Protocols in Advanced Genomics and Allied Techniques

Pal, Aruna

doi:10.1007/978-1-0716-1818-9

Cited by 21 publications

(21 citation statements)

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“…The samples of ovary (n=6) were collected from each group of non-layer (sexually immature or infertile duck, Group I) and highest laying duck (Group II) were collected. The total RNA was isolated from the ovary of Duck by Trizol method and was further used for cDNA synthesis [38][39][40][41][42] .…”

Section: Materials and Methodmentioning

confidence: 99%

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Genomics for reproduction in Anas platyrynchos-a novel report

Debnath

Pal

Chakraborty

et al. 2022

Preprint

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Anas platyrynchos (ducks) are reared mostly for egg, which are very nutritious, that fetch better prices, however duck meat possess rich nutrient content. They possess the unique characteristics of disease resistance to the common avian diseases, even asymptomatic to avian influenza, with a scopeto evolve as one of the best poultry species The major limitation encountered is the lowered average egg production as well as higher age at first egg (an indicator for sexual maturity) for the indigenous ducks compared to that of exotic ones. In this current study, we attempt to explore the genes responsible for duck reproduction in terms of sexual maturity,egg production and fertility of the ducks . We had compared the genomic constitution for the Bengal duck with highest egg production with that of non-layer (infertile or sexually immature) ducks. We characterized the genes in indigenous ducks from ovarian tissues, identified important domains for characterized genes for the first time, and studied differential mRNA expression profiling for these genes with respect to layer and non-layer groups. Upregulation was observed for ESR2, DIAPH2, KMT2E, ASCF2 genes for Bengal duck in highest egg producing duck in comparison to non-layer duck, whereas downregulation was observed for KSR1, A2M, BMPR1B, ACVR1. In the next step, we explored the association with the genes which were actually responsible for egg production. Thus, duck may be utilized as a model for studying the molecular aspect of reproduction. Genes upregulated may be utilized for knock in of gene, whereas down regulated genes may be knocked out or knocked down through gene editing technologies for the improvement of reproductive performance of the duck in future. Molecular biomarkers may be developed with these genes for early selection of better reproducing ducks at day of hatch or even earlier.

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Section: Materials and Methodmentioning

confidence: 99%

“…Each sample was run in duplicate. Analysis of real-time PCR (qRT-PCR) was performed by delta-delta-Ct (ΔΔCt) method 31,2,4,38,39,42 .…”

Section: Methodsmentioning

confidence: 99%

Genomics for reproduction in Anas platyrynchos-a novel report

Debnath

Pal

Chakraborty

et al. 2022

Preprint

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“…To determine virus replication in chickens, paraffin sections of the trachea and lungs were made and the distribution of the virus was observed by immunohistochemical (IHC) assay as described (44). Briefly, tissues from IAV-infected chickens were fixed in phosphatebuffered 10% formalin solution, then paraffin-embedded, sectioned, and incubated for 1h at RT for IAV staining using a nucleoprotein (NP)-specific monoclonal antibody to IAV (prepared by our laboratory).…”

Section: Immunohistochemical Assaymentioning

confidence: 99%

A Single Vaccination of Chimeric Bivalent Virus-Like Particle Vaccine Confers Protection Against H9N2 and H3N2 Avian Influenza in Commercial Broilers and Allows a Strategy of Differentiating Infected from Vaccinated Animals

et al. 2022

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H9N2 and H3N2 are the two most important subtypes of low pathogenic avian influenza viruses (LPAIV) because of their ongoing threat to the global poultry industry and public health. Although commercially available inactivated H9N2 vaccines are widely used in the affected countries, endemic H9N2 avian influenza remains uncontrolled. In addition, there is no available avian H3N2 vaccine. Influenza virus-like particles (VLPs) are one of the most promising vaccine alternatives to traditional egg-based vaccines. In this study, to increase the immunogenic content of VLPs to reduce production costs, we developed chimeric bivalent VLPs (cbVLPs) co-displaying hemagglutinin (HA) and neuraminidase (NA) of H9N2 and H3N2 viruses with the Gag protein of bovine immunodeficiency virus (BIV) as the inner core using the Bac-to-Bac baculovirus expression system. The results showed that a single immunization of chickens with 40μg/0.3mL cbVLPs elicited an effective immune response and provided complete protection against H9N2 and H3N2 viruses. More importantly, cbVLPs with accompanying serological assays can successfully accomplish the strategy of differentiating infected animals from vaccinated animals (DIVA), making virus surveillance easier. Therefore, this cbVLP vaccine candidate would be a promising alternative to conventional vaccines, showing great potential for commercial development.

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“…Ducks were observed to be mostly forager with better disease resistance (Pal et al, 2021), higher adaptability and hardy breed (Pal, A. 2020.…”

Section: Introductionmentioning

confidence: 99%

“…Growth is a polygenic quantitative trait, hence it is important to study certain genes, like mucin gene, affecting gut integrity and nutrient absorption senario. In our lab, we are studying growth parameters including body weight and growth parameters for livestock species (19-21 Pal et al2010, Pal, A, 2020, Pal et al, 2012, Pal et al, 2004, Pal et al, 2017, Pal et al, 2006, Pal et al, 2020. Hence the current study was designed to assess the role of mucin gene for growth of ducks through differential mRNA expression profiling.…”

Section: Introductionmentioning

confidence: 99%

Role of mucin gene for growth in Anas platyrynchos - a novel report

Murmu

Pal

Debnath

et al. 2022

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Mucin gene is expressed at the mucous membrane of the inner layer of the internal organs. Intestinal mucin 2 (MUC2), a major gel-forming mucin, represents a primary barrier component of mucus layers. This is the first report of role of mucin gene in growth traits in animals. In the current study, we had randomly studied Bengal ducks (Anas platyrynchos) reared from day old to 10 weeks of age under organized farm and studied the growth parameters as well as body weight and average daily body weight gain. We had characterized mucin gene for Bengal duck and observed glycosylation and EGF1 (EGF like domain signature) as important domain for growth traits in duck. We observed better expression profile for mucin gene in high growing ducks in comparison to that of lower growing ducks with real time PCR. Hence mucin gene may be employed as a marker for growth traits. Key words: Indigenous duck, Mucin, body weight, biomorphometric characteristics, differential mRNA expression profiling.

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Protocols in Advanced Genomics and Allied Techniques

Cited by 21 publications

References 0 publications

Genomics for reproduction in Anas platyrynchos-a novel report

Genomics for reproduction in Anas platyrynchos-a novel report

A Single Vaccination of Chimeric Bivalent Virus-Like Particle Vaccine Confers Protection Against H9N2 and H3N2 Avian Influenza in Commercial Broilers and Allows a Strategy of Differentiating Infected from Vaccinated Animals

Role of mucin gene for growth in Anas platyrynchos - a novel report

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