Protocols of proteins interactomics: molecular fishing on optical chips and magnetic nanoparticles

Abstract

Now it is absolutely clear, that the majority of proteins in living systems function due to interaction with each other in stable or dynamic proteins complexes. Therefore necessity of deeper studies of proteins functions causes expansion of protein-protein interaction research. In the present review the brief description and comparative estimation of experimental methods and protocols of protein interactomics, based on technology of molecular fishing on an optical chips and paramagnetic nanoparticles is given.

Mass Spectrometry as a Crucial Analytical Basis for Omics Sciences

“…In this brief review, we only consider those techniques in which the capabilities of MS were manifested to the greatest extent. Currently, there are two mass spectrometric approaches to the study of protein interactions [ 51 , 52 ].…”