Proteomics of the Rodent Malaria Parasite Using Matrix-Assisted Laser Desorption/Ionization Quadrupole Ion Trap Time-of-Flight Tandem Mass Spectrometry

Abstract

Plasmodium berghei strain NK65 is a rodent malaria parasite species widely used as a model of the human-infectious malaria parasite. Because a rodent malaria parasite model allows issues to be addressed which would not be possible with human-infectious species, e.g., mode of action and in vivo screening, a convenient method to analyze the malaria parasite proteome is required. The proteins of P. berghei separated using two-dimensional polyacrylamide gel electrophoresis were analyzed using matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight tandem mass spectrometry.

Investigation of 5-(3-Trifluoromethylbenzylidene)thiazolidine-2,4-dione as a Matrix for Analyses of Biogenic Monoamine Transmitters Using MALDI-MS

“…[6][7][8][9][10] However, when measuring low molecular-weight compounds (LMWCs) using an α-cyano-4-hydroxycinnamic acid (CHCA) matrix, the presence of abundant matrix-derived ions in the low-mass range interferes with the detection of target ions due to suppression and overlapping. The discovery of new matrices suitable for the detection of LMWCs could allow for neurotransmitter analysis.…”