Protocol for Somatic Embryogenesis in Japanese Black Pine (Pinus thunbergii Parl.) and Japanese Red Pine (Pinus densiflora Sieb. et Zucc.)

Maruyama, Tsuyoshi; Hosoi, Yoshihisa

doi:10.1007/978-3-319-89483-6_17

Cited by 8 publications

(28 citation statements)

References 30 publications

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“…The aim of partial desiccation of somatic embryos is to reduce the water content and/or to complete the maturation process (Roberts et al, 1990). However, partial desiccation is not necessary if the water content of somatic embryos is adequately low at the end of the maturation period, e.g., when somatic embryos are matured on media containing high concentrations of GG (Klimaszewska and Cyr, 2002; Maruyama and Hosoi, 2016).…”

Section: General Consideration On Se In Pinesmentioning

confidence: 99%

“…The low initiation frequency of SE, low number of embryos generated, and low frequency of plant conversion are the leading problems that hamper the efficient large-scale production of some species and limit their widespread practical uses. The efficient production of high-quality somatic embryos is the most important criterion for using SE protocols in commercial propagation and tree improvement programs (Maruyama and Hosoi, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…(Ryukyu pine) are important native species in Japan used for reforestation (Maruyama and Hosoi, 2014). Japanese black pine is also important for protection of the coastal areas, and Japanese red pine is the principal host species of the prized “matsutake mushroom” (Maruyama and Hosoi, 2016). Ryukyu pine is valued for construction of houses and furnishings (Hosoi and Maruyama, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Since its introduction into Japan from North America, the pine wilt disease has rapidly spread to China, Korea, and Taiwan (Togashi and Shigesada, 2006) and also has devastated pine forests in Portugal, Spain and other European countries (Mota et al, 1999; Nunes da Silva et al, 2015). Therefore, it is essential to establish a practical and effective plant regeneration method for mass propagation of resistant clones (Maruyama and Hosoi, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…This review describes progress in SE of four species of Japanese pines (JPs) over the last decade, focusing on the two protocols most commonly reported for plant production in Pinus species through to SE (Maruyama and Hosoi, 2016). Somatic plant regeneration from maturation protocols using polyethylene glycol (PEG) or gellan gum (GG) at a high concentration are compared, and the positive effect of somatic embryo desiccation after PEG-mediated maturation is emphasized (Maruyama and Hosoi, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Progress in Somatic Embryogenesis of Japanese Pines

Maruyama

Hosoi

2019

Front. Plant Sci.

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Somatic embryogenesis (SE) in not only one of the most promising techniques for mass propagation of selected trees, but also is a valuable tool for basic research studies in cell biology and genetic engineering, and it allows the long-term ex situ conservation of genetic resources by cryopreservation techniques. This review reports the most recent progress in SE, protoplast culture, and cryopreservation of four important Japanese pines (Pinus thunbergii, Pinus densiflora, Pinus armandii var. amamiana, and Pinus luchuensis). Induction of embryogenic tissues (ET), embryogenic culture maintenance/proliferation, production of somatic embryos, germination, and conversion to plants are described focusing on the protocols most commonly reported for plant production in Pinus species through to SE.

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Section: General Consideration On Se In Pinesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Progress in Somatic Embryogenesis of Japanese Pines

Maruyama

Hosoi

2019

Front. Plant Sci.

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Somatic embryogenesis of slash pine (Pinus elliottii Engelm.): initiation, maturation, germination and mycorrhization of regenerated plantlets

Fei,

Chen,

et al. 2024

Plant Cell Tiss Organ Cult

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Slash pine (Pinus elliottii Engelm.), an important economic tree species with a high resin yield, is extensively cultivated in southern China. Somatic embryogenesis (SE) technology could hasten the breeding of P. elliottii through improving seedling quality and number, but mass production of somatic embryos remains constrained by various factors. We described an efficient SE system through indirect route and investigate the effects of genotype, phytohormones and culture condition on SE. Immature zygotic embryos of three open-pollinated mother trees collected during 2020-2023 were utilized for initiation of embryogenic callus (EC). The initiation of EC was significantly influenced by seed sources (families) (p < 0.05). The addition of 2,4-dichlorophenoxyacetic acid (6 mg/L), 6-benzylaminopurine (1 mg/L) and kinetin (2 mg/L) effectively improved initiation rates by up to 15.67 ± 1.89%. Furthermore, glutamine addition to the maturation medium enhanced the yield of SE during culture. The optimum concentration range of activated carbon within the germination medium spanned from 3-4.5 g/L. Inoculation with ectomycorrhizal fungus Laccaria amethystea enhanced the root length of regenerated plantlets, reaching 7.92 ± 4.06 cm. The regenerated plantlets inoculated with mycorrhizae Pisolithus orientalis and Xerocomus chrysenteron showed improved survival during the acclimatization phase, with 100% survival of mycorrhizae-treated plantlets after 3 months of acclimatization in the greenhouse. We clarified the SE conditions suitable for P. elliottii, and the SE system and mycorrhizal method we used can be applied to mass production of plantlets.

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Evaluation of somatic embryo production during embryogenic tissue proliferation stage using morphology, maternal genotype, proliferation rate and tissue age of Pinus thunbergii Parl

et al. 2021

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To determine the optimal embryogenic capacity (somatic embryo production) of the selected elite nematode-resistant genotypes of Pinus thunbergii, variables such as embryogenic tissue (ET) morphology, maternal genotype, proliferation rate and tissue age were analyzed. ET morphology and histological evaluation of the proliferation stage showed a decrease in filamentous clump and protuberant surfaces and a decline in the acetocarmine-staining area, which indicates a decrease in somatic embryo production (SEP). Variations in cell physiology during the proliferation stage showed that SEP was positively correlated with soluble sugars and proteins, but negatively correlated with starch, peroxidase, and superoxidase. In addition, SEP was significantly (p < 0.001) affected by maternal genotype, tissue age and proliferation rate. Moreover, SEP was positively correlated with proliferation rate (r = 0.98, p < 0.001), but negatively correlated with tissue age (r = − 0.95, p < 0.001). In general, the results suggest that SEP could be assessed in ET proliferation stages by the apparent cell morphology, histology, proliferation rate and tissue age, which provides novel insights for evaluating the ET maturation capacity (number of somatic embryos) during the proliferation stage of P. thunbergii somatic embryogenesis.

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Protocol for Somatic Embryogenesis in Japanese Black Pine (Pinus thunbergii Parl.) and Japanese Red Pine (Pinus densiflora Sieb. et Zucc.)

Cited by 8 publications

References 30 publications

Progress in Somatic Embryogenesis of Japanese Pines

Progress in Somatic Embryogenesis of Japanese Pines

Somatic embryogenesis of slash pine (Pinus elliottii Engelm.): initiation, maturation, germination and mycorrhization of regenerated plantlets

Evaluation of somatic embryo production during embryogenic tissue proliferation stage using morphology, maternal genotype, proliferation rate and tissue age of Pinus thunbergii Parl

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