Prothrombin ? The substrate for blood plasma kallikrein and factor XIIa

Baskova, I. P.; Tuero, A.; Krizhevskaya, Yu. V.; Yarovaya, G. A.; Mirkamalova, �. G.

doi:10.1007/bf00839816

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“…The basal amidolytic activity in the 10 μg/mL kallikrein incubation might be due to cleavage of the thrombin substrate by thrombin amounts generated by action of this high concentration of kallikrein on prothrombin in spite of presence of arginine. An interaction of kallikrein with prothrombin has already been found by Baskova et al 16 However, this group did not describe the activation of prothrombin by kallikrein, possibly because their assay conditions did not try to imitate the physiology, and their test system was not able to detect trace amounts of thrombin.…”

Section: Brief Reportsmentioning

confidence: 92%

Kallikrein Activates Prothrombin

Stief

2008

Clin Appl Thromb Hemost

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Kallikrein is a multitalented enzyme in hemostasis and inflammation. Normally, kallikrein is formed in intrinsic hemostasis and activates factor XII. A total of 10 µL of 0 to 100 µg/mL human plasma kallikrein in 6% human albumin—PBS were incubated with 90 µL 111.1 µg/mL prothrombin in 6% human albumin in absence and presence of 23 mM Ca++. After 0 to 64 minutes (37°C), 100 µL of 2.5 M arginine, pH 9, were added. Fifty microliters of 0.72 mM HD-CHG-Ala-Arg-pNA in 1.36 M arginine were added and increase in absorbance at 405 nm was determined. Within 8 minutes (37°C), 1 µg/mL kallikrein, ie, 2.5% of the normal plasmatic prekallikrein concentration, generates approximately 3 mIU/mL thrombin in absence and 27 mIU/mL thrombin in presence of Ca++. Kallikrein can directly activate prothrombin; there is a shortcut in the intrinsic hemostasis system that generates catalytic amounts of thrombin without following the known intrinsic clotting pathway.

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Section: Brief Reportsmentioning

confidence: 92%

Kallikrein Activates Prothrombin

Stief

2008

Clin Appl Thromb Hemost

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show abstract

Prothrombin ? The substrate for blood plasma kallikrein and factor XIIa

Cited by 1 publication

References 5 publications

Kallikrein Activates Prothrombin

Kallikrein Activates Prothrombin

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