Proton magnetic resonance analysis of terbium ion-nucleic acid complexes: Further evidence for two-site binding to polynucleotides

Gross, David S.; Simpkins, Henry; Bubienko, Edward; Borer, Philip N.

doi:10.1016/0003-9861(82)90172-2

Cited by 9 publications

(3 citation statements)

References 36 publications

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“…This is not seen in the luminescence studies, which suggests that at the level of the first coordination sphere of the lanthanide ion there is little difference between coordination by inorganic phosphate and phosphate groups on the DNA. However, at pH 9, no signals could be observed in the 31 P NMR spectra of ss-DNA in the presence of the same concentration of Tb(III), due to severe broadening, suggesting that the interaction between metal ions and ss-DNA is stronger, probably, as previously suggested [83], involving both phosphate groups and the N-donor groups of the bases.…”

Section: P Nuclear Magnetic Resonance Studiesmentioning

confidence: 63%

“…These results also suggest that at these concentrations a maximum of one GTP molecule is bound to each lanthanide ion. Increases in the intensity of the emission bands, particularly in the presence of DNA and RNA, has been attributed to energy transfer [66]. However, as we have previously discussed [32] we believe that this is not the only factor involved, and that changes in hydration of the cation may also be responsible.…”

Section: Luminescence Studiesmentioning

confidence: 94%

“…Such two-site binding has previously been suggested from both luminescence [65] and 1 H NMR spectroscopy [66] Phosphorous-31 NMR spectroscopy has proved to be a powerful technique here for studying the conformation, dynamics and binding of nucleic acids in aqueous solutions [67][68][69]. We have therefore used this technique for characterizing the interactions between nucleic acids and lanthanides in these systems.…”

Section: Introductionmentioning

confidence: 96%

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Using lanthanides as probes for polyelectrolyte–metal ion interactions. Hydration changes on binding of trivalent cations to nucleotides and nucleic acids

et al. 2008

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a b s t r a c tThe interaction of the trivalent lanthanide ions Ce(III) and Tb(III) with ribonucleic acid (RNA) and guanosine 5 0 -triphosphate (GTP) in aqueous solution has been studied using their luminescence spectra and decays. Complexation is indicated by changes in luminescence intensity. With the system terbium(III)-RNA and terbium(III)-GTP, changes in luminescence with pH are probably related to conformational changes on the structure and also to the different degrees of protonation of phosphate groups and nucleotide bases. The degree of hydration of Tb(III) on binding to RNA and GTP is followed by luminescence lifetime measurements in water and deuterium oxide solutions, and at least one hydration water is lost from the lanthanide ion on binding to RNA or GTP at pH 4.7 and pH 7. Rather different behavior is observed on binding RNA at pH 9, where six water molecules are lost, possibly due to the lanthanide binding to the bases of the RNA backbone. This is similar to what has previously been seen with DNA, and is supported by 31 P NMR spectral measurements, which confirm the possibility of lanthanide binding to both phosphate and bases in the nucleic acids. In the case of GTP at pH 9, two water molecules appear to be lost, probably due to terbium binding both to guanine and a phosphate group. Gd(III) EPR spectral measurements with DNA, RNA and GTP suggest decreased lanthanide ion mobility on binding.

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Section: P Nuclear Magnetic Resonance Studiesmentioning

confidence: 63%

Section: Luminescence Studiesmentioning

confidence: 94%