Proteomics of the <i>Roseobacter</i> clade, a window to the marine microbiology landscape

Christie-Oleza, Joseph Alexander; Armengaud, Jean

doi:10.1002/pmic.201500222

Cited by 13 publications

(7 citation statements)

References 136 publications

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“…The genus Dinoroseobacter is one of the most well-studied groups of marine bacteria [11,18]. Dinoroseobacter shibae , the type species of Dinoroseobacter , was isolated from the dinoflagellate Prorocentrum lima and lives in a symbiotic relationship with marine algae [19].…”

Section: Introductionmentioning

confidence: 99%

A Novel Roseosiphophage Isolated from the Oligotrophic South China Sea

Yang

Cai

et al. 2017

Viruses

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The Roseobacter clade is abundant and widespread in marine environments and plays an important role in oceanic biogeochemical cycling. In this present study, a lytic siphophage (labeled vB_DshS-R5C) infecting the strain type of Dinoroseobacter shibae named DFL12T, which is part of the Roseobacter clade, was isolated from the oligotrophic South China Sea. Phage R5C showed a narrow host range, short latent period and low burst size. The genome length of phage R5C was 77, 874 bp with a G+C content of 61.5%. Genomic comparisons detected no genome matches in the GenBank database and phylogenetic analysis based on DNA polymerase I revealed phylogenetic features that were distinct to other phages, suggesting the novelty of R5C. Several auxiliary metabolic genes (e.g., phoH gene, heat shock protein and queuosine biosynthesis genes) were identified in the R5C genome that may be beneficial to the host and/or offer a competitive advantage for the phage. Among siphophages infecting the Roseobacter clade (roseosiphophages), four gene transfer agent-like genes were commonly located with close proximity to structural genes, suggesting that their function may be related to the tail of siphoviruses. The isolation and characterization of R5C demonstrated the high genomic and physiological diversity of roseophages as well as improved our understanding of host–phage interactions and the ecology of the marine Roseobacter.

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Section: Introductionmentioning

confidence: 99%

A Novel Roseosiphophage Isolated from the Oligotrophic South China Sea

Yang

Cai

et al. 2017

Viruses

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“…Indeed, out of the top five most abundant proteins in the XP, OsmY and two ligand binding proteins were also predicted to be periplasmic (44,45) but were still found in the culture supernatant. Exoproteomics often captures a range of periplasmic proteins, especially ligand binding proteins (46)(47)(48), which suggests that the outer membrane is leaky. The mechanism of secretion for OsmY has not been studied, but it was used in biotechnology to deliver proteins into the medium via C-terminal fusion (49,50).…”

Section: Discussionmentioning

confidence: 99%

Mechanisms Involved in the Active Secretion of CTX-M-15 β-Lactamase by Pathogenic Escherichia coli ST131

Rangama

Lidbury

Holden

et al. 2021

Antimicrob Agents Chemother

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Infections caused by antimicrobial resistant bacterial pathogens are fast becoming an important global health issue. Strains of Escherichia coli are common causal agents of urinary tract infection and can carry multiple resistance genes. This includes the gene bla CTX-M-15 that encodes for an extended spectrum beta-lactamase (ESBL). While studying antimicrobial resistance (AMR) in the environment we isolated several strains of E. coli ST131 downstream of a waste water treatment plan (WWTP) in a local river. These isolates were surviving in the river sediment and characterisation proved that a multi-resistant phenotype was evident. Here, we show that E. coli strain 48 (river isolate ST131), provided a protective effect against a third-generation cephalosporin (cefotaxime) for a susceptible E. coli strain 33 (river isolate ST3576) through secretion of a functional ESBL into the growth medium. Furthermore, extracellular ESBL activity was stable for at least 24 h after secretion. Proteomic and molecular genetic analyses identified CTX-M-15 as the major secreted ESBL responsible for the observed protective effect. In contrast to previous studies, outer-membrane vesicles (OMVs) were not the route for CTX-M-15 secretion. Indeed, mutation of the Type I secretion system led to a significant reduction in the growth of the ESBL-producing strain as well as a significantly reduced ability to confer protective effect. We speculate that CTX-M-15 secretion, mediated through active secretion using molecular machinery provides a public goods service by facilitating the survival of otherwise susceptible bacteria in the presence of cefotaxime.

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“…Indeed, out of the top five most abundant proteins in the XP, OsmY and two ligand binding proteins were also predicted to be periplasmic (Yim and Villarejo, 1992;Oh et al, 1994), yet were still found in the culture supernatant. Exoproteomics often captures a range of periplasmic proteins, especially ligand binding proteins (Christie-Oleza and Armengaud, 2010;Christie-Oleza and Armengaud, 2015;Lidbury et al, 2016) which suggests that the outer membrane may be leaky. The mechanism of secretion for OsmY has not been studied but it was used in biotechnology to deliver proteins into the medium via C-terminal fusion (Qian et al, 2008;Bokinsky et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Mechanisms involved in the active secretion of CTX-M-15 β-lactamase by pathogenic E. coli ST131

Rangama

Lidbury

Holden

et al. 2021

Preprint

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Infections caused by antimicrobial resistant bacterial pathogens are fast becoming an important global health issue. Strains of Escherichia coli are common causal agents of urinary tract infection and can carry multiple resistance genes. This includes the gene blaCTX-M-15 that encodes for an extended spectrum β-lactamase (ESBL). While studying antimicrobial resistance (AMR) in the environment we isolated several strains of E. coli ST131 downstream of a WWTP in a local river. These isolates were surviving in the river sediment and characterisation proved that a multi-resistant phenotype was evident. Here, we show that E. coli strain 48 (river isolate ST131), provided a protective effect against a third-generation cephalosporin (cefotaxime) for a susceptible E. coli strain 33 (river isolate ST3576) through secretion of a functional ESBL into the growth medium. Furthermore, extracellular ESBL activity was stable for at least 24 h after secretion. Proteomic and molecular genetic analyses identified CTX-M-15 as the major secreted ESBL responsible for the observed protective effect. In contrast to previous studies, OMVs were not the sole route for CTX-M-15 secretion. Indeed, mutation of the Type I secretion system led to a significant reduction in the growth of the ESBL-producing strain as well as a significantly reduced ability to confer protective effect. We speculate that CTX-M-15 secretion, mediated through active secretion using molecular machinery provides a public goods service by facilitating the survival of otherwise susceptible bacteria in the presence of cefotaxime.

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Proteomics of the Roseobacter clade, a window to the marine microbiology landscape

Cited by 13 publications

References 136 publications

A Novel Roseosiphophage Isolated from the Oligotrophic South China Sea

A Novel Roseosiphophage Isolated from the Oligotrophic South China Sea

Mechanisms Involved in the Active Secretion of CTX-M-15 β-Lactamase by Pathogenic Escherichia coli ST131

Mechanisms involved in the active secretion of CTX-M-15 β-lactamase by pathogenic E. coli ST131

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