Proton dependence of tobacco mosaic virus dissociation by pressure

Santos, Jose L.R.; Bispo, José Ailton Conceição; Landini, Gustavo Fraga; Bonafé, Carlos Francisco Sampaio

doi:10.1016/j.bpc.2004.04.003

Cited by 27 publications

(23 citation statements)

References 53 publications

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“…This finding will be helpful to determine an optimal pressure to treat food products with different pH values. Our observation is consistent with the previous reports for FCV (16) and tobacco mosaic virus (8,51), which were also more resistant to HPP at lower pH. In contrast, a significantly enhanced inactivation of HAV was found in acidic environments (35)(36)(37).…”

Section: Discussionsupporting

confidence: 93%

Inactivation of a Human Norovirus Surrogate by High-Pressure Processing: Effectiveness, Mechanism, and Potential Application in the Fresh Produce Industry

Lou¹,

Neetoo

Chen

et al. 2011

Appl Environ Microbiol

113

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Fresh produce is often a high-risk food for norovirus contamination because it can become contaminated at both preharvest and postharvest stages and it undergoes minimal or no processing. Currently, there is no effective method to eliminate the viruses from fresh produce. This study systematically investigated the effectiveness of high-pressure processing (HPP) on inactivating murine norovirus (MNV-1), a surrogate for human norovirus, in aqueous medium and fresh produce. We demonstrated that MNV-1 was effectively inactivated by HPP. More than a 5-log-PFU/g reduction was achieved in all tested fresh produce when it was pressurized at 400 MPa for 2 min at 4°C. We found that pressure, pH, temperature, and food matrix affected the virus survival in foods. MNV-1 was more effectively inactivated at 4°C than at 20°C in both medium and fresh produce. MNV-1 was also more sensitive to HPP at neutral pH than at acidic pH. We further demonstrated that disruption of viral capsid structure, but not degradation of viral genomic RNA, is the primary mechanism of virus inactivation by HPP. However, HPP does not degrade viral capsid protein, and the pressurized capsid protein was still antigenic. Overall, HPP had a variable effect on the sensorial quality of fresh produce, depending on the pressure level and type of product. Taken together, HPP effectively inactivated a human norovirus surrogate in fresh produce with a minimal impact on food quality and thus can provide a novel intervention for processing fruits intended for frozen storage and related products such as purees, sauces, and juices.

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Section: Discussionsupporting

confidence: 93%

Inactivation of a Human Norovirus Surrogate by High-Pressure Processing: Effectiveness, Mechanism, and Potential Application in the Fresh Produce Industry

Lou¹,

Neetoo

Chen

et al. 2011

Appl Environ Microbiol

113

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“…Pressure-induced denaturation has been widely applied in virology. Whereas the primary structure of proteins is unchanged at pressures as high as 10 kbar, dissociation is nevertheless quite frequently observed (31,32,50,51). In general, the effect of pressure on a physicochemical process at equilibrium is governed by the volume change of the process, DV (31,32).…”

Section: Discussionmentioning

confidence: 99%

“…At higher pressures, nonspecific aggregates of capsid proteins predominate over seemingly complete reassociated capsids. The formation of imperfect virus particles after a cycle of compression and decompression has also been demonstrated for human norovirus (49) and rotavirus (41,46) by electron microscopy, gel filtration, and spectroscopy (8,50,51,53). In this article, we describe the effects of pressure and chemical treatments on TMV by mapping the linear epitopes through spot synthesis and comparing them to results obtained with epitope prediction software.…”

mentioning

confidence: 99%

“…tudies in recent years have demonstrated the reversible dissociation of oligomeric proteins by hydrostatic pressure (9,51). Observations of comparable effects on multi-subunit proteins and viruses have led to the idea of using hydrostatic pressure to suppress virus infectivity, while preserving or perhaps improving the immunogenic properties.…”

mentioning

confidence: 99%

“…Observations of comparable effects on multi-subunit proteins and viruses have led to the idea of using hydrostatic pressure to suppress virus infectivity, while preserving or perhaps improving the immunogenic properties. In most cases, effective immunization against viruses requires presentation of the whole virus particle to the immune system (9,50,51). This requirement, coupled to the need to eliminate infectivity, greatly limits the possibilities of preparing appropriate vaccines.…”

mentioning

confidence: 99%

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Influence of High Hydrostatic Pressure on Epitope Mapping of Tobacco Mosaic Virus Coat Protein

Neto

Sampaio²,

Arns³

2014

Viral Immunology

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In this study, we investigated the effect of high hydrostatic pressure (HHP) on tobacco mosaic virus (TMV), a model virus in immunology and one of the most studied viruses to date. Exposure to HHP significantly altered the recognition epitopes when compared to sera from mice immunized with native virus. These alterations were studied further by combining HHP with urea or low temperature and then inoculating the altered virions into Balb-C mice. The antibody titers and cross-reactivity of the resulting sera were determined by ELISA. The antigenicity of the viral particles was maintained, as assessed by using polyclonal antibodies against native virus. The antigenicity of canonical epitopes was maintained, although binding intensities varied among the treatments. The patterns of recognition determined by epitope mapping were cross checked with the prediction algorithms for the TMVcp amino acid sequence to infer which alterations had occurred. These findings suggest that different cleavage sites were exposed after the treatments and this was confirmed by epitope mapping using sera from mice immunized with virus previously exposed to HHP.

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Bioinspired Artificial Tobacco Mosaic Virus with Combined Oncolytic Properties to Completely Destroy Multidrug‐Resistant Cancer

Zhong

Zhang

et al. 2020

Advanced Materials

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Although biomimetic virus‐like strategies have been widely used in antitumor applications, construction of uniquely shaped virus‐like agents and optimization of their specific morphological features to achieve diverse antitumor functions are worthwhile pursuits. Here, a novel strategy to construct an artificial tobacco mosaic virus (ATMV) that closely mimics the structure of the rod‐like tobacco mosaic virus (TMV) is developed. The supramolecular array is self‐assembled from small, repeated subunits of tailor‐made capsid‐mimicking dendrons onto RGD‐modified single‐walled carbon nanotube to construct the ATMVs with high structural stability. The ATMVs are tactfully designed with shielding, targeting, and arming approaches, including shielding the viruses against premature elimination, selectively targeting tumor tissue, and arming the viruses with oncolytic abilities. The elongated particles are concealed in blood until they arrived at a tumor site, then they induce robust composite oncolytic processes including cytomembrane penetration, endoplasmic reticulum disruption to cause Ca2+ release, chemotherapeutic delivery, and photothermal therapy. Excitingly, the ATMVs not only lyse primary infected cells, but permeate adjacent cells for secondary infection, spreading cell‐to‐cell and continuing to induce lysis even deep in solid tumors. This work inspires a uniquely shaped virus‐like agent with tactically optimized oncolytic functions that completely defeated large drug‐resistant colon tumor (LoVo/Adr, ≈500 mm3).

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Proton dependence of tobacco mosaic virus dissociation by pressure

Cited by 27 publications

References 53 publications

Inactivation of a Human Norovirus Surrogate by High-Pressure Processing: Effectiveness, Mechanism, and Potential Application in the Fresh Produce Industry

Inactivation of a Human Norovirus Surrogate by High-Pressure Processing: Effectiveness, Mechanism, and Potential Application in the Fresh Produce Industry

Influence of High Hydrostatic Pressure on Epitope Mapping of Tobacco Mosaic Virus Coat Protein

Bioinspired Artificial Tobacco Mosaic Virus with Combined Oncolytic Properties to Completely Destroy Multidrug‐Resistant Cancer

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