Proton extrusion by leaf discs ofVicia faba L.: light- and ion-stimulated H(su+) release†

Petzold, U.; Dahse, I.

doi:10.1007/bf02878482

Cited by 15 publications

(11 citation statements)

References 28 publications

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“…The H + extrusion observed by Shimazaki et al [107] was in accordance with electrophysiological studies demonstrating membrane hyperpolarization in response to blue light [97]. Acidification of the apoplastic pH in response to light was observed in other experiments as well [111,112], although some reports also demonstrate a biphasic response to light, where medium acidification is preceded by an alkalinization, most likely involving photosynthetic processes [113,114].…”

Section: Blue Light and Membrane Hyperpolarizationsupporting

confidence: 68%

Ion channels in plant signaling

Zimmermann

Ehrhardt

Plesch

et al. 1999

CMLS, Cell. Mol. Life Sci.

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Plant ion channel activities are rapidly modulated in response to several environmental and endogenous stimuli such as light, pathogen attack and phytohormones. Electrophysiological as well as pharmacological studies provide strong evidence that ion channels are essential for the induction of specific cellular responses, implicating their tight linkage to signal transduction cascades. Ion channels propagate signals by modulating the membrane potential or by directly affecting cellular ion composition. In addition, they may also be effectors at the end of signaling cascades, as examplified by ion channels which determine the solute content of stomatal guard cells. Plant channels are themselves subject to regulation by a variety of cellular factors, including calcium, pH and cyclic nucleotides. In addition, they appear to be regulated by (de)-phosphorylation events as well as by direct interactions with cytoskeletal and other cellular proteins. This review summarizes current knowledge on the role of ion channels in plant signaling.

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Section: Blue Light and Membrane Hyperpolarizationsupporting

confidence: 68%

Ion channels in plant signaling

Zimmermann

Ehrhardt

Plesch

et al. 1999

CMLS, Cell. Mol. Life Sci.

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“…Moreover, photosynthesis is required to activate the PM H + -ATPase in green leaf cells of Elodea (Jeschke 1970;Tazawa et al 1986;MarreÁ et al 1989) and several algae (see Introduction). Since DCMU causes at least a partial inhibition of light-stimulated growth and H + net eux in leaves of dicot land plants as well (Van Volkenburgh and Cleland 1980;Petzold and Dahse 1988;Blum et al 1992), it could be that land plants also share this mechanism. However, the results of this study reject such a generalization.…”

Section: Discussionmentioning

confidence: 96%

“…Although DCMU inhibits extracellular acidi®cation by illuminated discs or protoplast from dicot leaves (e.g. Kelly 1983;Petzold and Dahse 1988;, its eect on leaf growth never reaches the level of complete inhibition Blum et al 1992). Even if chlorophyll is the primary photoreceptor, some lightstimulated leaf growth might still have occurred because DCMU was only partially eective in inhibiting photosynthesis.…”

Section: Introductionmentioning

confidence: 98%

Chlorophyll is not the primary photoreceptor for the stimulation of P-type H + pump and growth in variegated leaves of Coleus × hybridus

Stahlberg

Volkenburgh

Cleland

2000

Planta

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There has been persisting controversy over the role of photosynthesis in the stimulation of the plasma membrane H+-ATPase and growth of dicotyledonous leaves by light. To investigate this, we compared the effects of light on growth, H+ net efflux and membrane potential (Vm) of strips which contained either only chlorophyll-free (white) mesophyll cells or chlorophyll-containing (green) cells cut from variegated Coleus leaves. White mesophyll cells responded to white, blue and red light with a hyperpolarization of Vm, an acidification of the apoplast and a promotion of growth, all of which began after a lag of 2-7 min. In contrast, green mesophyll cells showed a biphasic light response in which the hyperpolarization and the acidification were preceded by a rapid depolarization of Vm and an alkalinization of the apoplast. Nevertheless, green and white tissues showed comparable growth promotions in response to light. The light response of the leaf mesophyll is a composite of two separate photosystems. The initial depolarization and alkalinization are mediated by photosynthesis and blocked by 3-(3,4-dichlorophenyl)-1,1-dimethylurea. The slower hyperpolarization, acidification and growth response, on the other hand, are clearly in response to light absorption by pigments other than chlorophyll.

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“…Light promotes leaf expansion and also stimulates proton extrusion by mesophyll cells in V. faba and other species (25,26). Proton extrusion is associated with membrane hyperpolarization, which may drive solute uptake (27 …”

Section: Discussionmentioning

confidence: 99%

Characterization of a G-protein-regulated outward K+ current in mesophyll cells of vicia faba L.

Assmann²

1993

Proc. Natl. Acad. Sci. U.S.A.

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Whole-cell voltage-dependent currents in isolated mesophyll protoplasts of Viciafaba were investigated by patch-clamp techniques. With 104 mM K+ in the cytosol and 13 mM K+ in the external solution, depolarization of the plasma membrane from -47 mV to potentials between -15 and +85 mV activated a voltage-and time-dependent outward current (4.Ju). The average magnitude of lot at +85 mV was 28.5 ± 3.3 pA-pF-1. No inward voltage-dependent current was observed upon hyperpolarization of the plasma membrane from -55 mV to potentials as negative as -175 mV. Timeactivated outward current was blocked by Ba2+ (1 mM BaCl2) and was not observed when K+ was eliminated from the external and internal solutions, indicating that this outward current was carried primarily by K+ ions. The voltage dependency of outward K+ current revealed a possible mechanism for K+ efflux from mesophyll cells. A GDP analogue guanosine 5'-[fl-thioJdiphosphate (500 jpM) significantly enhanced outward K+ current. The outward K+ current was inhibited by the GTP analogue guanosine 5'- [(-thiojtriphosphate (500 ,M) and by an increase in cytoplasmic free Ca2' concentrations. Cholera toxin, which ADP-ribosylates guanine nucleotide-binding regulatory proteins, also inhibited outward K+ current. These findings illustrate the presence in mesophyll cells of outwardrectifying K+ channels that are regulated by GTP-binding proteins and calcium.

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Proton extrusion by leaf discs ofVicia faba L.: light- and ion-stimulated H(su+) release†

Cited by 15 publications

References 28 publications

Ion channels in plant signaling

Ion channels in plant signaling

Chlorophyll is not the primary photoreceptor for the stimulation of P-type H + pump and growth in variegated leaves of Coleus × hybridus

Characterization of a G-protein-regulated outward K+ current in mesophyll cells of vicia faba L.

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