Proteomics study reveals the molecular mechanisms underlying water stress tolerance induced by Piriformospora indica in barley

Ghabooli, Mehdi; Khatabi, Behnam; Ahmadi, Farajollah Shahriari; Sepehri, Mozhgan; Mirzaei, Mehdi; Amirkhani, Ardeshir; Jorrín‐Novo, Jesús V.; Salekdeh, Ghasem Hosseini

doi:10.1016/j.jprot.2013.09.017

Cited by 162 publications

(111 citation statements)

References 44 publications

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“…Plants being sessile acclimate to environmental cues by undergoing many metabolic changes, one of them being increased protein turnover that includes both protein biosynthesis and ubiquitination (Kosová et al, 2014). Proteomic studies revealed variations in the levels of translation-related proteins such as initiation factors, elongation factors, and proteins of both small and large subunits during the process of acclimation particularly, to dehydration, salt and temperature stresses in cereals (Fatehi et al, 2012; Budak et al, 2013; Ghabooli et al, 2013; Gharechahi et al, 2014). …”

Section: Discussionmentioning

confidence: 99%

Rice Ribosomal Protein Large Subunit Genes and Their Spatio-temporal and Stress Regulation

et al. 2016

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Ribosomal proteins (RPs) are well-known for their role in mediating protein synthesis and maintaining the stability of the ribosomal complex, which includes small and large subunits. In the present investigation, in a genome-wide survey, we predicted that the large subunit of rice ribosomes is encoded by at least 123 genes including individual gene copies, distributed throughout the 12 chromosomes. We selected 34 candidate genes, each having 2–3 identical copies, for a detailed characterization of their gene structures, protein properties, cis-regulatory elements and comprehensive expression analysis. RPL proteins appear to be involved in interactions with other RP and non-RP proteins and their encoded RNAs have a higher content of alpha-helices in their predicted secondary structures. The majority of RPs have binding sites for metal and non-metal ligands. Native expression profiling of 34 ribosomal protein large (RPL) subunit genes in tissues covering the major stages of rice growth shows that they are predominantly expressed in vegetative tissues and seedlings followed by meiotically active tissues like flowers. The putative promoter regions of these genes also carry cis-elements that respond specifically to stress and signaling molecules. All the 34 genes responded differentially to the abiotic stress treatments. Phytohormone and cold treatments induced significant up-regulation of several RPL genes, while heat and H2O2 treatments down-regulated a majority of them. Furthermore, infection with a bacterial pathogen, Xanthomonas oryzae, which causes leaf blight also induced the expression of 80% of the RPL genes in leaves. Although the expression of RPL genes was detected in all the tissues studied, they are highly responsive to stress and signaling molecules indicating that their encoded proteins appear to have roles in stress amelioration besides house-keeping. This shows that the RPL gene family is a valuable resource for manipulation of stress tolerance in rice and other crops, which may be achieved by overexpressing and raising independent transgenic plants carrying the genes that became up-regulated significantly and instantaneously.

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Section: Discussionmentioning

confidence: 99%

Rice Ribosomal Protein Large Subunit Genes and Their Spatio-temporal and Stress Regulation

et al. 2016

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“…Previous studies have reported that some components in the protein degradation pathway, such as ubiquitin/26S proteasomes, small ubiquitin-like modifier (E3 SUMO) ligase, and proteases/peptidases were involved in plant drought tolerance [119,120,121,122,123]. For the ubiquitin/26S proteasome system, 7 out of 11 20S proteasomes (the core regulatory particle of 26S proteasome) were increased in leaves of P. vulgaris [49], Hordeum vulgare [26], B. napus [47], and Medicago sativa [50], respectively. Importantly, the phosphorylation level of E3 ubiquitin ligase, which is one of the key enzymes involved in ubiquitination, exhibited significantly increased values in leaves under drought stress [40,44].…”

