Proteomics Profiling of Human Synovial Fluid Suggests Increased Protein Interplay in Early-Osteoarthritis (OA) That Is Lost in Late-Stage OA

Ali, Neserin; Turkiewicz, Aleksandra; Hughes, V.; Folkesson, Elin; Tjörnstand, J.; Neuman, Paul; Önnerfjord, Patrik; Englund, Martin

doi:10.1016/j.mcpro.2022.100200

Cited by 28 publications

(43 citation statements)

References 78 publications

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“…Ali et al showed in their study an overall higher protein expression in early-stage OA and a reduced response in late-stage OA [ 13 ].…”

Section: Synovial Fluid Physiopathologymentioning

confidence: 99%

“…Cartilage intermediate layer protein 1 (CILP) is a matrix protein that resides in the human articular cartilage and is involved in numerous diseases that affect cartilage. Its overexpression may lead to impaired chondrocyte growth and matrix repair [ 13 , 14 , 15 , 16 ]. Specifically, CILP has a relevant role in regulating the metabolism of the extracellular matrix (ECM).…”

Section: Synovial Proteomic Biomarkers In Oamentioning

confidence: 99%

“…Proteoglycan aggrecan (ACAN or PGCA) is a major component of the ECM of cartilaginous tissues [ 13 ]. The importance of this protein is demonstrated by the various skeletal malformations that are created if its gene (which appears to be widely expressed during the growth period) is under-expressed, with consequently reduced deposition of aggrecan into the cartilage context.…”

Section: Synovial Proteomic Biomarkers In Oamentioning

confidence: 99%

“…Decorin, belonging to the SLRP family of the ECM, is a proteoglycan with the important function of binding the collage fibres together, as well as being one of the biomarkers most involved in the pathogenesis of OA. Both the aggrecan and decorin proteoglycan levels were found reduced in both early and advanced OA synovial fluid; this is explained by the increased catabolic activity and by an important remodelling action of the synovial tissue [ 13 ]. It has been demonstrated that there is a positive correlation between the decorin levels and the WOMAC Osteoarthritis Index, whose respective altered values and results were found to be risk factors for OA.…”

Section: Synovial Proteomic Biomarkers In Oamentioning

confidence: 99%

“…Periostin, which also is associated with reparative processes involving cell adhesion and migration, was found to increase in both early- and late-stage OA in comparison to the controls, but there was a 3-fold difference in its levels when comparing early- and late-stage OA [ 13 ]. Recent evidence delineates an emerging role of periostin in OA since its expression after a knee injury is detrimental to the articular cartilage.…”

Section: Synovial Proteomic Biomarkers In Oamentioning

confidence: 99%

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Towards Precision Medicine for Osteoarthritis: Focus on the Synovial Fluid Proteome

Moretti

Bizzoca

Geronimo

et al. 2022

IJMS

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Osteoarthritis (OA) is a joint degenerative disease that most affects old age. The study of proteomics in synovial fluid (SF) has the task of providing additional elements to diagnose and predict the progress of OA. This review aims to identify the most significant biomarkers in the study of OA and to stimulate their routine use. Some of the major components of the ECM, such as proteoglycan aggrecan and decorin, were found considerably reduced in OA. Some biomarkers have proved useful for staging the temporality of OA: Periostin was found to be increased in early OA, while CRTA1 and MMPs were found to be increased in late OA. In its natural attempt at tissue regeneration, Collagen III was found to be increased in early OA while decreased in late OA. Some molecules studied in other areas, such as ZHX3 (oncological marker), LYVE1, and VEGF (lymph and angiogenesis markers), also have been found to be altered in OA. It also has been recorded that alteration of the hormonal pathway, using a dosage of PPAR-γ and RETN, can influence the evolution of OA. IL-1, one of the most investigated biomarkers in OA-SF, is not as reliable as a target of OA in recent studies. The study of biomarkers in SF appears to be, in combination with the clinical and radiological aspects, an additional weapon to address the diagnosis and staging of OA. Therefore, it can guide us more appropriately towards the indication of arthroplasty in patients with OA.

