Proteomics of human embryonic stem cells

Hughes, Chris; Nuhn, A; Postovit, Lynne; Lajoie, Gilles

doi:10.1002/prca.201190052

Cited by 2 publications

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“…Biomaterials can fill several different roles in recapitulating the in vivo structural and/or chemical microenvironment. For example, hydrogels such as the widely used Matrigel [189] can act as ECM mimics to support tissue or organoid formation. [190][191][192] Similarly, a calcium phosphate-based biomaterial can mimic the inorganic part of the bone ECM.…”

Section: Biomaterials As Tools For On-chip Biologymentioning

confidence: 99%

Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research

Guttenplan

Birgani

Giselbrecht

et al. 2021

Adv Healthcare Materials

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In recent years, the use of microfabrication techniques has allowed biomaterials studies which were originally carried out at larger length scales to be miniaturized as so-called "on-chip" experiments. These miniaturized experiments have a range of advantages which have led to an increase in their popularity. A range of biomaterial shapes and compositions are synthesized or manufactured on chip. Moreover, chips are developed to investigate specific aspects of interactions between biomaterials and biological systems. Finally, biomaterials are used in microfabricated devices to replicate the physiological microenvironment in studies using so-called "organ-on-chip," "tissue-on-chip" or "disease-on-chip" models, which can reduce the use of animal models with their inherent high cost and ethical issues, and due to the possible use of human cells can increase the translation of research from lab to clinic. This review gives an overview of recent developments at the interface between microfabrication and biomaterials science, and indicates potential future directions that the field may take. In particular, a trend toward increased scale and automation is apparent, allowing both industrial production of micron-scale biomaterials and high-throughput screening of the interaction of diverse materials libraries with cells and bioengineered tissues and organs.

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Section: Biomaterials As Tools For On-chip Biologymentioning

confidence: 99%

Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research

Guttenplan

Birgani

Giselbrecht

et al. 2021

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…Nevertheless, it is crucial to isolate defined stem cell populations with known adequate differentiation potential, which is not a trivial task . An extensive review by Hughes et al highlights the importance of proteomics in human embryonic stem cell (hESC) research, providing a general overview of results obtained over the last few years from several proteomic analyses, namely in the growth of hESCs in vitro, posttranslation modification and membrane proteomics. This review reports fundamental information on the expressed proteins and their isoforms, giving the basics for investigation of stem cell interactions.…”

Section: Proteomics Of Stem Cell‐replacement Therapymentioning

confidence: 99%

Understanding regeneration through proteomics

et al. 2013

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Regeneration is a complex cellular process that, rather than simply forming a scar following injury, the animal forms a new functional tissue. Regeneration is a widespread process among metazoa, although not uniformly. Planaria, starfish, and some worms can regenerate most of their body, whereas many other species can only regenerate parts of specific tissues or fail to accomplish a functional regrowth, as is the case of mammals CNS. Research in regenerative medicine will possibly culminate in the regeneration of organs/tissues originally not prone to this process. Despite the complexity of the interactions and regulatory systems involved, the variety of tissues and organs these cells differentiate into has so far impaired the success of direct transplantation to restore damaged tissues. For this reason, a study, at the molecular level of the regeneration mechanisms developed by different animal models is likely to provide answers to why these processes are not readily activated in mammals. Proteomic-based approaches are being recognized as extremely useful to study of regeneration events, also because there is a relevant contribution of posttranscriptional processes that involve frequently the occurrence of a broad range of PTMs. The present review focuses on the significant knowledge brought up by proteomics in diverse aspects of regeneration research on different animal models, tissues, and organs.

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Proteomics of human embryonic stem cells

Abstract: This article was originally published in Proteomics 2011, 11, 675–690, DOI

Cited by 2 publications

References 0 publications

Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research

Chips for Biomaterials and Biomaterials for Chips: Recent Advances at the Interface between Microfabrication and Biomaterials Research

Understanding regeneration through proteomics

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