Protoluciola albertalleni gen.n., sp.n., a new Luciolinae firefly (Insecta: Coleoptera: Lampyridae) from Burmite amber

Казанцев, С. В.

doi:10.15298/rusentj.24.4.02

Cited by 16 publications

(23 citation statements)

References 3 publications

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“…2 e). The phylogenetic analysis of mitogenomic gene and rDNA genes 19 , 22 and our comparison on morphology of Lamprigera with that of a recently described fossil species in Luciolinae 23 support the close relationship between Lamprigera and Luciolinae. These combined data demonstrate that L. yunnana has a closer phylogenetic relationship to Luciolinae than Lampyrinae, and thus L. yunnana should be a member of Luciolinae rather than a species in Lampyrinae.…”

Section: Resultssupporting

confidence: 78%

Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies

Zhang

Dong

et al. 2020

Sci Rep

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Fireflies are among the most charismatic insects for their spectacular bioluminescence, but the origin and evolution of bioluminescence remain elusive. Especially, the genic basis of luciferin (d-luciferin) biosynthesis and light patterns is largely unknown. Here, we present the high-quality reference genomes of two fireflies Lamprigera yunnana (1053 Mb) and Abscondita terminalis (501 Mb) with great differences in both morphology and luminous behavior. We sequenced the transcriptomes and proteomes of luminous organs of two species. We created the CRISPR/Cas9-induced mutants of Abdominal B gene without luminous organs in the larvae of A. terminalis and sequenced the transcriptomes of mutants and wild-types. Combining gene expression analyses with comparative genomics, we propose a more complete luciferin synthesis pathway, and confirm the convergent evolution of bioluminescence in insects. Using experiments, the function of the firefly acyl-CoA thioesterase (ACOT1) to convert l-luciferin to d-luciferin was validated for the first time. Comparisons of three-dimension reconstruction of luminous organs and their differentially expressed genes among two species suggest that two positive genes in the calcium signaling pathway and structural difference of luminous organs may play an important role in the evolution of flash pattern. Altogether, our results provide important resources for further exploring bioluminescence in insects.

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Section: Resultssupporting

confidence: 78%

Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies

Zhang

Dong

et al. 2020

Sci Rep

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“…Lamprigera luciferase shows higher amino acid identity to those of Cyphonocerinae and Lampyrinae than to those of Luciolinae (Table ), which agrees with the similar luminous traits of this taxon to Lampyrinae and Cyphonocerinae (continuously yellow‐green glow). On the other hand, a recently described fossil species in Luciolinae, which showed general morphology of both Luciolinae and Lamprigera , supported the close relationship of Lamprigera to Luciolinae as shown in ML/BI trees (Fig. B).…”

Section: Resultssupporting

confidence: 69%

Cloning and Characterization of Luciferase from the Chinese Firefly Lamprigera yunnana

Liu

Zhang

Hou

et al. 2019

Photochem & Photobiology

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Lamprigera (Lampyridae) is a small genus with only 17 species distributing in Asian countries. Its larviform females and alate males can produce continuously strong yellow‐green light at night. However, no luciferase gene was reported for this genus and its subfamily‐level phylogenetic position still remains uncertain. Here, we cloned the luciferase gene from one Chinese species, Lamprigera yunnana, by reverse transcription‐polymerase chain reaction (RT‐PCR) and rapid amplification of cDNA ends (RACE). This luciferase includes 551 deduced amino acids (AA) with the sequence identity of 71.8–76.8%, 67.5–70.7%, 68.4–75.3%, 77.8% and 59.5% to those from Lampyrinae, Luciolinae, Ototretinae, Cyphonocerinae and Photurinae, respectively. Phylogenetic analyses of deduced AA of luciferases suggest that Lamprigera locates outside Lampyrinae, in which it was originally placed in traditional taxonomy. The luciferase was produced in vitro as recombinant protein, and its biochemical properties were characterized. It possesses significant luminescence activity at pH 7.8, and its KM for D‐luciferin and ATP are 61 μm and 122 μm, respectively. It shows the highest activity at 37°C and is completely inactivated at 55°C. It is pH‐sensitive with the maximum emission spectrum of 566 nm at pH 7.8. Our data provide new insights into Lamprigera luciferase and its phylogenetic position.

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“…4). This dating was also consistent approximately to the oldest fossil record of ancient firefly having a photophore structure on the abdominal segment, which dated back to around 100 Ma ago (6).…”

Section: Molecular Clock Analysessupporting

confidence: 80%

“…S6) are indicted on each node. The geohistorical positions of the oldest firefly fossil with abdominal photophore structure(6) and gene duplication between Luc1-type and Luc2-type genes are indicated on the phylogeny or geological time scale.on December 13, 2020 http://advances.sciencemag.org/ Downloaded from…”

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confidence: 99%

Resurrecting the ancient glow of the fireflies

et al. 2020

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The color of firefly bioluminescence is determined by the structure of luciferase. Firefly luciferase genes have been isolated from more than 30 species, producing light ranging in color from green to orange-yellow. Here, we reconstructed seven ancestral firefly luciferase genes, characterized the enzymatic properties of the recombinant proteins, and determined the crystal structures of the gene from ancestral Lampyridae. Results showed that the synthetic luciferase for the last common firefly ancestor exhibited green light caused by a spatial constraint on the luciferin molecule in enzyme, while fatty acyl-CoA synthetic activity, an original function of firefly luciferase, was diminished in exchange. All known firefly species are bioluminescent in the larvae, with a common ancestor arising approximately 100 million years ago. Combined, our findings propose that, within the mid-Cretaceous forest, the common ancestor of fireflies evolved green light luciferase via trade-off of the original function, which was likely aposematic warning display against nocturnal predation.

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Protoluciola albertalleni gen.n., sp.n., a new Luciolinae firefly (Insecta: Coleoptera: Lampyridae) from Burmite amber

Cited by 16 publications

References 3 publications

Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies

Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies

Cloning and Characterization of Luciferase from the Chinese Firefly Lamprigera yunnana

Resurrecting the ancient glow of the fireflies

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