Section: Drought-responsive Protein Synthesis and Turnovermentioning

confidence: 99%

“…In response to drought, the translationally-controlled tumor protein homolog (TCTP) was significantly drought-increased in H. vulgare [26], T. aestivum [37], and B. napus [47], which would facilitate plant adaptation to drought stress. TCTP is a Ca 2+ -binding protein with important functions in some cellular processes (e.g., protection against stress and apoptosis, cell growth, cell cycle progression, and microtubule organization) [169].…”

Section: Modulation Of Cell Structure and Cell Cyclementioning

confidence: 99%

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

Wang

Cai

et al. 2016

IJMS

210

143

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Plant drought tolerance is a complex trait that requires a global view to understand its underlying mechanism. The proteomic aspects of plant drought response have been extensively investigated in model plants, crops and wood plants. In this review, we summarize recent proteomic studies on drought response in leaves to reveal the common and specialized drought-responsive mechanisms in different plants. Although drought-responsive proteins exhibit various patterns depending on plant species, genotypes and stress intensity, proteomic analyses show that dominant changes occurred in sensing and signal transduction, reactive oxygen species scavenging, osmotic regulation, gene expression, protein synthesis/turnover, cell structure modulation, as well as carbohydrate and energy metabolism. In combination with physiological and molecular results, proteomic studies in leaves have helped to discover some potential proteins and/or metabolic pathways for drought tolerance. These findings provide new clues for understanding the molecular basis of plant drought tolerance.

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“…The ability of P. indica to improve plant tolerance to abiotic stress has been reported for several plant species (Waller et al, 2005;Baltruschat et al, 2008;Sherameti et al, 2008a;Sun et al, 2010;Alikhani et al, 2013;Ghabooli et al, 2013); however, the basis of this phenomenon is not clear. In all previous studies, plants have been exposed to stress after being precultivated with P. indica for several weeks and probably have acquired benefits from mutualism before stress treatment.…”

Section: Introductionmentioning

confidence: 99%

Sustained exposure to abscisic acid enhances the colonization potential of the mutualist fungus Piriformospora indica on Arabidopsis thaliana roots

et al. 2015

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SummaryRoot colonization by the beneficial fungus Piriformospora indica is controlled by plant innate immunity, but factors that channel this interaction into a mutualistic relationship are not known. We have explored the impact of abscisic acid (ABA) and osmotic stress on the P. indica interaction with Arabidopsis thaliana.The activation of plant innate immunity in roots was determined by measuring the concentration of the phytoalexin camalexin and expression of transcription factors regulating the biosynthesis of tryptophan-related defence metabolites. Furthermore, the impact of the fungus on the content of ABA, salicylic acid, jasmonic acid (JA) and JA-related metabolites was examined.We demonstrated that treatment with exogenous ABA or the ABA analogue pyrabactin increased fungal colonization efficiency without impairment of plant fitness. Concomitantly, ABA-deficient mutants of A. thaliana (aba1-6 and aba2-1) were less colonized, while plants exposed to moderate stress were more colonized than corresponding controls. Sustained exposure to ABA attenuated expression of transcription factors MYB51, MYB122 and WRKY33 in roots upon P. indica challenge or chitin treatment, and prevented an increase in camalexin content.The results indicate that ABA can strengthen the interaction with P. indica as a consequence of its impact on plant innate immunity. Consequently, ABA will be relevant for the establishment and outcome of the symbiosis under stress conditions.

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Proteomics study reveals the molecular mechanisms underlying water stress tolerance induced by Piriformospora indica in barley

Cited by 162 publications

References 44 publications

Rice Ribosomal Protein Large Subunit Genes and Their Spatio-temporal and Stress Regulation

Rice Ribosomal Protein Large Subunit Genes and Their Spatio-temporal and Stress Regulation

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

Sustained exposure to abscisic acid enhances the colonization potential of the mutualist fungus Piriformospora indica on Arabidopsis thaliana roots

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