Proteomics of edible mushrooms: A mini‐review

Abstract

Mushrooms are considered an important food for their traditionally famous nutritional and medicinal values, although much information about their potential at the molecular level is unfortunately unknown. Edible mushrooms include fungi that are either collected wild or cultivated. Many important species are difficult to cultivate but attempts have been made with varying degrees of success, with the results showing unsatisfactory economical cultivation methods. Recently, proteomic analysis has been developed as a powerful tool to study the protein content of fungi, particularly basidiomycetes. This mini-review article highlights the contribution of proteomics platforms to the study of edible mushrooms, focusing on the molecular mechanisms involved in developmental stages. This includes extracellular and cytoplasmic effector proteins that have potential or are involved in the synthesis of anticancer, antidiabetic, antioxidant, and antibiotic, in blood pressure control, in the supply of vitamins and minerals, and in other responses to environmental changes. The contribution of different proteomics techniques including classical and more advanced techniques is also highlighted.

Evaluation of functional and nutritional potential of a protein concentrate from Pleurotus ostreatus mushroom

“…Mushrooms produce a large number of proteins with biological and pharmacological activities (Al-Obaidi, 2016). The determination of the molecular weight of the peptides that comprise the protein concentrate may provide an overview regarding those properties.…”

Uncovering Prospective Role and Applications of Existing and New Nutraceuticals from Bacterial, Fungal, Algal and Cyanobacterial, and Plant Sources

“…In recent years, modern science has uncovered fungi as rich sources of nutraceuticals [53]. Mushrooms are rich in various types of bioactive compounds with functional properties as antioxidants [54,55], anticancer compounds [56], antibiotics [57], immunomodulators [58], hypocholesterolemics [59], and hypoglycemics [60].…”

Condiciones para el análisis de proteínas del micelio de Lentinula edodes obtenido por fermentación en estado líquido

“…En dichos estudios, se han realizado aproximaciones experimentales, las cuales incluyen el aislamiento de proteínas específicas y la posterior determinación de su actividad biológica [17], o aproximaciones holísticas, las cuales buscan dentro del proteoma completo la posible aplicación terapéutica [18]. Indistintamente del enfoque, es importante definir los protocolos que permitan una extracción de proteínas libres de contaminantes no proteicos en las etapas iniciales, los cuales puedan interferir en los métodos de análisis posteriores [19]. La importancia del protocolo de extracción en un experimento proteómico se puede resumir en la siguiente declaración: solo si se puede extraer y solubilizar una proteína, se tiene la posibilidad de detectarla e identificarla [20].…”