Protocol to identify amino acids bound to tRNA by aminoacylation using mass spectrometry

Finkler, Marc; Brandt, Joshua; Boutfol, Timothée; Grimm, Florent; Hartz, Philip; Ott, Albrecht

doi:10.1016/j.xpro.2023.102504

STAR Protocols

2023

DOI: 10.1016/j.xpro.2023.102504

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Protocol to identify amino acids bound to tRNA by aminoacylation using mass spectrometry

Marc Finkler,

Joshua Brandt,

Timothée Boutfol

et al.

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2024

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Strategies for Detecting Aminoacylation and Aminoacyl‐tRNA Editing In Vitro and In Cells

Watkins,

Kavoor,

Musier‐Forsyth

2024

Israel Journal of Chemistry

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Aminoacyl‐tRNA synthetases (aaRSs) maintain translational fidelity by ensuring the formation of correct aminoacyl‐tRNA pairs. Numerous point mutations in human aaRSs have been linked to disease phenotypes. Structural studies of aaRSs from human pathogens encoding unique domains support these enzymes as potential candidates for therapeutics. Studies have shown that the identity of tRNA pools in cells changes between different cell types and under stress conditions. While traditional radioactive aminoacylation analyses can determine the effect of disease‐causing mutations on aaRS function, these assays are not amenable to drug discovery campaigns and do not take into account the variability of the intracellular tRNA pools. Here, we review modern techniques to characterize aaRS activity in vitro and in cells. The cell‐based approaches analyse the aminoacyl‐tRNA pool to observe trends in aaRS activity and fidelity. Taken together, these approaches allow high‐throughput drug screening of aaRS inhibitors and systems‐level analyses of the dynamic tRNA population under a variety of conditions and disease states.

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Strategies for Detecting Aminoacylation and Aminoacyl‐tRNA Editing In Vitro and In Cells

Watkins,

Kavoor,

Musier‐Forsyth

2024

Israel Journal of Chemistry

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Protocol to identify amino acids bound to tRNA by aminoacylation using mass spectrometry

Cited by 1 publication

References 15 publications

Strategies for Detecting Aminoacylation and Aminoacyl‐tRNA Editing In Vitro and In Cells

Strategies for Detecting Aminoacylation and Aminoacyl‐tRNA Editing In Vitro and In Cells

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