Protocol for assessing ex vivo lipolysis of murine adipose tissue

T cells with increased responsiveness cause obesity in mice without diet intervention

“…Viceral, epidydymal adipose depots were dissected from 3 month old RIAD mice (n=5) and control mice (n=5) to assess ex vivo lipolysis of visceral adipose tissue, as previously described by Roy et al., 25 with minor adjustments. Briefly, ∼50 mg of adipose depots were incubated in KRBH buffer with or without isoproterenol for 2 hours.…”

Adipose tissue peroxisomal lipid synthesis orchestrates obesity and insulin resistance through LXR-dependent lipogenesis

“…An ex vivo lipolysis assay was performed based on the protocol presented in [ 17 ]. Briefly, 50–100 mg chunks of gWAT tissue were harvested from control and HFD-fed PexRAP-AKO mice and immediately transferred to prewarmed (37 °C) KRBH buffer in 12-well plates.…”

α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury

“…Lipolytic activity in white adipose tissue was performed as described elsewhere. 87 Briefly, eWAT and iWAT (left side) were isolated in Krebs-Ringer bicarbonate buffer (KRBH) (30 mM HEPES, 120 mM NaCl, 1 mM CaCl 2 , 1 mM MgCl 2 , 10mM NaHCO 3 , 4 mM K 2 HPO 4 ) prewarmed at 37°C. Adipose tissue was divided into 30–50 mg specimens and incubated at 37°C in KRBH buffer supplemented with 2% fatty acid-free BSA (A7030, Sigma) with either vehicle (sterile dH 2 O) or 10 μM isoproterenol (ab146724, Abcam) for 2 h with constant agitation.…”