Proteopedia entry: G‐protein coupled receptors

Abstract

Metabolic G-protein Coupled Receptors (GPCRs). (A) Intramembrane access to the binding pocket of GPR40 (also known as free fatty acid receptor 1; PDB code: 4PHU). The binding pocket of GPR40 (grey) is covered by extracellular loop 2 (ECL2; cyan) preventing entry from the extracellular space. Instead the allosteric regulator, TAK-875 (pink), accesses the binding pocket through the plasma membrane. (B) Structural determination of the lysophosphatidic acid receptor (LPA 1 ; PDB code: 4Z34). LPA 1 was crystallized with a stabilizing Cytochrome b 562 RIL subunit (circled in orange) inserted into the third intracellular loop and with membrane lipids bound to help orient LPA 1 in the plasma membrane. (C) Pharmacological regulation of metabotropic glutamate receptor 5 (mGlu5; PDB code: 4OO9). Slab view of the allosteric binding site (allosteric regulator mavoglurant (red)) within the 7-transmembrane helices of mGlu5 (green).

A practical guide to teaching withProteopedia

“…For this course, Proteopedia is a central instructional component, as students applied their protein structure knowledge to complex biochemical systems. In a weekly 2‐h computational lab, students developed Proteopedia pages relating the structure and function of a protein family that reinforced the lecture material for Biochemistry II 25–27 . Over 6 weeks, students worked in groups to learn about the design of a Proteopedia page (week 1), to prepare rough drafts of their Proteopedia pages (weeks 2–4), and to finalize their Proteopedia pages (weeks 5–6) (see Appendix S6).…”

“…For lesson W‐LT, finished presentations included a quiz‐worthy question that could be used later for an online quiz for the rest of the class to engage with the structure and scenes to answer the question. Ways of disseminating student work could be a presentation, involve moving an excellent page from a sandbox to a public page on Proteopedia (some are featured in [Student Projects]), or even publishing in a peer‐reviewed journal (for example lesson W‐JJ, 26 ). This allows students an opportunity to join the community of scholars through a course‐based activity.…”