Proteomics, physiological, and biochemical analysis of cross tolerance mechanisms in response to heat and water stresses in soybean

Abstract

Water stress (WS) and heat stress (HS) have a negative effect on soybean plant growth and crop productivity. Changes in the physiological characteristics, proteome, and specific metabolites investigated on molecular and cellular functions were studied in two soybean cultivars exposed to different heat and water stress conditions independently and in combination. Leaf protein composition was studied using 2-DE and complemented with MALDI TOF mass spectrometry. While the two cultivars displayed genetic variation in response to water and heat stress, thirty-nine proteins were significantly altered in their relative abundance in response to WS, HS and combined WS+HS in both cultivars. A majority of these proteins were involved in metabolism, response to heat and photosynthesis showing significant cross-tolerance mechanisms. This study revealed that MED37C, a probable mediator of RNA polymerase transcription II protein, has potential interacting partners in Arabidopsis and signified the marked impact of this on the PI-471938 cultivar. Elevated activities in antioxidant enzymes indicate that the PI-471938 cultivar can restore the oxidation levels and sustain the plant during the stress. The discovery of this plant's development of cross-stress tolerance could be used as a guide to foster ongoing genetic modifications in stress tolerance.

Galactolipid and Phospholipid Profile and Proteome Alterations in Soybean Leaves at the Onset of Salt Stress

“…Furthermore, the integration of proteomics data with others from genomics, transcriptomics, or metabolomics provides wider scope in understanding biological processes ( Zhang and Kuster, 2019 ). In soybean, proteome studies on abiotic stress have been reported on flood, drought, heat, and salinity ( Das et al, 2016 ; Ji et al, 2016 ; Yin and Komatsu, 2017 ; Katam et al, 2020 ). A case in point is the integration of metabolome and proteome studies of the maize atg12 mutant which revealed a role for ATG12 in autophagic recycling by proteome remodeling and lipid turnover ( McLoughlin et al, 2018 ).…”

Soybean Plant Metabolism under Water Deficit and Xenobiotic and Antioxidant Agent Application

“…One situation of multiple stress factors that has been receiving attention in studies with is simultaneous water deficit and high temperature, which are almost dependent as it usually occurs at the same time in the field [ 13 ]. In these conditions, soybean was affected with greater intensity when water stress was imposed at higher temperatures [ 14 ].…”

Systems biology for crop improvement

“…Further, more abundance of proteins related to Calvin cycle, protein synthesis assembly and degradation, defense, and redox homeostasis contributed to better growth and yield in elevated CO 2 was reported (Maurya et al., 2020). In legumes, for example, soybean, proteomics together with physiological and biochemical analysis led to identification of cross‐tolerance mechanisms in response to heat and water stresses (Katam et al., 2020). The study reported elevated activities in antioxidant enzymes, such as increased ascorbate peroxidase enzyme, which restored oxidation levels and sustained soybean plants during stress.…”

“…The study reported elevated activities in antioxidant enzymes, such as increased ascorbate peroxidase enzyme, which restored oxidation levels and sustained soybean plants during stress. In addition, proteins such as MED37C, a probable mediator of RNA polymerase transcription II, were elevated in response to combined heat stress and water stress levels (Katam et al., 2020).…”