Proteomics Strategies in Cardiovascular Research

Abstract

Cardiovascular research of the past decades dealt with classical pathophysiological descriptions, then shifted toward the identification of relevant receptors, and then proceeded to the analysis of signal transduction pathways. Most recently, hand in hand with the achievements of the human genome project, the research has gone down the road toward molecular biological "disease gene(s) mapping". The application of proteome research will attempt to close the gap between genomic (and genetic) analysis and the physiological research. The rich source of heart surgery specimens represents an excellent starting point in data acquisition of proteomic context. Furthermore, animal models of cardiovascular diseases and deficiencies are considered, and will be explored. Examples of results from feasibility studies are given, with the emphasis on quantitative evaluation of proteomic components, hoping to discover co-regulated sets of proteins that are involved in any particular disease state. Identification of new, not yet discovered proteins will be pursued, though the emphasis of this work will be on the definition of characteristic sets of expressed proteins, which in turn might be able to delimit the state of disease and prognosis of therapy outcome. Besides the systematic issues, this paper refers to a number of methodological questions, like the comparison of the proteins detected by staining procedures and proteins detected in models in which biosynthetic labeling is applicable.

Proteomic analysis of left ventricular tissues following intermittent myocardial ischemia during coronary collateralization in rabbits

“…More recently, the emerging proteomics involving qualitative alterations in proteins along with the quantitative changes in protein expression may provide new insights into the specific molecular mechanisms underlying ischemic responses [17]. The present study employed an established rabbit model of controlled myocardial ischemia to simulate intermittent ischemic phenomenon during which time cardiac rehabilitation training occurs.…”

Proteome and system ontology of hemorrhagic shock: Exploring early constitutive changes in postshock mesenteric lymph

“…Proteomics studies the panel of proteins present in a cell, organ, or organism at a given time, 47 and whereas the genome reflects what can happen in a given circumstance, the proteome reflects what actually happens. 48 The physiologic response to trauma has been inferred by many specific proteins, which include acute phase components, cytokines, and contents of cellular lysates.…”

Pilot Proteomic Profile of Differentially Regulated Proteins in Right Atrial Appendage Before and After Cardiac Surgery Using Cardioplegia and Cardiopulmonary Bypass

“…Building on the present findings will include analysis of a greater amount of modified proteins as well as significantly larger patient populations to yield an overall picture of CP/CPB-induced changes in the proteome. It is our hope this analysis will facilitate: (1) improved cardioprotection strategies (type of delivery, conditions, temperature, blood versus crystalloid) and formulations to address detrimental consequences of specific ischemia-induced protein modification and function, (2) tailoring optimal cardioprotection strategies for individual populations (ie, age, sex, hypercholesterolemia, diabetes, hypertension),6,31,32 and (3) provide biomarkers and an understanding of disease mechanism for specific CAD patient population characteristics.…”