Proton microbeams, their production and use

Cookson, J.A.; Ferguson, A.T.G.; Pilling, F.D.

doi:10.1007/bf02520973

Cited by 206 publications

(11 citation statements)

References 8 publications

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“…The first micro-PIXE system was developed at Harwell (Cookson et al 1972) shortly after the introduction of the PIXE method by Johansson et al (1970). PIXE is based on the detection of characteristic X-rays emitted by the elements of a sample when excited with an MeV ion beam.…”

Section: Particle-induced X-ray Emissionmentioning

confidence: 99%

Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Ortega

Devès

Carmona

2009

J. R. Soc. Interface.

139

131

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The direct detection of biologically relevant metals in single cells and of their speciation is a challenging task that requires sophisticated analytical developments. The aim of this article is to present the recent achievements in the field of cellular chemical element imaging, and direct speciation analysis, using proton and synchrotron radiation X-ray micro-and nanoanalysis. The recent improvements in focusing optics for MeV-accelerated particles and keV X-rays allow application to chemical element analysis in subcellular compartments. The imaging and quantification of trace elements in single cells can be obtained using particle-induced X-ray emission (PIXE). The combination of PIXE with backscattering spectrometry and scanning transmission ion microscopy provides a high accuracy in elemental quantification of cellular organelles. On the other hand, synchrotron radiation X-ray fluorescence provides chemical element imaging with less than 100 nm spatial resolution. Moreover, synchrotron radiation offers the unique capability of spatially resolved chemical speciation using micro-X-ray absorption spectroscopy. The potential of these methods in biomedical investigations will be illustrated with examples of application in the fields of cellular toxicology, and pharmacology, bio-metals and metal-based nano-particles.

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Section: Particle-induced X-ray Emissionmentioning

confidence: 99%

Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Ortega

Devès

Carmona

2009

J. R. Soc. Interface.

139

131

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“…Cookson et al developed the first proton micro-beam system in 1970 and obtained a beam spot size of 4 um [6]. A micro-beam size of < ljim was achieved by the Oxford group using triplet quadrupole magents [7].…”

Section: Micro-beam Systemmentioning

confidence: 99%

Micron-Ct Using Pixe With Micro-Beams

et al. 2005

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We developed a micron-CT consisting of micro-beam system and X-ray CCD camera (Hamamatsu photonics C880OX), whose element size is 8umX8um and a total number of image elements 1000 X 1000 gives an image size of 8mm X 8mm. The sample is placed in a tube of a small diameter, which is rotated by a stepping motor. The transmission data through the sample are taken with characteristic Ti-K-X-rays (4.558 keV) produced by 3MeV proton and a particle micro-beams. After image reconstruction using an iteration method, 3D-images of small objects namely, hair and small ants were obtained with a spatial resolution of -5um. It is expected that our micron-CT can provide cross sectional images of in-vivo cellular samples with high resolution and can be applied to a wide range of research in biology and medicine.

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“…The first setup of a "nuclear microprobe" with a beam spot of ~ 10 jum was done at Harwell (Cookson, 1972). Since then, about a dozen facilities have been built.…”

Section: A Micro Ion-beam Productionmentioning

confidence: 99%