Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis

Zou, Qianxing; Yang, Lele; Shi, Ruona; Qi, Yuling; Zhang, Xiaofei; Qi, Huayu

doi:10.1016/j.isci.2021.103396

Cited by 17 publications

(25 citation statements)

References 104 publications

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“… (B) A representative image of flow cytometry of spermatogenic cells using Hoechst 33342 staining. Image adapted from ( Zou et al., 2021 ). (C and D) (C) Representative images of flow cytometry of spermatogonial stem cells.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… (F) Representative confocal images of immunocytochemistry and Giemsa staining showing spermatocytes at different meiotic stages. Images adapted and modified from ( Zou et al., 2021 ). …”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… (B) A representative image of flow cytometry of spermatogenic cells using Hoechst 33342 staining. Image adapted from ( Zou et al., 2021 ). …”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… (E) Representative western blotting of puromycin labeled testicular cells. The image is adapted from ( Zou et al., 2021 ). …”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…The isolated cells can be used for analyzing nascent protein synthesis and protein degradation, two main events that maintain cellular proteostasis important for healthy and long-term production of male gametes. For complete details on the use and execution of this protocol, please refer to Zou et al. (2021) .…”

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confidence: 99%

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Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse

Zou

Yang

2022

STAR Protocols

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Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… (F) Representative confocal images of immunocytochemistry and Giemsa staining showing spermatocytes at different meiotic stages. Images adapted and modified from ( Zou et al., 2021 ). …”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… (B) A representative image of flow cytometry of spermatogenic cells using Hoechst 33342 staining. Image adapted from ( Zou et al., 2021 ). …”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… (E) Representative western blotting of puromycin labeled testicular cells. The image is adapted from ( Zou et al., 2021 ). …”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

mentioning

confidence: 99%

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Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse

Zou

Yang

2022

STAR Protocols

Self Cite

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RpS25 is required for sperm elongation and individualization during Drosophila spermatogenesis

Xu,

Pan,

Fang

et al. 2024

Biochemical and Biophysical Research Communications

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Multigenerational Effect of Heat Stress on the Drosophila melanogaster Sperm Proteome

Khan,

Mishra

2024

J. Proteome Res.

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The effect of the parental environment on offspring through non-DNA sequence-based mechanisms, such as DNA methylation, chromatin modifications, noncoding RNAs, and proteins, could only be established after the conception of “epigenetics”. These effects are now broadly referred to as multigenerational epigenetic effects. Despite accumulating evidence of male gamete-mediated multigenerational epigenetic inheritance, little is known about the factors that underlie heat stress-induced multigenerational epigenetic inheritance via the male germline in Drosophila. In this study, we address this gap by utilizing an established heat stress paradigm in Drosophila and investigating its multigenerational effect on the sperm proteome. Our findings indicate that multigenerational heat stress during the early embryonic stage significantly influences proteins in the sperm associated with translation, chromatin organization, microtubule-based processes, and the generation of metabolites and energy. Assessment of life-history traits revealed that reproductive fitness and stress tolerance remained unaffected by multigenerational heat stress. Our study offers initial insights into the chromatin-based epigenetic mechanisms as a plausible means of transmitting heat stress memory through the male germline in Drosophila. Furthermore, it sheds light on the repercussions of early embryonic heat stress on male reproductive potential. The data sets from this study are available at the ProteomeXchange Consortium under the identifier PXD037488.

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Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis

Cited by 17 publications

References 104 publications

Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse

Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse

RpS25 is required for sperm elongation and individualization during Drosophila spermatogenesis

Multigenerational Effect of Heat Stress on the Drosophila melanogaster Sperm Proteome

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