Protocol for vegetative propagation of Trillium govanianum Wall ex D. Don

Chauhan, Harsh Kumar; Bisht, Anil Kumar; Bhatt, Indra D.; Bhatt, Arvind

doi:10.1016/j.jarmap.2019.100233

Cited by 8 publications

(3 citation statements)

References 31 publications

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“…PLB formation increased by adding a combination of TDZ and NAA 5μM to MS media [8], while the addition of BA up to 1mg/L, to shoots of Paphiopedilum villosum had the highest yield [9]. Furthermore, the addition of BA 2,5 ppm showed optimal results in the leaf area of Trillium govanianum leaves [10]. The independent effect of NAA and BA and the interactions produced different results measured in the form of numbers of leaves, roots, and shoots and plant height and root length.…”

Section: Resultsmentioning

confidence: 95%

The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro

Hartati¹,

B.A²,

R.H³

et al. 2022

International Journal on Advanced Science, Engineering and Information Technology

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The Orchid is an ornamental plant with high economic value due to the emergence of unique varieties. These varieties are produced from conventional breeding methods of crossing and selection. Meanwhile, the propagation of orchids through in vivo hatcheries is constrained because the seeds are too small to contain the endosperm. Tissue culture is an alternative for the propagation of orchids Efforts to propagate orchids through tissue culture is carried out by adding Growth Regulatory Substances such as auxins groups, namely NAA, IBA, IAA, and cytokinin groups, namely BA, BAP, kinetin, and TDZ to MS media, to enable the plants to take up adequate nutrients for growth. Therefore, this study aims to determine the effect of various concentrations of NAA and BA on the best ½ MS media for the growth of orchid subcultures. A completely randomized design pattern (CRD) of 2 factors with 12 treatment combinations and four replications were used, totaling 48 experimental units. The results showed that the addition of 1ppm NAA had the highest number of roots and length, while the addition of BA at 3ppm had the highest height, and the combination of 1ppm NAA and BA 3ppm treatment produced the highest number of leaves (42 sheets) and shoots. Based on the results, the addition of NAA above 3ppm followed by BA at 6ppm can produce more leaves and shoots, while NAA above 3ppm can produce higher plant height.

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Section: Resultsmentioning

confidence: 95%

The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro

Hartati¹,

B.A²,

R.H³

et al. 2022

International Journal on Advanced Science, Engineering and Information Technology

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“…The limited availability of seeds and the heterogeneous nature of seedlings pose constraints on propagation, despite the plant’s local invasiveness via rhizomes. Although rhizomes and stem cuttings are considered as alternative methods for propagation [ 8 , 12 , 13 , 14 ], they come with inherent limitations in large-scale production. The challenges encompass insufficient material supply, restricted seasonal propagation, and exposure of plant materials to various unfavorable factors, such as pests, pathogens, and drought [ 8 , 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Regeneration from Leaf Explants of Helianthus verticillatus, a Critically Endangered Sunflower

Nowakowska,

Pavlovic,

Nowicki

et al. 2024

Plants

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Helianthus verticillatus (Asteraceae), a whorled sunflower, is a perennial species restricted to a few locations in the southeastern United States and is now considered endangered. Therefore, restoring and protecting H. verticillatus as a species is a priority. This study introduces a highly efficient in vitro adventitious plant regeneration system from leaf explants, utilizing five diverse specimens of H. verticillatus, each representing distinct genotypes with phenotypic variations in leaf and stem morphology. Key factors influencing in vitro morphogenesis, including genetic constitution, explant source, and plant growth regulators (PGRs), were identified. The study revealed a remarkably strong genotype-dependent impact on the regeneration efficiency of the investigated H. verticillatus genotypes, ranging from a lack of regeneration to highly effective regeneration. The selection of two genotypes with varying regeneration abilities provides valuable models for genetic analyses, offering insights into factors influencing the regeneration potential of this endangered species. Optimum adventitious shoot regeneration results were achieved using Murashige and Skoog basal media (MS) supplemented with 8.8 µM N6-benzyladenine (BA) and 1.08 µM α-naphthalene acetic acid (NAA). This combination yielded the highest adventitious shoot production. Subsequent successful rooting on ½ MS medium without PGRs further solidified the efficiency of the developed protocol. Regenerated plantlets, demonstrating robust shoots and roots, were successfully acclimatized to greenhouse conditions with a 95% survival rate. The protocol developed in this study is the first such report for this endangered species and is expected to contribute to future genetic manipulation and modification studies.

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“…Therefore, it is the preferred propagation method for many species [ 12 , 13 ]. For example, vegetative propagation of Trillium govanianum Wall ex D. Don is achieved by positioning rhizomes horizontally in plastic bags containing 50% organic compost, 25% soil, and 25% sand [ 14 ], whereas adventitious stem cuttings of Cycas achieve higher rooting rates with sealer [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

An Efficient Propagation System through Root Cuttings of an Ecological and Economic Value Plant—Broussonetia papyrifera (L.) L’Hér. ex Vent

Zou

Lin

Zhang

et al. 2022

Plants

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Broussonetia papyrifera (L.) L’Hér. ex Vent. has considerable economic and ecological value and a long history of use in China. In this paper, root cuttings were used as the material to establish an efficient vegetative propagation of B. papyrifera. The results revealed that root segments with a diameter of 1.5~2.0 cm and a length of 20~30 cm were most suitable for shoot regeneration, as these segments had the highest adventitious shoot induction rates (93.3%), strongest adventitious shoots, and highest multiplication coefficients (7.07). With regard to the methods used for root burial, a horizontal burial at a depth of 1~3 cm yielded the best results, in this case, the adventitious shoot induction rate can reach 86.7%. The best substrate combination was perlite: peat: coconut chaff = 1:1:1 (v/v/v), wherein the adventitious shoot induction rate can reach 75.6%. The best sterilization method was mixing soil with carbendazim and soaking the root sections in carbendazim for 30 min, wherein the adventitious shoot induction rate can reach 77.8%. Adding 0.2 mg/L naphthaleneacetic acid (NAA) to 1/4 Hoagland nutrient solution significantly improved the rooting rate of adventitious shoots to 82.2%, and the survival rate of the acclimatized plants was more than 90.0%.

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Protocol for vegetative propagation of Trillium govanianum Wall ex D. Don

Cited by 8 publications

References 31 publications

The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro

The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro

In Vitro Regeneration from Leaf Explants of Helianthus verticillatus, a Critically Endangered Sunflower

An Efficient Propagation System through Root Cuttings of an Ecological and Economic Value Plant—Broussonetia papyrifera (L.) L’Hér. ex Vent

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