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“…Ali et al showed in their study an overall higher protein expression in early-stage OA and a reduced response in late-stage OA [ 13 ].…”

Section: Synovial Fluid Physiopathologymentioning

confidence: 99%

Section: Synovial Proteomic Biomarkers In Oamentioning

confidence: 99%

Section: Synovial Proteomic Biomarkers In Oamentioning

confidence: 99%

Section: Synovial Proteomic Biomarkers In Oamentioning

confidence: 99%

Section: Synovial Proteomic Biomarkers In Oamentioning

confidence: 99%

See 3 more Smart Citations

Towards Precision Medicine for Osteoarthritis: Focus on the Synovial Fluid Proteome

Moretti

Bizzoca

Geronimo

et al. 2022

IJMS

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Identification and quantification of degradome components in human synovial fluid reveals an increased proteolytic activity in knee osteoarthritis patients vs controls

et al. 2023

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Synovial fluid (SF) may contain cleavage products of proteolytic activities. Our aim was to characterize the degradome through analysis of proteolytic activity and differential abundance of these components in a peptidomic analysis of SF in knee osteoarthritis (OA) patients versus controls (n = 23). SF samples from end-stage knee osteoarthritis patients undergoing total knee replacement surgery and controls, that is, deceased donors without known knee disease were previously run using liquid chromatography mass spectrometry (LC-MS). This data was used to perform new database searches generating results for non-tryptic and semi-tryptic peptides for studies of degradomics in OA. We used linear mixed models to estimate differences in peptide-level expression between the two groups. Known proteolytic events (from the MEROPS peptidase database) were mapped to the dataset, allowing the identification of potential proteases and which substrates they cleave. We also developed a peptidecentric R tool, proteasy, which facilitates analyses that involve retrieval and mapping of proteolytic events. We identified 429 differentially abundant peptides. We found that the increased abundance of cleaved APOA1 peptides is likely a consequence of enzymatic degradation by metalloproteinases and chymase. We identified metalloproteinase, chymase, and cathepsins as the main proteolytic actors. The analysis indicated increased activity of these proteases irrespective of their abundance.

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Small Joint Organoids 3D Bioprinting: Construction Strategy and Application

Zhang,

Li,

Wang

et al. 2023

Small

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Osteoarthritis (OA) is a chronic disease that causes pain and disability in adults, affecting ≈300 million people worldwide. It is caused by damage to cartilage, including cellular inflammation and destruction of the extracellular matrix (ECM), leading to limited self‐repairing ability due to the lack of blood vessels and nerves in the cartilage tissue. Organoid technology has emerged as a promising approach for cartilage repair, but constructing joint organoids with their complex structures and special mechanisms is still challenging. To overcome these boundaries, 3D bioprinting technology allows for the precise design of physiologically relevant joint organoids, including shape, structure, mechanical properties, cellular arrangement, and biological cues to mimic natural joint tissue. In this review, the authors will introduce the biological structure of joint tissues, summarize key procedures in 3D bioprinting for cartilage repair, and propose strategies for constructing joint organoids using 3D bioprinting. The authors also discuss the challenges of using joint organoids’ approaches and perspectives on their future applications, opening opportunities to model joint tissues and response to joint disease treatment.

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Proteomics Profiling of Human Synovial Fluid Suggests Increased Protein Interplay in Early-Osteoarthritis (OA) That Is Lost in Late-Stage OA

Cited by 28 publications

References 78 publications

Towards Precision Medicine for Osteoarthritis: Focus on the Synovial Fluid Proteome

Towards Precision Medicine for Osteoarthritis: Focus on the Synovial Fluid Proteome

Identification and quantification of degradome components in human synovial fluid reveals an increased proteolytic activity in knee osteoarthritis patients vs controls

Small Joint Organoids 3D Bioprinting: Construction Strategy and Application

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