Protocol for tracking the inheritance patterns of proteins in live Saccharomyces cerevisiae cells using a photo-convertible fluorescent protein

Auboiron, Morgane; Vasseur, Pauline; Radman‐Livaja, Marta

doi:10.1016/j.xpro.2021.100557

Cited by 1 publication

(1 citation statement)

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“…1 ) Using gene technology that can selectively target “old” telomerase RNAs without affecting cell function will be useful for studying these long-lived RNAs. Photo-switchable fluorescent proteins like Dendra2 (Gurskaya et al 2006 ; Auboiron et al 2021 ) could be used in conjunction with RNA–protein binding systems (i.e. MS2-stem-loop:MS2-coat protein binding; Bertrand et al 1998 ; Gallardo et al 2008 ; Laprade et al 2020 ) or by inducible RNA modification as in Vasianovich et al ( 2020 ), enabling specific and long-term analysis of old telomerase RNAs.…”

Section: Telomerase Rnasmentioning

confidence: 99%

Methods that shaped telomerase research

Bartle,

Wellinger

2023

Biogerontology

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Telomerase, the ribonucleoprotein (RNP) responsible for telomere maintenance, has a complex life. Complex in that it is made of multiple proteins and an RNA, and complex because it undergoes many changes, and passes through different cell compartments. As such, many methods have been developed to discover telomerase components, delve deep into understanding its structure and function and to figure out how telomerase biology ultimately relates to human health and disease. While some old gold-standard methods are still key for determining telomere length and measuring telomerase activity, new technologies are providing promising new ways to gain detailed information that we have never had access to before. Therefore, we thought it timely to briefly review the methods that have revealed information about the telomerase RNP and outline some of the remaining questions that could be answered using new methodology.

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