Proton NMR studies of muscle metabolites in vivo

Williams, S. R.; Gadian, David G.; Proctor, E; Sprague, David; Talbot, Delphine; Young, I. R.; Brown, Francis F.

doi:10.1016/0022-2364(85)90336-1

Cited by 48 publications

(29 citation statements)

References 16 publications

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“…[23][24][25]), few have examined the quantification problem. Because of the need to suppress the intense Hz0 signal and to simplify the spectra, spinecho pulse sequences are used.…”

Section: Discussionmentioning

confidence: 99%

NMR‐invisible lactate in blood plasma

et al. 1988

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Resonances for lactate are broadened in 500 MHz 1H NMR spectra of human blood plasma and only about one‐third is visible in Hahn spin‐echo spectra. Similar effects are observed for some other carboxylate anions. Lactate added to the high‐M r fraction of plasma can give rise to peaks which are too broad to observe in either single‐pulse or spin‐echo spectra. Addition of agents such as NH4Cl of SDS dramatically increases the intensities of lactate peaks. Some glycoproteins appear to broaden lactate resonances.

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“…[23][24][25]), few have examined the quantification problem. Because of the need to suppress the intense Hz0 signal and to simplify the spectra, spinecho pulse sequences are used.…”

Section: Discussionmentioning

confidence: 99%

NMR‐invisible lactate in blood plasma

et al. 1988

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“…In certain cases, the spin-echo alone is sufficient to suppress the water resonance, as its T2 is much shorter than that from metabolites (Williams et al 1985). In addition to suppressing water, broad signals from proteins are removed from the spectrum if a spin-echo sequence is used, which can simplify the interpretation of spectra.…”

Section: Technical Considerationsmentioning

confidence: 99%

“…These resonances are pH sensitive in the physiological region, and can be used to determine intracellular pH (Williams et al 1985). A similar compound, carnosine, is present in frog muscle and has been used both to measure pH (Yoshizaki et al 1981;Arus et al 1984) and to correlate pH changes with lactate production (Seo, Yoshizaki & Morimoto, 1983), also measured by 'H n.m.r.…”

Section: Applicationsmentioning

confidence: 99%

“…have now been described (Rothman, Behar, Hetherington & Shulman, 1984b;Williams et al 1985) which enable certain spin-coupled signals to be picked out from the spectrum. These techniques are based on the spin-echo double resonance experiment described by Campbell & Dobson (1979), and they have been applied in vivo to detect lactate production in brain (Rothman et al 1984b) and muscle (Williams et al 1985) despite the presence of the very large signal from triglycerides. Various other compounds have also been detected in vivo (Rothman et al 1984b) and in excised tissues (Rothman, Arias-Mendoza, Shulman & Shulman, 1984a) using such methods.…”

Section: Technical Considerationsmentioning

confidence: 99%

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TISSUE METABOLISM STUDIED IN VIVO BY NUCLEAR MAGNETIC RESONANCE

Williams

Gadian

1986

Exp Physiol

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N.m.r. is finding increasing application as a non‐invasive method of studying metabolism in vivo. 31P n.m.r. is particularly useful for studies of energy metabolism, while other nuclei, in particular 1H, 13C and 19F, can provide additional information about certain aspects of cellular metabolism that are not amenable to investigation by 31P n.m.r. The major problem with n.m.r. is lack of sensitivity. The main implications of this are first that signals can only be detected from metabolites that are present at fairly high concentrations, and secondly that the spatial resolution for localized studies, particularly of internal regions of the body, is limited. N.m.r. could considerably enhance our understanding of metabolic abnormalities in disease, but the precise role of such metabolic studies in a clinical setting remains to be evaluated.

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“…The MAS spectra in Figure 7.3 b) shows several more resonances. The assignments known from previously published work [54,61] are presented in the figure caption. Figure 7.3 c) shows the appropriately projected p-MAS spectrum after a shearing transformation and re-scaling to the isotropic spectrum, using λ .…”

Section: Resultsmentioning

confidence: 99%

New Methodology For Use in Rotating Field Nuclear MagneticResonance

Jachmann

2007

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High-resolution NMR spectra of samples with anisotropic broadening are simplified to their isotropic spectra by fast rotation of the sample at the magic angle 54.7 • . This dissertation concerns the development of novel Nuclear Magnetic Resonance(NMR) methodologies which would rotate the magnetic field instead of the sample, i.e. rotating field NMR. It also provides an overview of the NMR concepts, procedures, and experiments needed to understand the methodologies that will be used for rotating field NMR.A simple two-dimensional shimming method based on harmonic corrector rings provides arbitrary multiple order shimming corrections that are necessary for rotating field systems, but can be used in shimming other systems as well. Those results demonstrate, for example, that quadrupolar order shimming improves the linewidth by up to a factor of ten. An additional order of magnitude reduction is in principle achievable by utilizing this shimming method for z-gradient correction and higher order xy gradients.Additionally, initial investigations into a specialized pulse sequence for the rotating field NMR experiment, which allows for spinning at angles other than the magic angle and spinning slower than the anisotropic broadening is discussed. This will be useful for rotating field NMR because there are limits on how fast a field can be spun and difficulties of reaching the magic angle. This pulse sequence is a combination of the previously established projected magic angle spinning (p-MAS) and magic angle turning (MAT) pulse sequences. One of the goals of this project is for rotating field NMR to be used on biological systems. The p-MAS pulse sequence was successfully tested on bovine tissue samples, which suggests that it will be a viable methodology to use in rotating field NMR.A side experiment on steering magnetic particles by MRI gradients was also carried out.Initial investigations indicate some movement, but for total steering control, further experiments are needed.1

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Proton NMR studies of muscle metabolites in vivo

Cited by 48 publications

References 16 publications

NMR‐invisible lactate in blood plasma

NMR‐invisible lactate in blood plasma

TISSUE METABOLISM STUDIED IN VIVO BY NUCLEAR MAGNETIC RESONANCE

New Methodology For Use in Rotating Field Nuclear MagneticResonance

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