Protocol for multiplex fluorescent immunohistochemistry in free-floating rodent brain tissues

Kelly, Aubrey M.; Fricker, Brandon A.; Wallace, Kelly J.

doi:10.1016/j.xpro.2022.101672

Cited by 3 publications

(1 citation statement)

References 2 publications

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“…compound and stored at − 80 °C before sectioning coronally at 40 µm using a Leica cryostat, with every third section saved for use in the present study. Tissue sections were immunofluorescently stained for OT and VP following previously established protocols 60 . Tissue was rinsed 5× for 10 min in 0.1 M PBS (pH 7.4), incubated for 1 h in a blocking solution (PBS + 10% normal donkey serum + 0.03% Triton-X-100) to prevent non-specific binding, and then incubated for approximately 48 h in primary antibodies diluted in PBS containing 5% normal donkey serum + 0.03% Triton-X-100.…”

Section: Partner Preference Testmentioning

confidence: 99%

Partner separation rescues pair bond-induced decreases in hypothalamic oxytocin neural densities

Fricker

Roshko

Jiang

et al. 2023

Sci Rep

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Studies in prairie voles (Microtus ochrogaster) have shown that although formation of the pair bond is accompanied by a suite of behavioral changes, a bond between two voles can dissolve and individuals can form new pair bonds with other conspecifics. However, the neural mechanisms underlying this behavioral flexibility have not been well-studied. Here we examine plasticity of nonapeptide, vasopressin (VP) and oxytocin (OT), neuronal populations in relation to bonding and the dissolution of bonds. Using adult male and female prairie voles, animals were either pair bonded, co-housed with a same-sex sibling, separated from their pair bond partner, or separated from their sibling. We examined neural densities of VP and OT cell groups and observed plasticity in the nonapeptide populations of the paraventricular nucleus of the hypothalamus (PVN). Voles that were pair bonded had fewer PVN OT neurons, suggesting that PVN OT neural densities decrease with pair bonding, but increase and return to a pre-pair bonded baseline after the dissolution of a pair bond. Our findings suggest that the PVN nonapeptide cell groups are particularly plastic in adulthood, providing a mechanism by which voles can exhibit context-appropriate behavior related to bond status.

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Section: Partner Preference Testmentioning

confidence: 99%