Proteomics reveals potential biomarkers of seed vigor in sugarbeet

Abstract

To unravel biomarkers of seed vigor, an important trait conditioning crop yield, a comparative proteomic study was conducted with sugarbeet seed samples of varying vigor as generated by an invigoration treatment called hydropriming and an aging treatment called controlled deterioration. Comparative proteomics revealed proteins exhibiting contrasting behavior between seed samples. Thus, 18 proteins were up-regulated during priming and down-regulated during aging and further displayed an up-regulation upon priming of the aged seeds, meaning that down-regulation of these spot volumes during aging was reversible upon subsequent priming. Also, 11 proteins exhibited the converse behavior characterized by a decrease and an increase of the spot volumes during priming and aging of the control seeds, respectively, and a decrease in the spot volumes upon priming of the aged seeds. The results underpinned the role in seed vigor of several metabolic pathways involved in lipid and starch mobilization, protein synthesis or the methyl cycle. They also corroborate previous studies suggesting that the glyoxylate enzyme isocitrate lyase, the capacity of protein synthesis and components of abscisic acid signaling pathways are likely contributors of seed vigor.

Dynamic Proteomics Emphasizes the Importance of Selective mRNA Translation and Protein Turnover during Arabidopsis Seed Germination

“…Notably, protein synthesis is absolutely required for Arabidopsis seed germination (20), and translational activity is strongly reduced in aged seeds that exhibit depressed seed vigor (26,119). Quantitatively, three distinct steps of mRNA translational activity are detected during seed germination (Table I).…”

“…Specifically, in Clusters 7 and 8, we found de novo synthesized proteins involved in energy production (TCA cycle, aconitase 3, malate dehydrogenase 1) or dedicated to the consumption of fatty acid (glyoxylate cycle, aconitase 3, isocitrate lyase, malate synthase). Most of these enzymes are important for seed vigor and seedling establishment (117)(118)(119). Cluster 8 contained proteins highly neosynthesized from 8 up to 48 HAI in a constant manner, with the exception of the very early steps of seed germination (supplemental Table S8).…”

Exogenous melatonin expediently modifies proteome of maize (Zea mays L.) embryo during seed germination

“…Bolded italics numbers above the bars indicate the total number of protein spots in the particular gels (drying after seed priming hydration/imbibition) (Gallardo et al 2001). Moreover, it was also reported to be a molecular seed vigour marker in Beta vulgaris seeds (Catusse et al 2011).…”

“…The seed variants: nt and H, were the reference for the detection of melatonin-associated proteins, since it is well known that already the conditioning with water provokes modifications in seed proteome (Catusse et al 2011;Wu et al 2011;Gallardo et al 2001).…”

“…Therefore, it is necessary to develop molecular markers improved vigour of primed seeds for commercial seed lots. Proteomics become widely applicable in seed research as a novel tool for protein characterisation and function analysis (Catusse et al 2011;Gallardo et al 2001;Wu et al 2011). Seeds develop differently in dicots and monocots, especially the changes induced by priming treatment.…”