Protic ionic liquid-assisted cell disruption and lipid extraction from fresh water Chlorella and Chlorococcum microalgae

Shankar, Mukund; Chhotaray, Pratap K.; Agrawal, Ayushi; Gardas, Ramesh L.; Tamilarasan, K.; Rajesh, Mathur

doi:10.1016/j.algal.2017.05.009

Cited by 45 publications

(10 citation statements)

References 30 publications

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“…However, it was in the biomass grown in the pilot-scale PBR that the highest percentage of carbohydrates was detected, achieving 17.43% of biomass DW (p < 0.05), within the range of reported values for the majority of microalgae [4,9]. Indeed, under unfavorable conditions (e.g., high irradiance and nitrogen limitation), the excess of carbon fixed during photosynthesis may have been diverted into storage compounds such as lipids or carbohydrates [4,9,37,38]. This could also explain the higher lipid content observed in the biomass grown in the 10-m 3 PBR (p < 0.05), 31.44% DW, in comparison to that of microalgal cells grown in the 2.5-m 3 PBR (18.33%).…”

Section: Proximate Compositionsupporting

confidence: 66%

Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond

Correia

Pereira

Silva³

et al. 2020

Applied Sciences

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Bioprospection of novel autochthonous strains is key to the successful industrial-scale production of microalgal biomass. A novel Chlorococcum strain was recently isolated from a pond inside the industrial production facility of Allmicroalgae (Leiria, Portugal). Phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences suggests that this isolate is a novel, free-living Oophila amblystomatis strain. However, as our phylogenetic data strongly suggests that the aforementioned taxon belongs to the genus Chlorococcum, it is here proposed to rename this species as Chlorococcum amblystomatis. In order to characterize the biotechnological potential of this novel isolate, growth performance and biochemical composition were evaluated from the pilot (2.5-m3) to industrial (10-m3) scale. The highest maximum areal productivity (36.56 g·m−2·day−1) was reached in a 10-m3 tubular photobioreactor (PBR), as compared to that obtained in a 2.5-m3 PBR (26.75 g·m−2·day−1). Chlorococcum amblystomatis displayed high protein content (48%–56% dry weight (DW)) and moderate levels of total lipids (18%–31% DW), carbohydrates (6%–18% DW) and ashes (9%–16% DW). Furthermore, the lipid profile was dominated by polyunsaturated fatty acids (PUFAs). The highest pigment contents were obtained in the 2.5-m3 PBR, where total chlorophylls accounted for 40.24 mg·g−1 DW, followed by lutein with 5.37 mg·g−1 DW. Overall, this free-living Chlorococcum amblystomatis strain shows great potential for nutritional applications, coupling a promising growth performance with a high protein content as well as relevant amounts of PUFAs, chlorophyll, and carotenoids.

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Section: Proximate Compositionsupporting

confidence: 66%

Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond

Correia

Pereira

Silva³

et al. 2020

Applied Sciences

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“…Ionic liquids (ILs) have been shown to increase the lipid extraction efficiency from microalgae by dissolving both dry and wet microalgae under relatively mild conditions (80–140 °C) with and without a co-solvent ( Table 1 ) [ 1 , 30 , 31 ]. ILs are often described as “green” designer solvents with many desirable physical-chemical properties such as great thermal stability, low melting points, and negligible vapor pressure [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Efficient Extraction of a Docosahexaenoic Acid (DHA)-Rich Lipid Fraction from Thraustochytrium sp. Using Ionic Liquids

et al. 2018

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Polyunsaturated fatty acids (PUFAs) play a significant role in the modulation and prevention of various diseases, and hence are attracting increasing attention from the biotech industry. Thraustochytrids are marine heterokonts that exhibit robust growth rates, high PUFA content, and more specifically, a large percentage of omega-3 fatty acids like docosahexaenoic acid (DHA). Recently, ionic liquids (ILs) have been shown to improve the efficiency of organic solvent extraction of oils from wet oleaginous yeast and microalgae under mild conditions. Two ILs, the imidazolium 1-ethyl-3-methylimidazolium ethylsulfate [C2mim][EtSO4] IL and the phosphonium (tetrabutylphosphonium propanoate [P4444][Prop]) IL were assessed for their ability to facilitate extraction of PUFA-containing lipids from a Thraustochytrium sp. (T18) through efficient cell wall disruption. The oil extracted after IL pretreatment was further characterized with respect to fatty acid methyl ester (FAME) composition, while the effects of process parameters, such as the ratio of ionic liquid to co-solvent, the mass ratio of microalgae to the mixture of ionic liquid, and type of co-solvent were also investigated for both ILs. The results indicate that these ILs can disrupt the cells of Thraustochytrium sp. when mixed with a co-solvent (methanol), and facilitated the recovery of oils over a large degree of dewatered Thraustochytrium biomass (0–77.2 wt% water) in a short period of time (60 min) at ambient temperature, hence demonstrating a water compatible, low-energy, lipid recovery method. The lipid recovery was not affected by repeated usage of recycled ILs (tested up to five times).

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“…The best results were demonstrated by butyrolactam hexanoate with efficiency of 76% and 84% for Chlorella sp. and Chlorococcum sp., respectively (Shankar et al, 2017). These results were obtained at room temperature, but long treatment times of 24 hours were required.…”

Section: Ionic Liquidsmentioning

confidence: 71%

Current and novel approaches to downstream processing of microalgae: A review

Nitsos

Filali

Taidi

et al. 2020

Biotechnology Advances

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Protic ionic liquid-assisted cell disruption and lipid extraction from fresh water Chlorella and Chlorococcum microalgae

Cited by 45 publications

References 30 publications

Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond

Isolation, Identification and Biotechnological Applications of a Novel, Robust, Free-living Chlorococcum (Oophila) amblystomatis Strain Isolated from a Local Pond

Efficient Extraction of a Docosahexaenoic Acid (DHA)-Rich Lipid Fraction from Thraustochytrium sp. Using Ionic Liquids

Current and novel approaches to downstream processing of microalgae: A review

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