Proton induced X-ray emission (PIXE) technique for determining multi-element composition of transformed hairy root cultures of Boerhaavia diffusa L.: an important medicinal herb

Advances in Boerhaavia diffusa hairy root technology: a valuable pursuit for identifying strain sensitivity and up-scaling factors to refine metabolite yield and bioactivity potentials

“…The presently observed exclusive susceptibility of leaf explants and total failure of stem segments towards A. rhizogenes-mediated hairy root induction contradict the earlier findings of Sahu et al (2013Sahu et al ( , 2014, who have demonstrated notable susceptibility of intermodal segments of in vivo-grown B. diffusa plants towards the peak limit of hairy root induction. The disparity in the maturity levels of the utilized explants could be one of the causes for the observed difference in susceptibility of the nodal segments, as the maturation status of explants predominantly impacts the availability of functionally competent cells (as well as their internal hormonal levels), which essentially serve as ideal targets for hairy root induction (Potrykus 1990).…”

“…Moreover, since roots are the chief source of the therapeutically active constituents of B. diffusa (Sahu et al 2014), their exclusive authentic collection implies utmost significance to comply with the characteristic pharmaceutical traits of the raw material. However, the uninhibited adulteration of its roots with that of Trianthema portulacastrum imparts serious practical challenge, which cannot only inadvertently jeopardize the overall bioactivity quotient of its metabolites but also can inflict detrimental effects on the curative benefits of its roots (Gomes et al 2013).…”

Elemental Composition of Infusions of Herbs (Tisanes) of North Ossetia (the Caucasus)

“…It has been shown that botanical species of medicinal plants cultivated in different climatic conditions, on various types of soil, have a significantly different chemical composition and, subsequently, different content of secondary metabolites, such as flavonoids, alkaloids, terpenoids, quinones, steroids etc. [35,36]. Common preparation methods include decoctions, macerates, herbal teas, and herbal tea mixtures [37,38].…”

PIXE‐based detection of elemental accumulation during direct organogenesis in Blepharispermum subsessileDC.: An endangered medicinal plant of Odisha, India

“…[20][21][22] A few reports were available on the use of X-ray emission techniques such as ED-XRF and PIXE for obtaining trace element profiles of in vitro culture systems. [15,[23][24][25][26][27][28] Among the available reports, Desai et al [24] studied the elemental variation during different developmental stages of somatic embryogenesis from dedifferentiated callus to somatic embryo-derived plants using ED-XRF. In the present study, we tried to ascertain the variation in elemental accumulation during different developmental stages of direct organogenesis from cotyledons of B. subsessile.…”

“…It has been successfully used for quantitative elemental characterization of a wide range of complex materials such as soil, honey, environmental samples, environmental and metallurgical samples, and in natura plant samples . A few reports were available on the use of X‐ray emission techniques such as ED‐XRF and PIXE for obtaining trace element profiles of in vitro culture systems . Among the available reports, Desai et al studied the elemental variation during different developmental stages of somatic embryogenesis from dedifferentiated callus to somatic embryo‐derived plants using ED‐XRF.…”