Proteomics of heavy metal toxicity in plants

Cvjetko, Petra; Zovko, Mira; Balen, Biljana

doi:10.2478/10004-1254-65-2014-2443

Cited by 39 publications

(20 citation statements)

References 137 publications

(178 reference statements)

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“…Heavy metals may affect cellular oxidation state, lipid peroxidation, breaking of DNA strands, protein expression and folding, degradation in proteasome, protein interactions, cell cycle and apoptosis; they are persistent and toxic, and they accumulate through the food chain (17,(23)(24)(25).…”

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confidence: 99%

Detection of heavy metals in common vegetables at Varaždin City Market, Croatia

Stančić

Vujević

Gomaz

et al. 2016

Archives of Industrial Hygiene and Toxicology

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The present study was aimed at the estimation of heavy metal content in vegetables sold at the city market of one of the densely populated Croatian cities, Varaždin, and to establish the relationship between their levels and possible sources of contamination. Twenty-eight samples of the most common diet vegetables (red and white potato, onion, carrot, common bean, lettuce, and cabbage) were randomly bought at the market in September and October 2013. Using the atomic absorption spectrometry method, concentrations of nine heavy metals (As, Cd, Cr, Cu, Hg, Mn, Ni, Pb, and Zn) were measured in the selected samples. The results showed that, in five out of 28 samples analysed, six concentrations exceeded the maximum levels provided for in the regulations: five for Pb and one for Cd. Maximum regulated levels for Pb were exceeded in two samples of red potato, two samples of common bean, and one sample of carrot (17.9 %), and for Cd in a sample of red potato (3.6 %). In conclusion, the cause of the overstepping of the maximum levels for Pb and Cd in the vegetables analysed was most likely the contaminated soil. The possible sources of soil contamination include traffic, nearby industry, floodwaters of rivers and streams, and the use of pesticides and fertilisers in agricultural production.

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mentioning

confidence: 99%

Detection of heavy metals in common vegetables at Varaždin City Market, Croatia

Stančić

Vujević

Gomaz

et al. 2016

Archives of Industrial Hygiene and Toxicology

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“…Many studies have shown positive effects of plant extracts (18), but it is also necessary to know what effects a particular component of the extract has. Furthermore, we must consider possible toxic effects from plant extracts (19). We therefore decided to use pure naringenin.…”

Section: Resultsmentioning

confidence: 99%

Naringenin ameliorates pathological changes in liver and kidney of diabetic mice: a preliminary study / Naringenin reducira histopatološke promjene u jetri i bubregu miševa s dijabetesom

Sirovina

Oršolić

Gregorović

et al. 2016

Archives of Industrial Hygiene and Toxicology

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The effect of naringenin, a flavonoid found in grapefruit, orange, and tomato, on lipid peroxidation and histopathological changes in the liver and kidneys of alloxan-induced diabetic mice were investigated. Two days after alloxan injection (75 mg kg -1 , i.v.), naringenin ethanolic solution (0.5 % v/v) was given to mice intraperitoneally (50 mg kg -1 per day) for seven days. Naringenin's impact on lipid peroxidation was measured by the 2-thiobarbituric acid test and histopathological changes were examined under a light microscope. Naringenin administration resulted in a significant decrease of lipid peroxidation level in liver and kidney tissue, as well as in a decreased number of vacuolated liver cells and degree of vacuolisation. Indications of tissue repair in kidney suggested that amelioration of diabetes-induced renal damage could be achieved over a longer period of time. Findings suggest that naringenin could be considered a dietary supplement in the prevention or treatment of diabetic complications and other diseases connected wih oxidative stress, and gives a hope that it could show similar effects in the treatment of diabetes in humans.

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“…Plant responses to HMs are present as a consequence of changes in protein expression. The qualitative and quantitative changes in proteins are a consequence of the expression of specific genes, which may not change the amount of total proteins content [19].…”

Section: Protein Assaysmentioning

confidence: 99%

Organic Soil Amendments in Assisted Phytoremediation of Heavy Metals Contaminated Soil - Basic Phytotoxicity Markers

Świątek¹,

Grobelak²

2018

Eng. Prot. Environ.

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Organiczne dodatki we wspomaganej fitoremediacji gleb zanieczyszczonych metalami ciężkimi -podstawowe markery fitotoksyczności Abiotic and biotic stressors induce a strong cellular response in plants, resulting in significant changes in plant cells metabolism. Knowledge in this area can help to develop very specific methods of stress decrease. For this purpose, it is necessary to find sensitive and accurate tools that will allow to estimate the level of stress in cells. The largest group of substances involved in stress response are proteins. The study of changes in the protein profile can yield the most answers concerning the different mechanisms of soil microflora tolerance and the adaptation of plants to unfavorable conditions. The knowledge of these mechanisms can significantly support the assisted phytoremediation of soils. The aim of the study was to investigate the effect of abiotic factors on plant stress response in Lupinus luteus grown on soil contaminated with heavy metals and amended with organic additives. The aim of the study was to determine the level of plant stress based on tests of plant stress proteins presence and a specific peroxidase activity in plants grown on soil contaminated with heavy metals (HMs). An acrylamide gel electrophoresis (SDS-PAGE) has been used in the study to separate specific proteins fractions (metallothioneins), accompanying stress factors. The results of peroxidase activity indicated that the organic soil amendments have an impact on reducing plant stress. The lowest dose of soil amendments reduced the amount of peroxidase by almost half in roots. This proves that adequate soil supplementation helps plants to tolerate stress. The SDS-PAGE analysis suggests that in the most stressful conditions the protein profile is significantly different from control and indicates additional small protein products in the range of 7÷20 kDa indicating, in accordance with the literature, presence of metallothioneins as response to plant stress. The applied methodology confirms that organic soil amendments reduced the level of HMs plants stress after organic amendments soil application.

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Proteomics of heavy metal toxicity in plants

Cited by 39 publications

References 137 publications

Detection of heavy metals in common vegetables at Varaždin City Market, Croatia

Detection of heavy metals in common vegetables at Varaždin City Market, Croatia

Naringenin ameliorates pathological changes in liver and kidney of diabetic mice: a preliminary study / Naringenin reducira histopatološke promjene u jetri i bubregu miševa s dijabetesom

Organic Soil Amendments in Assisted Phytoremediation of Heavy Metals Contaminated Soil - Basic Phytotoxicity Markers

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