yeast and Arabidopsis thaliana

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