Protocol for detecting histidine polyphosphate modification of human proteins via MBP-tagged expression in E. coli

Lehotsky, Kirsten; Neville, Nolan; Jia, Zongchao

doi:10.1016/j.xpro.2024.102947

STAR Protocols

2024

DOI: 10.1016/j.xpro.2024.102947

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Protocol for detecting histidine polyphosphate modification of human proteins via MBP-tagged expression in E. coli

Kirsten Lehotsky,

Nolan Neville,

Zongchao Jia

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2024

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Cited by 2 publications

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Polyphosphate attachment to lysine repeats is a non-covalent protein modification

Neville,

Lehotsky,

Klupt

et al. 2024

Molecular Cell

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Polyphosphate attachment to lysine repeats is a non-covalent protein modification

Neville,

Lehotsky,

Klupt

et al. 2024

Molecular Cell

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His-Tagging: Exploring Precise Chemical Modification of Histidine-Containing Bioactive Peptide Sequences

Chatterjee,

Paul,

Shanker

2024

Synlett

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The modification of biomolecules, particularly peptides, has garnered considerable attention from researchers, effectively serving as a connection between chemistry and biology. The modification of peptides can facilitate, among others, the generation of peptide drugs, antibody–drug conjugates, and probes for molecular imaging. Herein, we have carefully curated reactions and chemical transformations of bioactive peptide sequences equipped with histidine amino acids that are conducive for biological applications. This Account also highlights strategies for the chemical modification of histidine that might capture the imagination of both peptide researchers and synthetic chemists.1 Introduction2 Histidine Modification in Bioactive Peptides and Proteins3 Remote Bioactive Peptides and Protein Modification Adjacent to Histidine4 Conclusions and Future Directions

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Protocol for detecting histidine polyphosphate modification of human proteins via MBP-tagged expression in E. coli

Cited by 2 publications

References 5 publications

Polyphosphate attachment to lysine repeats is a non-covalent protein modification

Polyphosphate attachment to lysine repeats is a non-covalent protein modification

His-Tagging: Exploring Precise Chemical Modification of Histidine-Containing Bioactive Peptide Sequences

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